Analysis of gene expression profile of Arabidopsis genes under trichloroethylene stresses with the use of a full-length cDNA microarray
- 245 Downloads
Trichloroethylene (TCE) is a widespread and persistent environmental contaminant. Plants are able to take up a range of harmful organic compounds, including some of the most abundant environmental pollutants like TCE. In this study, complementary DNA microarrays were constructed to have a better view of transcript expression in Arabidopsis thaliana during TCE-induced stress. The microarray analysis demonstrated the complexity of gene expression patterns resulting from TCE. A total of 1,020 transcripts were differentially up-regulated by TCE. Those genes might specifically contribute to the TCE transformation, conjugation, and compartmentation in plant. This study provides informative preliminary data for more in-depth analyses of TCE tolerance in Arabidopsis thaliana.
KeywordscDNA Microarray Trichloroethylene Arabidopsis thaliana Phytoremediation Gene expression
The research was supported by Hi-tech Research and Development Program (863) of China (2008AA10Z401) and Shanghai Natural Science Foundation (11ZR1432600).
- 4.NTP (National Toxicology Program) (1990) Carcinogenesis studies of trichloroethylene in F344/N rats and B6C3F1 mice. NTP Technical ReportGoogle Scholar
- 5.ATSDR (1997) US Department of Health and Human Services, Agency for Toxic Substances and Disease Registry. Toxicologic profile for trichloroethyleneGoogle Scholar
- 7.Anderson TA, Walton BT (1995) Comparative fate of 14C TCE in the root zone of plants from a former solvent disposal site. Environ Toxicol Chem 14:2041–2047Google Scholar
- 25.Komives T, Gullner G (2005) Phase I xenobiotic metabolic systems in plants Z. Naturforsch 60:179–185Google Scholar