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Molecular Biology Reports

, Volume 38, Issue 5, pp 3561–3567 | Cite as

Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H2O2-induced PC12 cells

  • Hong Yu Xue
  • Dong Yan Niu
  • Gui Zhen Gao
  • Qiu Ye Lin
  • Li Ji Jin
  • Yong Ping Xu
Article

Abstract

In this study, the effect of aucubin on H2O2-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H2O2-induced PC12 cells, H2O2-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3 leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression, but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. These results indicated that aucubin can obstruct H2O2-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation.

Keywords

Aucubin Hydrogen peroxide Apoptosis PC12 cells Bcl-2 family 

Notes

Acknowledgments

Financial support for this research from the Scientific Research Foundation of Suzhou University for Doctor (2009jb05), the Natural Science Research Project of Education Office of Anhui Province (KJ2010B225) is gratefully acknowledged. The authors thank Dr. Leigh Campell from GNC Bioferm Inc. Canada Agriculture and Dr. Katherine Jakober from Agri-Food Canada Lethbridge Research Centre for revising a manuscript before publication.

References

  1. 1.
    Topuridze ML, Kipiani VA, Pavliashvili NS, Kipiani NV, Petriashvili TG (2007) Molecular mechanisms of apoptosis. Georgian Med News 150:38–45PubMedGoogle Scholar
  2. 2.
    Lee SC, Pervaiz S (2007) Apoptosis in the pathophysiology of diabetes mellitus. Int J Biochem Cell Biol 39:497–504PubMedCrossRefGoogle Scholar
  3. 3.
    Eom KS, Hong JM, Youn MJ, So HS, Park R, Kim JM, Kim TY (2008) Berberine induces G1 arrest and apoptosis in human glioblastoma T98G cells through mitochondrial/caspases pathway. Biol Pharm Bull 31(4):558–562PubMedCrossRefGoogle Scholar
  4. 4.
    Sun XM, Bratton SB, Butterworth M, MacFarlane M, Cohen GM (2002) Bcl-2 and Bcl-xL inhibit CD95-mediated apoptosis by preventing mitochondrial release of Smac/DIABLO and subsequent inactivation of X-linked inhibitor-of-apoptosis protein. J Biol Chem 277:11345–11351PubMedCrossRefGoogle Scholar
  5. 5.
    Cheng XR, Zhang L, Hu JJ, Sun L, Du GH (2007) Neuroprotective effects of tetramethylpyrazine on hydrogen peroxide-induced apoptosis in PC12 cells. Cell Biol Int 31(5):438–443PubMedCrossRefGoogle Scholar
  6. 6.
    Magherini F, Tani C, Gamberi T, Caselli A, Bianchi L, Bini L, Modesti A (2007) Protein expression profiles in Saccharomyces cerevisiae during apoptosis induced by H2O2. Proteomics 7(9):1434–1445PubMedCrossRefGoogle Scholar
  7. 7.
    Chaube SK, Prasad PV, Thakur SC, Shrivastav TG (2005) Hydrogen peroxide modulates meiotic cell cycle and induces morphological features characteristic of apoptosis in rat oocytes cultured in vitro. Apoptosis 10(4):863–874PubMedCrossRefGoogle Scholar
  8. 8.
    Chandra J, Samali A, Orrenius S (2000) Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med 29:323–333PubMedCrossRefGoogle Scholar
  9. 9.
    Bernini R, Iavarone C, Trogolo C (1984) 1-O-β-d-glucopyranosyleucommiol, an iridoid glucoside from Aucuba japonica. Phytochemistry 23:1431–1433CrossRefGoogle Scholar
  10. 10.
    Bianco A, Iavarone C, Trogolo C (1974) Structure of eucommiol, a new cyclopentenoid-tetraol from Eucommia ulmoides. Tetrahedron 30:4117–4121CrossRefGoogle Scholar
  11. 11.
    Pailer M, Haschke-Hofmeister E (1969) Inhaltsttoffe aus Plantago major. Planta Med 17:139–145PubMedCrossRefGoogle Scholar
  12. 12.
    Suomi J, Sirén H, Wiedmer SK, Riekkola ML (2001) Isolation of aucubin and catalpol from Melitaea cinxia larvae and quantification by micellar electrokinetic capillary chromatography. Anal Chim Acta 429:91–99CrossRefGoogle Scholar
