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Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes

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Abstract

Cultivated peanut possesses an extremely narrow genetic basis. Polymorphism is considerably difficult to identify with the use of conventional biochemical and molecular tools. For the purpose of obtaining considerable DNA polymorphisms and fingerprinting cultivated peanut genotypes in a convenient manner, start codon targeted polymorphism technique was used to study genetic diversity and relatedness among 20 accessions of four major botanical varieties of peanut. Of 36 primers screened, 18 primers could produce unambiguous and reproducible bands. All 18 primers generated a total of 157 fragments, with a mean of 8.72 ranging from 4 to 17 per primer. Of 157 bands, 60 (38.22%) were polymorphic. One to seven polymorphic bands were amplified per primer, with 3.33 polymorphic bands on average. Polymorphism per primer ranged from 14.29 to 66.67%, with an average of 36.76%. The results revealed that not all accessions of the same variety were grouped together and high genetic similarity was detected among the tested genotypes based on cluster analysis and genetic distance analysis, respectively. Further, accession-specific markers were observed in several accessions. All these results demonstrated the following: (1) start codon targeted polymorphism technique can be utilized to identify DNA polymorphisms and fingerprint cultivars in domesticated peanut, and (2) it possesses considerable potential for studying genetic diversity and relationships among peanut accessions.

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Acknowledgments

We are grateful to Dr. J.M. Wu for the technical assistance he has provided for this study. We wish to extend our gratitude to Dr. B.C.Y. Collard (International Rice Research Institute, IRRI), Dr. Y.W. Wei and Dr. W.D. Shi for their valuable discussions in the process of writing the manuscript. This research was supported by the earmarked fund for Modern Agro-Industry Technology Research System and grants from the National Natural Science Foundation of China (No. 30660094) and Guangxi Science Fund (Guikezi 0832088).

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Authors and Affiliations

  1. Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China

    Faqian Xiong, Ruichun Zhong, Zhuqiang Han, Jing Jiang, Liangqiong He & Ronghua Tang

  2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China

    Faqian Xiong, Zhuqiang Han, Jing Jiang & Ronghua Tang

  3. Fujian Province Key Lab of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China

    Weijian Zhuang

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  1. Faqian Xiong
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Correspondence to Weijian Zhuang or Ronghua Tang.

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Xiong, F., Zhong, R., Han, Z. et al. Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol Biol Rep 38, 3487–3494 (2011). https://doi.org/10.1007/s11033-010-0459-6

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  • DOI: https://doi.org/10.1007/s11033-010-0459-6

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