Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H2O2-induced PC12 cells
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In this study, the effect of aucubin on H2O2-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H2O2-induced PC12 cells, H2O2-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3 leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression, but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. These results indicated that aucubin can obstruct H2O2-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation.
KeywordsAucubin Hydrogen peroxide Apoptosis PC12 cells Bcl-2 family
Financial support for this research from the Scientific Research Foundation of Suzhou University for Doctor (2009jb05), the Natural Science Research Project of Education Office of Anhui Province (KJ2010B225) is gratefully acknowledged. The authors thank Dr. Leigh Campell from GNC Bioferm Inc. Canada Agriculture and Dr. Katherine Jakober from Agri-Food Canada Lethbridge Research Centre for revising a manuscript before publication.