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Polymorphisms and expression of the chicken POU1F1 gene associated with carcass traits

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Abstract

POU1F1 is an essential factor that regulates the development and reproduction of animal. The objective of the current research was to screen for polymorphism, expression of POU1F1 and their association with carcass quality traits. A total of 126 Erlang mountainous chickens from two strains (SD02 and SD03) were employed for testing. Seventeen single nucleotide polymorphisms (SNPs) were detected, but only two SNPs (g.96217999 T > C and g.96219442 C > T) were associated with carcass quality traits. In SD03 chicken, g.96217999 T > C genotypes were significantly associated with body weight (BW), carcass weight (CW), eviscerated weight (EW), and semi-eviscerated weight (SEW; P < 0.05), and was highly significantly associated with breast muscle weight (BMW) and abdominal fat weight (AW; P < 0.01). g.96219442 C > T was significantly associated with BW, EW, SEW (P < 0.05). However, these two SNPs were not significantly associated with any carcass traits in SD02 chicken. Diplotypes showed that in SD03 chicken, the haplotype [C: C] was the most favorable haplotype because it was associated with higher BW, CW, SEW, EW, BMW, and AW (P < 0.05). On the contrary, haplotype [T: T] was associated with lower carcass quality traits (P < 0.01). In addition, qRT-PCR revealed that at 13 weeks, the POU1F1 mRNA expression was significantly higher in breast muscle of cock compared to that of hens (P < 0.05), whereas there was no significant correlation between POU1F1 expression and carcass traits. These results suggested that POU1F1 could be a potential candidate gene for carcass traits in chicken.

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Acknowledgments

The authors gratefully acknowledge Yao Zhang for help in managing the birds and collecting the data. This work was financially supported by the Chinese national key technologies R & D programme (Grant no: 2008BADB2B01), the Science Fund for Young Scholars in Sichuan Province (Grant no: ZQ 026-017), the Research Fund for Doctoral Program of Higher Education of China (Grant no: 20105103120006), the Scientific Research Fund of Sichuan Provincial Education Department (Grant no: 10ZB033).

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Authors and Affiliations

  1. Laboratory of Animal Genetic and Breeding, Institute of Animal Genetics and Breeding, Sichuan Agriculture University, Xingkang Road 46#, Ya’an, Sichuan, 625014, People’s Republic of China

    Heng-Yong Xu, Yan Wang, Yi-Ping Liu, Ji-wen Wang & Qing Zhu

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  1. Heng-Yong Xu
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  2. Yan Wang
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  3. Yi-Ping Liu
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Correspondence to Qing Zhu.

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Heng-Yong Xu and Yan Wang contributed equally to this work.

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Xu, HY., Wang, Y., Liu, YP. et al. Polymorphisms and expression of the chicken POU1F1 gene associated with carcass traits. Mol Biol Rep 39, 8363–8371 (2012). https://doi.org/10.1007/s11033-012-1686-9

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  • DOI: https://doi.org/10.1007/s11033-012-1686-9

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