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Molecular Biology Reports

, Volume 39, Issue 6, pp 6737–6743 | Cite as

Hydroquinone-induced apoptosis of human lymphocytes through caspase 9/3 pathway

  • Ji-Sook Lee
  • Eun Ju Yang
  • In Sik Kim
Article

Abstract

Hydroquinone (HQ) is a major benzene metabolite, which is produced after benzene biotransformation. In this study, we investigated the toxic effect of HQ on lymphocytes. HQ significantly induced the apoptosis of lymphocytes isolated from normal peripheral blood in both dose and time dependent courses. Volatile organic compounds such as benzene, phenol, formaldehyde, o- and p-xylene, and toluene have no effect on lymphocyte apoptosis. HQ induced the cleavage of procaspase 3 and procaspase 9, indicating activation of the pro-apoptotic enzymes. Supernatant was collected from normal lymphocytes after HQ treatment and it significantly induced the apoptosis of normal lymphocytes as compared to supernatant collected from normal lymphocytes without HQ treatment. HQ reduced the secretion of MCP-1, IL-6 and IL-8 increased by in vitro incubation, although benzene and phenol are not effective in cytokine production. HQ increased the intracellular ROS production of lymphocytes. Benzene and phenol also increased the ROS production. In summary, HQ has a cytotoxic effect on lymphocytes by apoptosis induction and the pro-apoptotic signaling is involved in caspase 9/3 pathway. Our results demonstrated that HQ induces apoptosis by activating caspases 9/3 pathway and that the toxic effect seems to be dependent on lymphocyte metabolism.

Keywords

Hydroquinone Apoptosis Lymphocyte Cytotoxicity Cytokine 

Abbreviations

HQ

Hydroquinone

MCP-1

Monocyte chemoattractant protein-1

VEGF

Vascular endothelial growth factor

FBS

Fetal bovine serum

PI

Propidium iodide

LPS

Lipopolysaccharide

PKC δ

Protein kinase C δ

ROS

Reactive oxygen species

Notes

Acknowledgements

This paper was supported by Wonkwang Health Science University in 2011.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of Clinical Laboratory ScienceWonkwang Health Science UniversityIksanRepublic of Korea
  2. 2.Department of Clinical Laboratory Science, College of Health and TherapyDaegu Hanny UniversityGyeongsanRepublic of Korea
  3. 3.Department of Biomedical Laboratory Science, School of MedicineEulji UniversityDaejeonRepublic of Korea

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