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Analysis of gene expression profile of Arabidopsis genes under trichloroethylene stresses with the use of a full-length cDNA microarray

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Abstract

Trichloroethylene (TCE) is a widespread and persistent environmental contaminant. Plants are able to take up a range of harmful organic compounds, including some of the most abundant environmental pollutants like TCE. In this study, complementary DNA microarrays were constructed to have a better view of transcript expression in Arabidopsis thaliana during TCE-induced stress. The microarray analysis demonstrated the complexity of gene expression patterns resulting from TCE. A total of 1,020 transcripts were differentially up-regulated by TCE. Those genes might specifically contribute to the TCE transformation, conjugation, and compartmentation in plant. This study provides informative preliminary data for more in-depth analyses of TCE tolerance in Arabidopsis thaliana.

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Acknowledgments

The research was supported by Hi-tech Research and Development Program (863) of China (2008AA10Z401) and Shanghai Natural Science Foundation (11ZR1432600).

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Authors and Affiliations

  1. Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People’s Republic of China

    Bo Zhu, Ri-He Peng, Ai-Sheng Xiong, Xiao-Yan Fu, Wei Zhao, Yong-Sheng Tian, Xiao-Fen Jin, Yong Xue, Jing Xu, Hong-Juan Han, Chen Chen, Jian-Jie Gao & Quan-Hong Yao

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  1. Bo Zhu
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Correspondence to Quan-Hong Yao.

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Zhu, B., Peng, RH., Xiong, AS. et al. Analysis of gene expression profile of Arabidopsis genes under trichloroethylene stresses with the use of a full-length cDNA microarray. Mol Biol Rep 39, 3799–3806 (2012). https://doi.org/10.1007/s11033-011-1157-8

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  • DOI: https://doi.org/10.1007/s11033-011-1157-8

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