Polymorphisms of diacylglycerol acyltransferase 2 gene and their relationship with growth traits in goats

Abstract

Diacylglycerol acyltransferase (DGAT) plays a critical role in the synthesis of triacylglycerol. In this study, PCR-SSCP and DNA sequencing methods were employed to screen the genetic variations of DGAT-2 gene in 299 goats from three breeds (Boer goat, Chinese Xuhuai white goat and Chinese Haimen goat). Three fragments of DGAT-2 gene were investigated, only exon 3 of DGAT-2 gene showed polymorphism. The alignment between nucleotide sequences of NM_205793.2 in GenBank and the sequencing results of three PCR products with different patterns revealed that there was one mutation (A → G) in exon 3 of DGAT-2 gene, which resulted in amino acid change (Lys → Arg) and constructed two genotypes (AA, AB). The frequencies of allele A and genotype AA were dominant in all three breeds. And there was no significant difference for genotypic and allelic frequencies among the three breeds. The genotype distributions were in good agreement with Hardy–Weinberg equilibrium (P > 0.05) in each breed. Significant statistical differences were only found in withers heights (P < 0.05) in Xuhuai goat between genotypes. The results indicated that individuals with genotype AA were significantly higher than those of individuals with genotype AB in withers height (P < 0.05). No polymorphism was detected in the intron 3, exon 8 and 3′ flanking region. So we suggested that DGAT-2 gene had the close relationship with growth traits in goats. And this mutation could be used as a perfect molecular marker for marker-assisted selection (MAS) in animal genetics and breeding.

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References

  1. 1.

    Bell RM, Coleman RA (1980) Enzymes of glycerolipid synthesis in eukaryotes. Annu Rev Biochem 49:459–487

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    Weiss SB, Kennedy EP, Kiyasu JY (1960) The enzymatic synthesis of triglycerides. J Biol Chem 235:40–44

    PubMed  CAS  Google Scholar 

  3. 3.

    Suzuki R, Tobe K, Aoyama M, Sakamoto K, Ohsugi M, Kamei N, Nemoto S, Inoue A, Ito Y, Uchida S, Hara K, Yamauchi T, Kubota N, Terauchi Y, Kadowaki T (2005) Expression of DGAT2 in white adipose tissue is regulated by central leptin action. J Biol Chem 280:3331–3337

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Farese RV Jr, Cases S, Smith SJ (2000) Triglyceride synthesis: insights from the cloning of diacylglycerol acyltransferase. Curr Opin Lipidol 11:229–234

    PubMed  Article  CAS  Google Scholar 

  5. 5.

    Lardizabal KD, Mai JT, Wagner NW, Wyrick A, Voelker T, Hawkins DJ (2001) DGAT2 is a new diacylglycerol acyltransferase gene family. J Biol Chem 276(42):38862–38869

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Cases S, Stone SJ, Zhou P, Tow B, Lardizabal KD, Voelker T, Farese RV Jr (2001) Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase and related family members. J Biol Chem 276(42):38870–38876

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    Winter A, van Eckeveld M, Bininda-Emonds OR, Habermann FA, Fries R (2003) Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals. Cytogenet Genome Res 102(124):42–47

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Stone SJ, Myers HM, Watkins SM, Brown BE, Feingold KR, Elias PM, Farese RV Jr (2004) Lipopenia and skin barrier abnormalities in DGAT2-deficient mice. J Biol Chem 279:11767–11776

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Meegalla RL, Billheimer JT, Cheng D (2002) Concerted elevation of acylcoenzyme A: diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin. Biochem Biophys Res Commun 298(3):317–323

    PubMed  Article  CAS  Google Scholar 

  10. 10.

    Vaziri ND, Kim CH, Dang B, Zhan CD, Liang K (2004) Downregulation of hepatic-acy-CoA: diglycerol acyltransferase in chronicrenal failure. Am J Physio Renal Physiol 287(1):90–94

    Article  Google Scholar 

  11. 11.

    Mao HX, Chen H, Chen FY, Zhang CL, Wang XZ, Wang JQ (2008) Association of polymorphisms of DGAT2 gene with growth traits in Jiaxian red cattle. Hereditas (Beijing) 30(3):329–332

    Article  CAS  Google Scholar 

  12. 12.

    Xu XR, Gao X, Xu SZ, Zhang YH, Ren HY, Li JY (2005) Polymorphisms at intron 6 of cattle DGAT-2 gene and its associations with economic traits in cattle. Chinese J Anim Vet Sci 36(10):981–986

    Google Scholar 

  13. 13.

    Zhang ZF, Chen H, Li QL, Lei CZ, Zhang CL, Wang XZ, Wang JQ, Wang YM (2007) Polymorphisms of DGAT2 gene and its associations with several growth traits in Nanyang cattle. Hereditas (Beijing) 29(8):945–950

    Article  CAS  Google Scholar 

  14. 14.

    Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt chloroform extraction of DNA from blood and tissue. Trends Genet 5:391

    PubMed  CAS  Google Scholar 

  15. 15.

    Luo J, Wang ZY (1998) Practical production technology of meat goat. The Publishing House of Shaanxi Science and Technology, Xi’an

    Google Scholar 

  16. 16.

    Fang XT, Xu HX, Zhang CL, Chen H, Hu XC, Gao XY, Gu CW, Yue WP (2009) Polymorphism in BMP4 gene and its association with growth traits in goats. Mol Biol Rep 36:1339–1344

    PubMed  Article  CAS  Google Scholar 

  17. 17.

    Sun JJ, Zhang CL, Chen H, Fang XT, Jin QJ, Chen DX, Shi XY, Du Y (2010) Two novel SNPs in coding region of the caprine fat inducing transcript gene and their association with growth traits. Mol Biol Rep 37:485–490

    PubMed  Article  CAS  Google Scholar 

  18. 18.

    Chen ZQ, Sun JJ, Li ZJ, Lan XY, Zhang CL, Qu YJ, Liu YL, Fang XT, Lei CZ, Chen H (2010) Novel SNPs in the caprine stearoyl-CoA desaturase (SCD) and decorin (DCN) genes that are associated with growth traits in Chinese goat breeds. Mol Biol Rep. doi:10.1007/s11033-010-9982-8

  19. 19.

    Jin QJ, Sun JJ, Fang XT, Zhang CL, Yang L, Chen DX, Shi XY, Du Y, Lan XY, Chen H (2010) Molecular characterization and polymorphisms of the caprine Somatostatin (SST) and SST Receptor 1 (SSTR1) genes that are linked with growth traits. Mol Biol Rep. doi:10.1007/s11033-010-9983-7

  20. 20.

    Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada, Edmonton, Alberta, Canada

    Google Scholar 

  21. 21.

    Nei M, Li WH (1979) Mathematic model for studying genetic variation in terms of restriction endonucleaes. Proc Natl Acad Sci USA 76:5269–5273

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    Wang YZ, Xu YJ (2007) The application course for SAS software and statistics. Machinery Industry’s Publishing House

  23. 23.

    Bland JM, Altman DG (1995) Multiple significance tests: the Bonferroni method. BMJ 310:170

    PubMed  Article  CAS  Google Scholar 

  24. 24.

    Smith SJ, Cases S, Jensen DR, Chen HC, Sande E, Tow B, Sanan DA, Raber J, Eckel RH, Farese RV (2000) Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking DGAT. Nat Genet 25:87–90

    PubMed  Article  CAS  Google Scholar 

  25. 25.

    Wakimoto K, Chiba H, Michibata H, Seishima M, Kawasaki S, Okubo K, Mitsui H, Torii H, Imai Y (2003) A novel diacylglycerol acyltransferase (DGAT2) is decreased in human psoriatic skin and increased in diabetic mice. Biochem Biophys Res Commun 310(2):296–302

    PubMed  Article  CAS  Google Scholar 

  26. 26.

    Bucher H, Zapf J, Torresani T, Prader A, Froesch ER, Illig R (1983) Insulin-like growth factors I and II, prolactin, and insulin in 19 growth hormone-deficient children with excessive, normal, or decreased longitudinal growth after operation for craniopharyngioma. N Engl J Med 309(19):1142–1146

    PubMed  Article  CAS  Google Scholar 

  27. 27.

    Chen N, Liu L, Zhang Y, Ginsberg HN, Yu YH (2005) Whole-body insulin resistance in the absence of obesity in FVB mice with overexpression of DGAT 1 in adipose tissue. Diabetes 54(12):3379–3386

    PubMed  Article  CAS  Google Scholar 

  28. 28.

    Orland MD, Anwar K, Cromley D (2005) Acyl coenzyme a dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1. Biochim Biophys Acta 1737(1):76–82

    PubMed  CAS  Google Scholar 

  29. 29.

    Halloran FO, Bahar B, Buckley F, Sullivan OO, Sweeney T, Giblin L (2009) Characterisation of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds. Biochimie 91:68–75

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the Natural Science Foundation of Jiangsu Province (BK2008120), the Natural Science Fund for Colleges and Universities in Jiangsu Province (10KJD180003), Key Discipline Fund of Jiangsu Province (SYC0702), the Foundation of Xuzhou Normal University (08XLA09), the Research Fund for the Doctoral Program of Higher Education of China (200807120012).

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Correspondence to Hong Chen.

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Fang, X., Zhang, J., Xu, H. et al. Polymorphisms of diacylglycerol acyltransferase 2 gene and their relationship with growth traits in goats. Mol Biol Rep 39, 1801–1807 (2012). https://doi.org/10.1007/s11033-011-0921-0

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Keywords

  • Goat
  • DGAT-2 gene
  • PCR-SSCP
  • Genetic variation
  • Association analysis