Molecular Biology Reports

, Volume 39, Issue 6, pp 6723–6730 | Cite as

Evaluation of ERα and VDR gene polymorphisms in relation to bone mineral density in Turkish postmenopausal women

  • Ozlem Kurt
  • Hulya Yilmaz-Aydogan
  • Mehmet Uyar
  • Turgay Isbir
  • Mehmet Fatih Seyhan
  • Ayse Can


It has been suggested that the estrogen receptor alpha (ERα) and vitamin D receptor (VDR) genes as possibly implicated in reduced bone mineral density (BMD) in osteoporosis. The present study investigated the relation of ERα PvuII/XbaI polymorphisms and VDR FokI/TaqI polymorphisms with BMD in Turkish postmenopausal women. Eighty-one osteoporotic and 122 osteopenic postmenopausal women were recruited. For detection of the polymorphisms, polymerase chain reaction-restriction fragment lenght polymorphism techniques have been used. BMD was measured at the lumbar spine and hip by dual-energy X-ray absorptiometry. Distributions of ERα (PvuII dbSNP: rs2234693, XbaI dbSNP: rs9340799) and VDR genotypes (FokI dbSNP rs10735810, TaqI dbSNP: rs731236) were similar in study population. Although overall prevalence of osteoporosis had no association with these genotypes, the prevalence of decreased femoral neck BMD values were higher in the subjects with ERα PvuII “PP” and ERα XbaI “XX” genotypes than in those with “Pp/pp” genotypes and “xx” genotype, respectively (P < 0.05). Furthermore, subjects with VDR FokI “FF” genotype had lower BMD values of femoral neck and total hip compared to those with “Ff” genotype (P < 0.05). In the logistic regression analysis, we confirmed the presence of relationships between the VDR FokI “FF” genotypes, BMI ≤ 27.5, age ≥ 55 and the increased risk of femoral neck BMD below 0.8 value in postmenopausal women. The present data suggests that the ERα PvuII/XbaI and VDR FokI polymorphisms may contribute to the determination of bone mineral density in Turkish postmenopausal women.


Osteoporosis Estrogen receptor Vitamin D receptor Polymorphism Bone mineral density 



Estrogen receptor alpha


Vitamin D receptor


Bone mineral density


Polymerase chain reaction-restriction fragment lenght polymorphism


Single nucleotid polymorphism


1α,25-dihydroxyvitamin D3


Body mass index


Hardy–Weinberg equilibrium


Dual energy X-ray absorptiometry



The present work was supported by the Scientific Research Projects Coordination Unit of Istanbul University (Project No: T-68/15122006).


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ozlem Kurt
    • 1
  • Hulya Yilmaz-Aydogan
    • 2
  • Mehmet Uyar
    • 3
  • Turgay Isbir
    • 4
  • Mehmet Fatih Seyhan
    • 2
  • Ayse Can
    • 1
  1. 1.Department of Biochemistry, Faculty of PharmacyIstanbul UniversityIstanbulTurkey
  2. 2.Department of Molecular Medicine, The Institute of Experimental MedicineIstanbul UniversityIstanbulTurkey
  3. 3.Department of Phsycial Medicine and RehabilitationUskudar State HospitalIstanbulTurkey
  4. 4.Department of Medical Biology, Medical FacultyYeditepe UniversityIstanbulTurkey

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