Molecular Biology Reports

, Volume 39, Issue 1, pp 227–231 | Cite as

Ethyl pyruvate reduces myocardial ischemia and reperfusion injury by inhibiting high mobility group box 1 protein in rats

  • Xiaorong Hu
  • Bo Cui
  • Xiaoya Zhou
  • Changwu Xu
  • Zhibing Lu
  • Hong Jiang


High mobility group box 1 protein (HMGB1) plays an important role in myocardial ischemia and reperfusion (I/R) injury. Ethyl pyruvate (EP), a potent reactive oxygen species scavenger, has been reported to inhibit myocardial apoptosis and reduce myocardial I/R injury. The aim of this study was to investigate the mechanism by which EP reduces myocardial I/R injury in rats. Anesthetized male rats were once treated with EP (50 mg/kg, i.p.) before ischemia, and then subjected to ischemia for 30 min followed by reperfusion for 4 h. Lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA), superoxide dismutase (SOD) activity and infarct size were measured. HMGB1 expression was assessed by immunoblotting. The results showed that pretreatment of EP (50 mg/kg) could significantly reduce the infarct size and the levels of LDH and CK after 4 h reperfusion (all P < 0.05). EP could also significantly inhibit the increase of the MDA level, the decrease of the SOD level (both P < 0.05). Meanwhile, EP could significantly inhibit the expression of HMGB1 induced by I/R. The present study suggested that ethyl pyruvate could attenuate myocardial I/R injury by inhibiting HMGB1 expression.


Ethyl pyruvate High mobility group box 1 protein Myocardial ischemia and reperfusion 



Ischemia and reperfusion


High mobility group box 1 protein


Tumor necrosis factor-α




Ethyl pyruvate


Sham operated control


Left anterior descending coronary artery


Lactate dehydrogenase


Creatine kinase




Superoxide dismutase


Glyceraldehyde-3-phosphate dehydrogenase


Reactive oxygen species


C-reactive protein



This study was partially supported by the Science Research Foundation of Wuhan University for MD candidate to Xiaorong Hu. We thank Ping Hu for her technical assistance.

Conflict of interest



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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Xiaorong Hu
    • 1
    • 2
  • Bo Cui
    • 1
    • 2
  • Xiaoya Zhou
    • 1
    • 2
  • Changwu Xu
    • 1
    • 2
  • Zhibing Lu
    • 1
    • 2
  • Hong Jiang
    • 1
    • 2
  1. 1.Department of CardiologyRenmin Hospital of Wuhan University and Cardiovascular Research Institute of Wuhan UniversityWuhanPeople’s Republic of China
  2. 2.Cardiovascular Research Institute of Wuhan UniversityWuhanPeople’s Republic of China

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