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Comparative proteomics analysis of serum proteins in ulcerative colitis patients

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Abstract

In the present study, we investigated the differentially expressed proteins associated with ulcerative colitis (UC) using proteomic methods. Two-dimensional electrophoresis (2-DE) technology was performed to separate the total proteins of ulcerative tissues from those of the normal tissues of UC patients. PDQuest software was applied to analyze the obtained 2-DE images. Candidate protein spots between the two groups were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics analysis. The well resolution and reproducible 2-DE patterns of UC and normal tissues were established. Of the 12 differentially expressed proteins, 9 were successfully identified, of which 6 proteins were up-regulated including apolipoprotein C-III, haptoglobin, receptor tyrosine kinase, aldehyde reductase, pericentriolar material 1, and heat shock factor protein 2, and 3 were down-regulated including keratin, filamin A-interacting protein 1, and tropomyosin 3. These identified proteins were related to hormonal modulation, immune response, oxidative stress, and signal conduction. The 2-DE protein expression profile of the UC tissues displays an obvious difference from that of the normal controls. Various proteins may be involved in the occurrence of UC.

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Acknowledgment

This work was supported by the grants from the National Key Lab Proteomics, China (No. 2009-J-0801).

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Authors and Affiliations

  1. Department of Gastenerology, 309 Hospital, PLA, Beijing, 100091, China

    Nan Li, Xueming Wang, Yuefan Zhang, Junshan Zhai, Tuo Zhang & Kaihua Wei

  2. Department of Pharmacy, 309 Hospital, PLA, Beijing, 100091, China

    Xueming Wang

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  1. Nan Li
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  2. Xueming Wang
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Correspondence to Nan Li.

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Li, N., Wang, X., Zhang, Y. et al. Comparative proteomics analysis of serum proteins in ulcerative colitis patients. Mol Biol Rep 39, 5659–5667 (2012). https://doi.org/10.1007/s11033-011-1373-2

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  • DOI: https://doi.org/10.1007/s11033-011-1373-2

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