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Molecular Biology Reports

, Volume 38, Issue 8, pp 5179–5183 | Cite as

Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle

  • Zheng Rong Yuan
  • Jiao Li
  • Li Liu
  • Lu Pei Zhang
  • Li Min Zhang
  • Cui Chen
  • Xiao Jie Chen
  • Xue Gao
  • Jun Ya Li
  • Jin Bao Chen
  • Hui Jiang Gao
  • Shang Zhong Xu
Article

Abstract

The objective of the present study was to identify polymorphisms of the CACNA2D1 gene, and to analyze associations between these polymorphisms and mastitis in several cattle breeds. Through PCR–RFLP methods and DNA sequencing, an allelic variant corresponding to the A→G mutations and Aspartic (Asp) to Glycine (Gly) amino acid replacement at positions 526745 in the exon 25 of bovine CACNA2D1 gene could be detected. Two alleles, A and G, and three genotypes, AA, AG and GG were defined. Genetic character in the studied populations indicated that the A526745G loci of CACNA2D1 gene was moderate polymorphism and fitted with Hardy–Weinberg equilibrium (P > 0.05). The effects of CACNA2D1 polymorphisms on somatic cell score (SCS) were analyzed and significant association was found between A526745G and SCS. The mean of genotype GG was significantly lower than those of genotype AG and AA (P = 0.0469). Information provided in this research could be useful in further studies to determine the role of CACNA2D1 gene in the mastitis resistance.

Keywords

Cattle CACNA2D1 gene Somatic cell count Somatic cell score Mastitis 

Notes

Acknowledgments

This research was financially supported by the earmarked fund for Modern Agro-industry Technology Research System (No.nycytx-38) and the Eleventh “Five-Year” National Science and Technology Support Project (No. 2006BAD04A16 and No.2007BAD56B04).

