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Characterization and expression analysis of the myeloid differentiation factor 88 (MyD88) in rock bream Oplegnathus fasciatus

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Abstract

Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein able to activate nuclear factor-kappa B (NF-κB) through interactions with interleukin-1 receptor (IL-1R) and the Toll-like receptors (TLRs), with the exception of TLR3. Here, we describe the identification of MyD88 from the rock bream fish Oplegnathus fasciatus and its characterization based on GS-FLX™ sequencing. The cDNA of rock bream MyD88 was found to be composed of 1626 bp, with an 867 bp open reading frame that encodes 288 amino acids. The deduced amino acid sequence of MyD88 possessed both a conserved death domain at the amino terminus and a typical Toll-IL-1 receptor (TIR) domain at the carboxyl terminus, similar to that found in other fishes, amphibians, avians, mammals and invertebrates. The mRNA expression pattern of MyD88 in healthy and bacterially challenged rock bream were examined using quantitative real-time polymerase chain reaction (qRT-PCR). MyD88 transcripts were found to be strongly expressed in blood, gill, liver, spleen, head kidney and kidney, moderately expressed in skin, brain and intestine, and weakly expressed in muscle. Expression levels of MyD88 in blood, spleen and head kidney were dramatically up-regulated upon exposure to LPS and the Gram-negative bacteria Edwardsiella tarda, suggesting that MyD88 plays an important role in rock bream defenses against bacterial infection.

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Acknowledgments

This research was supported by a grant from the National Fisheries Research and Development Institute (NFRDI, RP-2010-BT-030), Republic of Korea.

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Authors and Affiliations

  1. Department of Life Sciences, College of Natural Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, 690-756, Republic of Korea

    Ilson Whang & Se-Jae Kim

  2. Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, 690-756, Republic of Korea

    Youngdeuk Lee, Hyowon Kim & Jehee Lee

  3. Department of Aqualife Medicine, Chonnam National University, Chonnam, 550-749, Republic of Korea

    Sung-Ju Jung & Myung-Joo Oh

  4. Division of Marine Environment and Bioscience, Korea Maritime University, Busan, 606-791, Republic of Korea

    Cheol Young Choi

  5. Bioindustry Technology Research Center and AI Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 580-185, Republic of Korea

    Woo Song Lee

  6. Marine and Environmental Institute, Jeju National University, Jeju-si, Jeju Special Self-Governing Province, 690-814, Republic of Korea

    Jehee Lee

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  1. Ilson Whang
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Correspondence to Se-Jae Kim or Jehee Lee.

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Whang, I., Lee, Y., Kim, H. et al. Characterization and expression analysis of the myeloid differentiation factor 88 (MyD88) in rock bream Oplegnathus fasciatus . Mol Biol Rep 38, 3911–3920 (2011). https://doi.org/10.1007/s11033-010-0507-2

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  • DOI: https://doi.org/10.1007/s11033-010-0507-2

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