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Molecular Biology Reports

, Volume 38, Issue 6, pp 3665–3670 | Cite as

Molecular cloning and characterization of caprine calpastatin gene

  • Dai Hua Wang
  • Gang Yi Xu
  • Deng Jun Wu
  • Cheng Li Zheng
Article
  • 173 Downloads

Abstract

Calpastatin (CAST) is an important gene for meat quality traits in livestock and poultry. The cDNA of caprine CAST gene was amplified for the first time using RACE-PCR. Results showed the full-length cDNA of caprine CAST gene (Accession no. GU944861) was 2435 base pair (bp) and contained a 2187 bp open reading frame encoding a protein with 728 amino acid residues. Bioinformatic analysis indicated that caprine CAST cDNA was 89.8–95.4, 83.5–92.2, 72.8–81.8 and 69.8–73.5% identical to sheep, cattle, pig and human CAST cDNA. It was predicted that caprine CAST contained four conserved domains with 42 serine phosphorylation loci, 18 threonine phosphorylation loci, 1 tyrosine phosphorylation locus and 5 specific PKC phosphorylation loci. This work provided an important experimental basis for further research on the function of CAST in goat.

Keywords

Cloning Calpastatin gene Goat RACE 

Notes

Acknowledgments

This work was financially supported by the National Modern Industry Technology System on Sheep and Goat for Meat (NYCYTX-39) and the Projects of Selecting New Breed on Livestock in Sichuan Province (01NG002-06).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Dai Hua Wang
    • 1
  • Gang Yi Xu
    • 1
  • Deng Jun Wu
    • 1
  • Cheng Li Zheng
    • 1
  1. 1.College of Animal Science & TechnologySichuan Agricultural UniversityYa’anPeople’s Republic of China

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