The expression of damage-regulated autophagy modulator 2 (DRAM2) contributes to autophagy induction
- 550 Downloads
Autophagy is a membrane trafficking process involved in intracellular degradation and recycling in eukaryotic cells. DRAM2 (damage-regulated autophagy modulator 2) is a homologue of DRAM that regulates p53-mediated cell death. As its name implies, DRAM expression induces autophagy in a p53-dependent manner; however, the role of DRAM2 in autophagy is not clear. In this study, we report that DRAM2 expression contributes to autophagy induction. Overexpression of DRAM2 induces cytoplasmic GFP-LC3 punctuates, and increases the level of endogenous LC3-II. Moreover, the silencing of endogenous DRAM2 interferes with starvation-induced autophagy. Thus, we propose that DRAM2 as well as DRAM are involved in autophagy.
KeywordsDRAM2 DRAM Autophagy Starvation LC3
We would particularly like to thank Kevin M. Ryan for providing the DRAM expression plasmid and T. Yoshimori for providing the GFP-LC3 plasmid. This study was supported by a National Research Foundation of Korea Grant funded by the Korean Government (2010-0009567 & 2009-0065887) and by the Mid-career Researcher Program (No. RO1-2008-000-20253-0).
- 14.Lorin S, Borges A, Ribeiro Dos Santos L, Souquere S, Pierron G, Ryan KM, Codogno P, Djavaheri-Mergny M (2009) c-Jun NH2-terminal kinase activation is essential for DRAM-dependent induction of autophagy and apoptosis in 2-methoxyestradiol-treated Ewing sarcoma cells. Cancer Res 69:6924–6931PubMedCrossRefGoogle Scholar