Molecular Biology Reports

, Volume 39, Issue 8, pp 8363–8371 | Cite as

Polymorphisms and expression of the chicken POU1F1 gene associated with carcass traits

  • Heng-Yong Xu
  • Yan Wang
  • Yi-Ping Liu
  • Ji-wen Wang
  • Qing Zhu


POU1F1 is an essential factor that regulates the development and reproduction of animal. The objective of the current research was to screen for polymorphism, expression of POU1F1 and their association with carcass quality traits. A total of 126 Erlang mountainous chickens from two strains (SD02 and SD03) were employed for testing. Seventeen single nucleotide polymorphisms (SNPs) were detected, but only two SNPs (g.96217999 T > C and g.96219442 C > T) were associated with carcass quality traits. In SD03 chicken, g.96217999 T > C genotypes were significantly associated with body weight (BW), carcass weight (CW), eviscerated weight (EW), and semi-eviscerated weight (SEW; P < 0.05), and was highly significantly associated with breast muscle weight (BMW) and abdominal fat weight (AW; P < 0.01). g.96219442 C > T was significantly associated with BW, EW, SEW (P < 0.05). However, these two SNPs were not significantly associated with any carcass traits in SD02 chicken. Diplotypes showed that in SD03 chicken, the haplotype [C: C] was the most favorable haplotype because it was associated with higher BW, CW, SEW, EW, BMW, and AW (P < 0.05). On the contrary, haplotype [T: T] was associated with lower carcass quality traits (P < 0.01). In addition, qRT-PCR revealed that at 13 weeks, the POU1F1 mRNA expression was significantly higher in breast muscle of cock compared to that of hens (P < 0.05), whereas there was no significant correlation between POU1F1 expression and carcass traits. These results suggested that POU1F1 could be a potential candidate gene for carcass traits in chicken.


POU1F1 gene Single nucleotide polymorphism mRNA expression Association Carcass trait 



The authors gratefully acknowledge Yao Zhang for help in managing the birds and collecting the data. This work was financially supported by the Chinese national key technologies R & D programme (Grant no: 2008BADB2B01), the Science Fund for Young Scholars in Sichuan Province (Grant no: ZQ 026-017), the Research Fund for Doctoral Program of Higher Education of China (Grant no: 20105103120006), the Scientific Research Fund of Sichuan Provincial Education Department (Grant no: 10ZB033).


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Heng-Yong Xu
    • 1
  • Yan Wang
    • 1
  • Yi-Ping Liu
    • 1
  • Ji-wen Wang
    • 1
  • Qing Zhu
    • 1
  1. 1.Laboratory of Animal Genetic and Breeding, Institute of Animal Genetics and BreedingSichuan Agriculture UniversityYa’anPeople’s Republic of China

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