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Paraoxonase Arg 192 allele is an independent risk factor for three-vessel stenosis of coronary artery disease

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Abstract

The role of the paraoxonase (PON1) codon 192 polymorphism [glutamine (Q)/arginine (R)] in coronary artery disease (CAD) is controversial. The aim of the present study was to evaluate whether the PON1 gene polymorphism is an independent risk factor for severity of coronary artery disease in patients from west of Iran. The PON1-Arg-192 genotypes were detected by PCR-RFLP in 414 individuals undergoing their first coronary angiography. Patients were placed into one of two groups: CAD and control without CAD or diabetes. The frequency of PON1-Arg-192 allele was significantly higher in the CAD (23.4 vs. 16%, P = 0.032) than in the control group and there was a higher risk of developing CAD (OR = 1.6, P = 0.02). In addition, this difference remained significant after adjustment for without history of diabetes (OR = 1.47, P = 0.048), presence of normolipidemia and absence of history of blood pressure (OR = 1.4, P = 0.05). This result indicated PON1-Arg-192 allele is a risk factor of CAD also when correcting for conventional risk factors. We found a significant association between the PON1-Arg-192 genotype (QR + RR) and the extent of CAD in CAD patients and CAD subjects without diabetes, represented by the increased frequency of three-vessel disease with OR = 1.49, P = 0.046; χ2 = 3.82, P = 0.048 and OR = 1.46, P = 0.05; χ2 = 3.48, P = 0.051, respectively. The CAD patients carrying PON1-Arg-192 genotype (QR + RR) had lower plasma HDL-C level (P = 0.019) and higher plasma LDL-C(P = 0.01) and TG(P = 0.05). Our results indicated that PON1-Arg-192 allele can be important independent risk factor of CAD in a west population of Iran, with carriers of PON1-Arg-192 having an increased frequency of three-vessel disease and also having a distinct plasma lipids profile. Larger collaborative studies are needed to confirm these results.

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Acknowledgments

This work was supported by the Kermanshah University of Medical Sciences, Kermanshah, Iran (grant numbers 86049); All authors contributed equally to this study. This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.

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Authors and Affiliations

  1. Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Asad Vaisi-Raygani

  2. Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Daneshgah Avenue, P.O. Box 6714869914, Kermanshah, Iran

    Asad Vaisi-Raygani, Hori Ghaneialvar, Zohreh Rahimi, Ebrahim Shakiba & Reza Alibakhshi

  3. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Zohreh Rahimi

  4. Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran

    Haidar Tavilani

  5. Department of Molecular Sciences, Health Science Center, University of Tennessee, Knoxville, TN, USA

    Tayebeh Pourmotabbed

  6. Department of Operating Room, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Aliakbar Vaisi-Raygani

  7. Department of Toxicology, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Amir Kiani

  8. Department of Clinical Biochemistry, Tehran University of Medical Sciences, Tehran, Iran

    Mahdi Aminian

  9. Genetics Department, Case Western Reserve University, Cleveland, OH, USA

    Cynthia Bartels

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  1. Asad Vaisi-Raygani
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Correspondence to Asad Vaisi-Raygani.

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Vaisi-Raygani, A., Ghaneialvar, H., Rahimi, Z. et al. Paraoxonase Arg 192 allele is an independent risk factor for three-vessel stenosis of coronary artery disease. Mol Biol Rep 38, 5421–5428 (2011). https://doi.org/10.1007/s11033-011-0696-3

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