Skip to main content
SpringerLink
Log in

Genetic analysis of the 10 ChrX STRs loci in Chinese Han nationality from Guangdong province

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

This study is to explore the polymorphic nature of X-Chromosome short tandem repeats (ChrX STRs) loci, and to determine its application in kinship tests for forensic cases. A new fluorescent multiplex PCR that simultaneously amplifies the 10 ChX STRs loci in the same PCR reaction had been set up. DXS7132, DXS981, DXS6801, DXS6809, DXS6789, DXS7424, DXS101, DXS7133, GATA165B12 and GATA31E08 were analyzed in a sample of 511 (399 males and 112 females) unrelated individuals from Guangdong Han nationality in China. One hundred and one alleles were observed in all the loci. Here, we investigated the allele frequencies and mutation rates of the ten loci, and then made the comparison of allele frequencies distribution among different populations. The results show the ten loci in the multiplex systems may provide high polymorphism information for kinship testing and relationship investigations, and it is necessary to gain allele frequency and mutation rate of different population for forensic application.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Liu QL, Lv DJ, Wu XL, Sun HY, Wu XY, Lu HL (2008) Development of a five ChX STRs loci typing system. Int J Legal Med 122:261–265

    Article  PubMed  Google Scholar 

  2. Wu WW, Hao HL, Liu QL, Su YJ, Zheng XT, Lu DJ (2009) Allele frequencies of seven X-linked STR loci in Chinese Han population from Zhejiang Province. Forensic Sci Int Genet 4:e41–e42

    Article  PubMed  CAS  Google Scholar 

  3. Nadeem A, Babar ME, Hussain M, Tahir MA (2009) Development of pentaplex PCR and genetic analysis of X chromosomal STRs in Punjabi population of Pakistan. Mol Biol Rep 36:1671–1675

    Article  PubMed  CAS  Google Scholar 

  4. Sim JE, Lee HY, Yang WI, Shin KJ (2010) Population genetic study of four closely-linked X-STR trios in Koreans. Mol Biol Rep 37:333–337

    Article  PubMed  CAS  Google Scholar 

  5. Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801-DXS6809-DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369

    Article  PubMed  Google Scholar 

  6. Tariq MA, Sabir MF, Riazuddin SA, Riazuddin S (2009) Haplotype analysis of two X-chromosome STR clusters in the Pakistani population. Int J Legal Med 123:85–87

    Article  PubMed  Google Scholar 

  7. Hwa HL, Chang YY, Lee JC, Yin HY, Chen YH, Tseng LH, Su YN, Ko TM (2009) Thirteen X-chromosomal short tandem repeat loci multiplex data from Taiwanese. Int J Legal Med 123:263–269

    Article  PubMed  Google Scholar 

  8. Walsh PS, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513

    PubMed  CAS  Google Scholar 

  9. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformatics Online 1:47–50

    CAS  Google Scholar 

  10. Botstein RW D, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:324–331

    Google Scholar 

  11. Desmarais D, Zhong Y, Chakraborty R, Perreault C, Busque L (1998) Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA). J Forensic Sci 43:1046–1049

    PubMed  CAS  Google Scholar 

  12. Szibor R, Hering S, Edelmann J (2006) A new Web site compiling forensic chromosome X research is now online. Int J Legal Med 120:252–254

    Article  PubMed  Google Scholar 

  13. Liu QB, Li SB (2006) Patterns of genetic polymorphism at the 10 X-chromosome STR loci in Mongol population. Forensic Sci Int 158:76–79

    Article  PubMed  CAS  Google Scholar 

  14. Kang LL, Li SB (2006) X-chromosome STR polymorphism of Luoba Ethnic Group living in Tibet (SW China). Forensic Sci Int 156:88–90

    Article  PubMed  CAS  Google Scholar 

  15. Bekada A, Benhamamouch S, Boudjema A, Fodil M, Menegon S, Torre C, Robino C (2010) Analysis of 21 X-chromosomal STRs in an Algerian population sample. Int J Legal Med 124:287–294

    Article  PubMed  Google Scholar 

  16. Turrina S, Atzei R, Filippini G, De Leo D (2007) Development and forensic validation of a new multiplex PCR assay with 12 X-chromosomal short tandem repeats. Forensic Sci Int Genet 1:201–204

    Article  PubMed  Google Scholar 

  17. Edelmann J, Hering S, Kuhlisch E, Szibor R (2002) Validation of the STR DXS7424 and the linkage situation on the X-chromosome. Forensic Sci Int 125:217–222

    Article  PubMed  CAS  Google Scholar 

  18. Brinkmann B, Junge A, Meyer E, Wiegand P (1998) Population genetic diversity in relation to microsatellite heterogeneity. Hum Mutat 11:135–144

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the Guangdong province innovation experiment program for university students, China(1055810103).

Author information

Authors and Affiliations

  1. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, 74, Zhongshan 2nd Road, Guangzhou, 510080, People’s Republic of China

    Qiu-Ling Liu, De-Jian Lu, Ye-Fei Chen & Hu Zhao

  2. Department of Criminal Investigation, Zhejiang Provincial Public Security Bureau, 66, Minsheng Road, 310009, Hangzhou, China

    Wei-Wei Wu & Hong-Lei Hao

Authors
  1. Qiu-Ling Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. De-Jian Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei-Wei Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hong-Lei Hao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ye-Fei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hu Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hu Zhao.

Additional information

D.-J. Lu is the Co-first author

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, QL., Lu, DJ., Wu, WW. et al. Genetic analysis of the 10 ChrX STRs loci in Chinese Han nationality from Guangdong province. Mol Biol Rep 38, 4879–4883 (2011). https://doi.org/10.1007/s11033-010-0629-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-010-0629-6

Keywords

Search

Navigation