Molecular Biology Reports

, Volume 39, Issue 5, pp 5977–5984 | Cite as

Variant allele of CHEK2 is associated with a decreased risk of esophageal cancer lymph node metastasis in a Chinese population

  • Haiyong Gu
  • Wanshan Qiu
  • Ying Wan
  • Guowen Ding
  • Weifeng Tang
  • Chao Liu
  • Yijun Shi
  • Yijang Chen
  • Suocheng Chen


Growing evidence suggests that the checkpoint kinase 2 (CHEK2) signaling pathway occupies a central position in the signaling networks of DNA-damage signaling. Many functional and molecular epidemiological studies have evaluated the association between genetic variants of CHEK2 and various cancers. To evaluate the relationship between CHEK2 functional genetic variants and esophageal cancer risk and the risk of lymph node metastasis among a Chinese population. We genotyped CHEK2 rs738722, rs2236141 and rs2236142 single nucleotide polymorphisms (SNPs) using the matrix assisted laser desorption/ionization time-of-flight mass spectrometry assay in a case–controlled study, including 380 esophageal cancer cases and 380 healthy controls in a Chinese population. We found that none of the three polymorphisms achieved significant difference in their distributions between esophageal cancer cases and controls. Multiple logistic regression analyses revealed that esophageal cancer risk was not associated significantly with the variant genotypes of the three CHEK2 polymorphisms as compared with their wild-type genotypes. However, we found that functional variant rs738722 and rs2236142 in CHEK2 might contribute to susceptibility to lymph node metastasis. Our data did not support a significant association between CHEK2 SNPs and the risk of esophageal cancer. Functional variant CHEK2 rs738722 and rs2236142 might contribute to lymph node metastasis susceptibility. The CT allele of SNP rs738722 and the GC allele of SNP rs2236142 might be a protective factor of the risk for lymph node metastasis of esophageal cancer.


CHEK2 Polymorphisms Esophageal cancer Lymph node metastasis Molecular epidemiology 



Confidence interval


Checkpoint kinase 2


Linkage disequilibrium


Odds ratio


Single nucleotide polymorphisms



This work was supported in part by National Natural Science Foundation of China (81101889), the Jiangsu Province Natural Science Foundation (BK2009207) and Social Development Foundation of Zhenjiang (SH2010017).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Haiyong Gu
    • 1
  • Wanshan Qiu
    • 2
  • Ying Wan
    • 3
  • Guowen Ding
    • 1
  • Weifeng Tang
    • 1
  • Chao Liu
    • 1
  • Yijun Shi
    • 1
  • Yijang Chen
    • 4
  • Suocheng Chen
    • 1
  1. 1.Department of Cardiothoracic SurgeryAffiliated People’s Hospital of Jiangsu UniversityZhenjiangChina
  2. 2.Children’s Hospital of Fudan UniversityShanghaiChina
  3. 3.Department of Clinical LaboratoryThe Affiliated Hospital of Jiangsu UniversityZhenjiangChina
  4. 4.Department of Thoracic & Cardiac SurgeryThe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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