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Cloning and function analysis of a drought-inducible gene associated with resistance to Curvularia leaf spot in maize

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Abstract

ZmDIP was cloned and its function against Curvularia lunata was analyzed, according to a previous finding on a drought-inducible protein in resistant maize identified through MALDI-TOF-MS/MS. The ZmDIP expression varied in roots, leaf sheaths, and young, as well as old, leaves of different maize inbred lines. The ZmDIP transcript level changed in leaves over the course of time after inoculation with C. lunata. A prokaryotic expression analysis demonstrated that the gene can regulate the salt stress tolerance of Escherichia coli. The ZmDIP transient expression in the maize leaf showed that the gene was also linked to leaf resistance against the C. lunata infection. ZmDIP-mediated ROS and ABA signaling pathways were inferred to be closely associated with maize leaf resistance to the pathogen infection.

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Acknowledgement

The current work was supported by the China Agriculture Research System (CARS-02) and the National Natural Science Foundation of China (30871610, 30971949, and 31171798).

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Authors and Affiliations

  1. Department of Plant Science, School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai, 200240, China

    Jiewei Zhu, Xiuli Huang, Shigang Gao & Jie Chen

  2. School of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, China

    Tong Liu

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  1. Jiewei Zhu
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  2. Xiuli Huang
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  3. Tong Liu
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  4. Shigang Gao
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  5. Jie Chen
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Correspondence to Jie Chen.

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Jiewei Zhu, Xiuli Huang—Joint first author.

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Zhu, J., Huang, X., Liu, T. et al. Cloning and function analysis of a drought-inducible gene associated with resistance to Curvularia leaf spot in maize. Mol Biol Rep 39, 7919–7926 (2012). https://doi.org/10.1007/s11033-012-1636-6

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  • DOI: https://doi.org/10.1007/s11033-012-1636-6

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