Abstract
Autophagy is a membrane trafficking process involved in intracellular degradation and recycling in eukaryotic cells. DRAM2 (damage-regulated autophagy modulator 2) is a homologue of DRAM that regulates p53-mediated cell death. As its name implies, DRAM expression induces autophagy in a p53-dependent manner; however, the role of DRAM2 in autophagy is not clear. In this study, we report that DRAM2 expression contributes to autophagy induction. Overexpression of DRAM2 induces cytoplasmic GFP-LC3 punctuates, and increases the level of endogenous LC3-II. Moreover, the silencing of endogenous DRAM2 interferes with starvation-induced autophagy. Thus, we propose that DRAM2 as well as DRAM are involved in autophagy.
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Acknowledgments
We would particularly like to thank Kevin M. Ryan for providing the DRAM expression plasmid and T. Yoshimori for providing the GFP-LC3 plasmid. This study was supported by a National Research Foundation of Korea Grant funded by the Korean Government (2010-0009567 & 2009-0065887) and by the Mid-career Researcher Program (No. RO1-2008-000-20253-0).
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Yoon, JH., Her, S., Kim, M. et al. The expression of damage-regulated autophagy modulator 2 (DRAM2) contributes to autophagy induction. Mol Biol Rep 39, 1087–1093 (2012). https://doi.org/10.1007/s11033-011-0835-x
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DOI: https://doi.org/10.1007/s11033-011-0835-x