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Molecular Biology Reports

, Volume 39, Issue 2, pp 1617–1626 | Cite as

A proteomic approach to analyze nitrogen- and cytokinin-responsive proteins in rice roots

  • Chengqiang Ding
  • Juan You
  • Shaohua Wang
  • Zhenghui Liu
  • Ganghua Li
  • Qiangsheng Wang
  • Yanfeng Ding
Article

Abstract

Nitrogen plays a central role in rice growth and development because it modulates a wide variety of processes, including cytokinin (CK) metabolism. CK-mediated signaling is also related to nitrogen metabolism. The functional relation between nitrogen and CK are extremely complex and unclear. In this study, a comparative proteomic analysis was carried out to analyze proteins regulated by nitrogen and CK in rice roots. Proteins extracted from rice roots are separated by two-dimensional polyacrylamide gel electrophoresis. Thirty-two protein spots that expressed similarly by nitrogen and CK treatments are selected for identification by mass spectrometry. Of these spots, 28 are successfully identified. These proteins were categorized into classes related to energy, metabolism, disease/defense, protein degradation, signal transduction, transposons, and unclear classification. Energy gives the largest functional category, suggesting that the glycolysis (two enzymes detected) and tricarboxylic acid cycle (six enzymes detected) are accurately regulated by nitrogen and CK, thus promoting the synthesis of amino acid. The identification of novel proteins provides new insights into the coordination of nitrogen and CK in rice. The possible role of these proteins is discussed.

Keywords

Cytokinin Nitrogen Proteomic Rice Root Two-dimensional gel electrophoresis 

Abbreviations

2-DE

Two-dimensional gel electrophoresis

ARR

Type-A Arabidopsis response regulators

IEF

Isoelectric focusing

iP

Isopentenyladenine

IPG

Immobilized pH gradient

iPR

iP riboside

MALDI-TOF/TOF

Matrix assisted laser desorption ionization time of flight

MS

Mass spectrometry

TCA

Tricarboxylic acid

Z

Zeatin

ZR

Z ribosid

Supplementary material

11033_2011_901_MOESM1_ESM.tif (1.7 mb)
Supplementary Fig. S1 All 2-DE gels of rice roots (TIFF 1762 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Chengqiang Ding
    • 1
  • Juan You
    • 1
  • Shaohua Wang
    • 1
  • Zhenghui Liu
    • 1
  • Ganghua Li
    • 1
  • Qiangsheng Wang
    • 1
  • Yanfeng Ding
    • 1
  1. 1.College of Agronomy, Nanjing Agricultural UniversityNanjingPeople’s Republic of China

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