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Generation and analysis of expressed sequence tags from the bone marrow of Chinese Sika deer

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Abstract

Sika deer is one of the best-known and highly valued animals of China. Despite its economic, cultural, and biological importance, there has not been a large-scale sequencing project for Sika deer to date. With the ultimate goal of sequencing the complete genome of this organism, we first established a bone marrow cDNA library for Sika deer and generated a total of 2,025 reads. After processing the sequences, 2,017 high-quality expressed sequence tags (ESTs) were obtained. These ESTs were assembled into 1,157 unigenes, including 238 contigs and 919 singletons. Comparative analyses indicated that 888 (76.75%) of the unigenes had significant matches to sequences in the non-redundant protein database, In addition to highly expressed genes, such as stearoyl-CoA desaturase, cytochrome c oxidase, adipocyte-type fatty acid-binding protein, adiponectin and thymosin beta-4, we also obtained vascular endothelial growth factor-A and heparin-binding growth-associated molecule, both of which are of great importance for angiogenesis research. There were 244 (21.09%) unigenes with no significant match to any sequence in current protein or nucleotide databases, and these sequences may represent genes with unknown function in Sika deer. Open reading frame analysis of the sequences was performed using the getorf program. In addition, the sequences were functionally classified using the gene ontology hierarchy, clusters of orthologous groups of proteins and Kyoto encyclopedia of genes and genomes databases. Analysis of ESTs described in this paper provides an important resource for the transcriptome exploration of Sika deer, and will also facilitate further studies on functional genomics, gene discovery and genome annotation of Sika deer.

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Abbreviations

ESTs:

Expressed sequence tags

ORF:

Open reading frame

GO:

Gene ontology

COGs:

Clusters of orthologous groups of proteins

KEGG:

Kyoto encyclopedia of genes and genomes

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Acknowledgment

This work was supported by the National Key Technology R&D Program of China (No. 2007BAI38B06).

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Authors and Affiliations

  1. School of Public Health, Jilin University, Changchun, 130021, China

    Baojin Yao & Juan Li

  2. Center for New Medicine Research, Changchun University of Chinese Medicine, Changchun, 130117, China

    Yu Zhao & Mei Zhang

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  1. Baojin Yao
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  2. Yu Zhao
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  3. Mei Zhang
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Correspondence to Yu Zhao or Juan Li.

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Yao, B., Zhao, Y., Zhang, M. et al. Generation and analysis of expressed sequence tags from the bone marrow of Chinese Sika deer. Mol Biol Rep 39, 2981–2990 (2012). https://doi.org/10.1007/s11033-011-1060-3

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