Molecular Biology Reports

, Volume 39, Issue 2, pp 1957–1962 | Cite as

Molecular cloning and expression analysis of a RGA-like gene responsive to plant hormones in Brassica napus

  • Yong Gao
  • Jianmin Chen
  • Yun Zhao
  • Tingting Li
  • Maolin Wang


DELLA proteins are negative regulators of GA-induced growth. DELLA protein family is characterized by a DELLA domain essential for GA-dependent proteasomal degradation of DELLA repressors. A full-length cDNA encoding a putative DELLA protein with high sequence homology to Arabidopsis thaliana RGA (AtRGA), designated as BnRGA, was isolated from Brassica napus. The full-length cDNA of BnRGA contained a 1,740 bp open reading frame (ORF) encoding a precursor protein of 579 amino acid residues. Comparative and bioinformatics analyses revealed that BnRGA showed a high degree of homology with DELLA proteins and contained the DELLA domain, TVHYNP domain, VHIID domain and RVER domain. Using real-time PCR, the expression patterns of BnRGA and two our previously isolated genes, BnGID1a and BnSLY1 in B. napus, were analyzed by adding exogenous gibberellins acid-3 (GA3), GA biosynthetic inhibitor paclobutrazol (PAC) and abscisic acid (ABA). The results showed that the expression of BnGID1a and BnSLY1 was down-regulated after treated by GA3 and induced by PAC and ABA. These results suggest that the expression of BnGID1a and BnSLY1 may be negatively regulated by the level of endogenous GA in B. napus. Moreover, BnRGA was not significantly regulated by GA3, PAC and ABA in the low concentrations. These suggest that GA-GID1-SCF-DELLA complex may have a mechanism of self-regulation, thereby preserving the stability of the expression level of BnRGA in B. napus.


DELLA protein Gene cloning Gibberellins Abscisic acid Expression Brassica napus 





Abscisic acid




Gibberellin insensitive dwarf 1



This work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions, the National ‘863’ Program (SN: 2001AA241104) and the 10th ‘five-year’ key task project in crop breeding of Sichuan Province (SN: 200107001).

Supplementary material

11033_2011_943_MOESM1_ESM.jpg (1.8 mb)
Fig. S1 Comparison of the putative amino acid sequences of BnRGA with some representative DELLA proteins. The identical amino acids were showed in white with black background and the conserved amino acids were showed in white with gray background. The specific sites for DELLA domain, TVHYNP domain, VHIID domain and RVER donain were presented. The aligned DELLA proteins sequences were from A. thaliana (RGA1, GenBank accession no. NP_178266; GAI, GenBank accession no. NP_172945; RGL1, GenBank accession no. NP_176809; RGL2, GenBank accession no. NP_186995; RGL3, GenBank accession no. NP_197251). Supplementary material 1 (JPEG 1849 kb)
11033_2011_943_MOESM2_ESM.jpg (198 kb)
Fig. S2 Phylogenetic tree analysis of BnRGA from plants by MEGA version 3.1. The neighbor-joining method was used to construct the tree. Numbers on nodes indicated the bootstrap values after 1,000 replicates. The genes used in phylogenetic tree analysis were from plants including A. thaliana RGA1 GenBank accession no. NM_126218; GAI GenBank accession no. NM_101361; RGL1 GenBank accession no. NM_105306; RGL2 GenBank accession no. NM_111216; RGL3 GenBank accession no. NM_121755. (JPEG 197 kb)


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yong Gao
    • 1
  • Jianmin Chen
    • 1
  • Yun Zhao
    • 2
  • Tingting Li
    • 2
  • Maolin Wang
    • 2
  1. 1.College of Bioscience and Biotechnology, Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhouPeople’s Republic of China
  2. 2.Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life SciencesSichuan UniversityChengduPeople’s Republic of China

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