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Molecular Biology Reports

, Volume 39, Issue 5, pp 5659–5667 | Cite as

Comparative proteomics analysis of serum proteins in ulcerative colitis patients

  • Nan Li
  • Xueming Wang
  • Yuefan Zhang
  • Junshan Zhai
  • Tuo Zhang
  • Kaihua Wei
Article

Abstract

In the present study, we investigated the differentially expressed proteins associated with ulcerative colitis (UC) using proteomic methods. Two-dimensional electrophoresis (2-DE) technology was performed to separate the total proteins of ulcerative tissues from those of the normal tissues of UC patients. PDQuest software was applied to analyze the obtained 2-DE images. Candidate protein spots between the two groups were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics analysis. The well resolution and reproducible 2-DE patterns of UC and normal tissues were established. Of the 12 differentially expressed proteins, 9 were successfully identified, of which 6 proteins were up-regulated including apolipoprotein C-III, haptoglobin, receptor tyrosine kinase, aldehyde reductase, pericentriolar material 1, and heat shock factor protein 2, and 3 were down-regulated including keratin, filamin A-interacting protein 1, and tropomyosin 3. These identified proteins were related to hormonal modulation, immune response, oxidative stress, and signal conduction. The 2-DE protein expression profile of the UC tissues displays an obvious difference from that of the normal controls. Various proteins may be involved in the occurrence of UC.

Keywords

Ulcerative colitis Two dimensional electrophoresis Mass spectrogram Serum 

Notes

Acknowledgment

This work was supported by the grants from the National Key Lab Proteomics, China (No. 2009-J-0801).

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Nan Li
    • 1
  • Xueming Wang
    • 1
    • 2
  • Yuefan Zhang
    • 1
  • Junshan Zhai
    • 1
  • Tuo Zhang
    • 1
  • Kaihua Wei
    • 1
  1. 1.Department of Gastenerology309 Hospital, PLABeijingChina
  2. 2.Department of Pharmacy309 Hospital, PLABeijingChina

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