References

  1. 1.
    Janzen JJ (1970) Economic losses resulting from mastitis: a review. J Dairy Sci 53:1151–1161PubMedCrossRefGoogle Scholar
  2. 2.
    Lescourret F, Coulon JB (1994) Modeling the impact of mastitis on milk production by dairy cows. J Dairy Sci 77:2289–2301PubMedCrossRefGoogle Scholar
  3. 3.
    Schukken YH, Lam TJGM, Barkema HW (1997) Biological basis for selection on udder health traits. Interbull Bull 15:27–33Google Scholar
  4. 4.
    Kossaibati MA, Hovi M, Esslemont RJ (1998) Incidence of clinical mastitis in dairy herds in England. Vet Rec 143:649–653PubMedGoogle Scholar
  5. 5.
    Nash DL, Rogers GW, Cooper JB, Hargrove GL, Keown JF (2003) Heritability of intramammary infections at first parturition and relationships with sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. J Dairy Sci 86:2684–2695PubMedCrossRefGoogle Scholar
  6. 6.
    Ruegg PL (2003) Investigation of mastitis problems on farms. Vet Clin North Am Food Anim Pract 19:47–73PubMedCrossRefGoogle Scholar
  7. 7.
    Zhang LP, Gan QF, Ma TH, Li HD, Wang XP, Li JY, Gao X, Chen JB, Ren HY, Xu SZ (2009) Toll-Like receptor 2 gene polymorphism and its relationship with SCS in dairy cattle. Anim Biotechnol 20:87–95PubMedCrossRefGoogle Scholar
  8. 8.
    Wang XP, Xu SZ, Gao X, Ren HY, Chen JB (2007) Genetic polymorphism of TLR4 gene and correlation with mastitis in cattle. J Genet Genomics 34:406–412PubMedCrossRefGoogle Scholar
  9. 9.
    Heringstad B, Klemetsdal G, Ruane J (2000) Selection for mastitis in dairy cattle: a review with focus on the situation of the Nordic countries. Livest Prod Sci 64:95–106CrossRefGoogle Scholar
  10. 10.
    Hansen M, Lund MS, Sorensen MK, Christensen LG (2002) Genetic parameters of dairy character, protein yield, clinical mastitis and other diseases in Danish Holstein cattle. J Dairy Sci 85:445–452PubMedCrossRefGoogle Scholar
  11. 11.
    Carle′n E, Strandberg E, Roth A (2004) Genetic parameters for clinical mastitis, somatic cell score and production in the first three lactations of Swedish Holstein cows. J Dairy Sci 87:3062–3070CrossRefGoogle Scholar
  12. 12.
    Lund MS, Jensen J, Petersen PH (1999) Estimation of genetic and phenotypic parameters for clinical mastitis, somatic cell production deviance and protein yield in dairy cattle using Gibbs sampling. J Dairy Sci 82:1045–1051PubMedCrossRefGoogle Scholar
  13. 13.
    Heringstad B, Gianola D, Chang YM, Odegard J, Klemetsdal G (2006) Genetic associations between clinical mastitis and somatic cell score in early first-lactation cows. J Dairy Sci 89:2236–2244PubMedCrossRefGoogle Scholar
  14. 14.
    Rupp R, Boichard D (1999) Genetic parameters for clinical mastitis, somatic cell score, production, udder type traits, and milking ease in first lactation Holsteins. J Dairy Sci 82:2198–2204PubMedCrossRefGoogle Scholar
  15. 15.
    Holmberg M, Andersson-Eklund L (2004) Quantitative trait loci affecting health traits in Swedish dairy cattle. J Dairy Sci 87:2653–2659PubMedCrossRefGoogle Scholar
  16. 16.
    Hou GY, Yuan ZR, Gao X, Jun YL, Gao HJ, Chen JB, Xu SZ (2010) Genetic polymorphisms of CACNA2D1 gene and their association with carcass and meat quality traits in cattle. Biochem Genet 48:751–759PubMedCrossRefGoogle Scholar
  17. 17.
    Yuan ZR, Xu SZ (2011) Novel SNPs of the bovine CACNA2D1 gene and their association with carcass and meat quality traits. Mol Biol Rep 38:365–370PubMedCrossRefGoogle Scholar
  18. 18.
    Liu H, Tian W, Zan L, Wang H, Cui H (2010) Mutations of MC4R gene and its association with economic traits in Qinchuan cattle. Mol Biol Rep 37(1):535–540PubMedCrossRefGoogle Scholar
  19. 19.
    Zhang CL, Wang YH, Chen H, Lan XY, Lei CZ, Fang XT (2009) Association between variants in the 5′-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle. Mol Biol Rep 36(7):1839–1843PubMedCrossRefGoogle Scholar
  20. 20.
    Xu ZY, Xiong YZ, Lei MG, Li FE, Zuo B (2009) Genetic polymorphisms and preliminary association analysis with production traits of the porcine SLC27A4 gene. Mol Biol Rep 36(6):1427–1432PubMedCrossRefGoogle Scholar
  21. 21.
    te Pas MF, Soumillion A, Harders FL, Verburg FJ, van den Bosch TJ, Galesloot P et al (1999) Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness, and lean weight of pigs. J Anim Sci 77(9):2352–2356PubMedGoogle Scholar
  22. 22.
    Xu X, Qiu H, Du ZQ, Fan B, Rothschild MF, Yuan F, Liu B (2010) Porcine CSRP3: polymorphism and association analyses with meat quality traits and comparative analyses with CSRP1 and CSRP2. Mol Biol Rep 37(1):451–459PubMedCrossRefGoogle Scholar
  23. 23.
    Zhou G, Dudgeon C, Li M, Cao Y, Zhang L, Jin H (2010) Molecular cloning of the HGD gene and association of SNPs with meat quality traits in Chinese red cattle. Mol Biol Rep 37(1):603–611PubMedCrossRefGoogle Scholar
  24. 24.
    Fontanesi L, Scotti E, Buttazzoni L, Dall’Olio S, Bagnato A, Lo Fiego DP, Davoli R, Russo V (2010) Confirmed association between a single nucleotide polymorphism in the FTO gene and obesity-related traits in heavy pigs. Mol Biol Rep 37(1):461–466PubMedCrossRefGoogle Scholar
  25. 25.
    Davies A, Hendrich J, Minh ATV, Wratten J, Douglas L, Dolphin AC (2007) Functional biology of the α2/δ subunits of voltage-gated calcium channels. Trends Pharmacol Sci 28:220–228PubMedCrossRefGoogle Scholar
  26. 26.
    Buitkamp J, Ewald D, Masabanda J, Bishop MD, Fries R (2003) FISH and RH mapping of the bovine alpha (2)/delta calcium channel subunit gene (CACNA2D1). Anim Genet 34:302–318CrossRefGoogle Scholar
  27. 27.
    Zhang Q, Boichard D, Hoeschele I, Ernst C, Eggen A, Murkve B, Pfister-Genskow M, Witte LA, Grignola FE, Uimari P, Thaller G, Bishop MD (1998) Mapping quantitative trait loci for milk production and health of dairy cattlein a large outbred pedigree. Genetics 149:1959–1973PubMedGoogle Scholar
  28. 28.
    Rupp R, Boichard D (2003) Genetics of resistance to mastitis in dairy cattle. Vet Res 34:671–688PubMedCrossRefGoogle Scholar
  29. 29.
    Daetwyler HD, Schenkel FS, Sargolzaei M, Robinson JAB (2008) Genome scans in dairy cattle using a dense SNP map. J Dairy Sci 91:3225–3236PubMedCrossRefGoogle Scholar
  30. 30.
    Longeri M, Polli M, Strillacci MG, Samore AB, Zanotti M (2006) Short communication: quantitative trait loci affecting the somatic cell score onchromosomes 4 and 26 in Italian Holstein cattle. J Dairy Sci 89:3175–3177PubMedCrossRefGoogle Scholar
  31. 31.
    Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissues. Trends Genet 5:391PubMedGoogle Scholar
  32. 32.
    Shook GE (1982) Approaches to summarizing somatic cell counts which improve interpretability. Proceedings of 21st annual meeting, National Mastitis Council, Arlington, 1982 pp.150–166Google Scholar
  33. 33.
    Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Edmonton, Alberta. (http://www.ualberta.ca/*fyeh/info.html)
  34. 34.
    SAS Institute (1999) Statistical Analysis Systems User’s Guide (Version 80). SAS Institute, Inc, Cary, NCGoogle Scholar
  35. 35.
    Park YH, Joo YS, Park JY, Moon JS, Kim SH, Kwon NH, Ahn JS, Davis WC (2004) Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows. J Vet Sci 5:29–39PubMedGoogle Scholar
  36. 36.
    Noguera JL, Varona L, Go′mez-Raya L, Sa′nchez A, Babot D, Estany J, Messer LA, Rothschild M, Pe′rez-Enciso M (2003) Estrogen receptor polymorphism in Landrace pigs and its association with litter size performance. Livest Prod Sci 2:53–59CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zheng Rong Yuan
    • 1
  • Jiao Li
    • 1
  • Li Liu
    • 1
  • Lu Pei Zhang
    • 1
  • Li Min Zhang
    • 1
  • Cui Chen
    • 1
  • Xiao Jie Chen
    • 1
  • Xue Gao
    • 1
  • Jun Ya Li
    • 1
  • Jin Bao Chen
    • 1
  • Hui Jiang Gao
    • 1
  • Shang Zhong Xu
    • 1
  1. 1.Laboratory of Molecular Biology and Bovine Breeding, The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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