  13. 13.
    Chang IM, Ryu JC, Park YC, Yun HS, Yang KH (1983) Protective activities of aucubin against carbon tetrachloride-induced liver damage in mice. Drug Chem Toxicol 6:443–453PubMedCrossRefGoogle Scholar
  14. 14.
    Davini E, Iavarone C, Trogolo C, Aureli P, Pasolini B (1986) The quantitative isolation and antimicrobial activity of the aglycome of aucubin. Phytochemistry 25:2420–2422CrossRefGoogle Scholar
  15. 15.
    Chang IM (1998) Liver protective activities of aucubin derived from traditional oriental medicine. Res Commun Mol Pathol Pharmacol 102:189–204PubMedGoogle Scholar
  16. 16.
    Wolter KG, Hsu YT, Smith CL, Nechushtan A, Xi XG, Youle RJ (1997) Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 139:1281–1292PubMedCrossRefGoogle Scholar
  17. 17.
    Chang HJ, Yoon G, Park JS, Kim MH, Baek MK, Kim NH, Shin BA, Ahn BW, Cheon SH, Jung YD (2007) Induction of apoptosis by the licochalcone E in endothelial cells via modulation of NF-kappaB and Bcl-2 Family. Biol Pharm Bull 30(12):2290–2293PubMedCrossRefGoogle Scholar
  18. 18.
    Tsujimoto Y, Croce CM (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci USA 83:5214–5218PubMedCrossRefGoogle Scholar
  19. 19.
    Thomas BF, Gilliam AF, Burch DF, Roche MJ, Seltzman HH (1998) Comparative receptor binding analyses of cannabinoid agonists and antagonists. J Pharmacol Exp Ther 285:285–292PubMedGoogle Scholar
  20. 20.
    Kang YJ, Jang M, Park YK, Kang S, Bae KH, Cho S, Lee CK, Park BC, Chi SW, Park SG (2010) Molecular interaction between HAX-1 and XIAP inhibits apoptosis. Biochem Biophys Res Commun 393(4):794–799PubMedCrossRefGoogle Scholar
  21. 21.
    Guo R, Ren J (2010) Alcohol dehydrogenase accentuates ethanol-induced myocardial dysfunction and mitochondrial damage in mice: role of mitochondrial death pathway. PLoS One 5(1):e8757PubMedCrossRefGoogle Scholar
  22. 22.
    Zecchin KG, Seidinger AL, Chiaratti MR, Degasperi GR, Meirelles FV, Castilho RF, Vercesi AE (2007) High Bcl-2/Bax ratio in Walker tumor cells protects mitochondria but does not prevent H2O2-induced apoptosis via calcineurin pathways. J Bioenerg Biomembr 39(2):186–194PubMedCrossRefGoogle Scholar
  23. 23.
    Park YH, Son IH, Kim B, Lyu YS, Moon HI, Kang HW (2009) Poria cocos water extract (PCW) protects PC12 neuronal cells from beta-amyloid-induced cell death through antioxidant and antiapoptotic functions. Pharmazie 64(11):760–764PubMedGoogle Scholar
  24. 24.
    Thangapazham RL, Singh AK, Seth P, Misra N, Mathad VT, Raj K, Maheshwari RK (2008) Shikonin analogue (SA) 93/637 induces apoptosis by activation of caspase-3 in U937 cells. Front Biosci 13:561–568PubMedCrossRefGoogle Scholar
  25. 25.
    Miñano A, Caballero-Benítez A, Lluch M, Morán J, Rodríguez-Alvarez J (2008) C2-ceramide mediates cerebellar granule cells apoptosis by activation of caspases-2, -9, and -3. J Neurosci Res 86(8):1734–1747PubMedCrossRefGoogle Scholar
  26. 26.
    Sommer M, Wolf G (2007) Rosiglitazone increases PPAR gamma in renal tubular epithelial cells and protects against damage by hydrogen peroxide. Am J Nephrol 27(4):425–434PubMedCrossRefGoogle Scholar
  27. 27.
    Jiang B, Liu JH, Bao YM, An LJ (2004) Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase cascade. Toxicon 43(1):53–59PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Chemistry and Life ScienceSuzhou UniversitySuzhouPeople’s Republic of China
  2. 2.Department of Bioscience and BiotechnologyDalian University of TechnologyDalianPeople’s Republic of China
  3. 3.Dalian SEM Bioengineer and Biotech LtdDalianPeople’s Republic of China
  4. 4.Faculty of Animal Science and TechnologyYunnan Agricultural UniversityKunmingPeople’s Republic of China

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