Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes
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Cultivated peanut possesses an extremely narrow genetic basis. Polymorphism is considerably difficult to identify with the use of conventional biochemical and molecular tools. For the purpose of obtaining considerable DNA polymorphisms and fingerprinting cultivated peanut genotypes in a convenient manner, start codon targeted polymorphism technique was used to study genetic diversity and relatedness among 20 accessions of four major botanical varieties of peanut. Of 36 primers screened, 18 primers could produce unambiguous and reproducible bands. All 18 primers generated a total of 157 fragments, with a mean of 8.72 ranging from 4 to 17 per primer. Of 157 bands, 60 (38.22%) were polymorphic. One to seven polymorphic bands were amplified per primer, with 3.33 polymorphic bands on average. Polymorphism per primer ranged from 14.29 to 66.67%, with an average of 36.76%. The results revealed that not all accessions of the same variety were grouped together and high genetic similarity was detected among the tested genotypes based on cluster analysis and genetic distance analysis, respectively. Further, accession-specific markers were observed in several accessions. All these results demonstrated the following: (1) start codon targeted polymorphism technique can be utilized to identify DNA polymorphisms and fingerprint cultivars in domesticated peanut, and (2) it possesses considerable potential for studying genetic diversity and relationships among peanut accessions.
KeywordsStart codon targeted polymorphism (SCoT) Peanut botanical variety Genetic diversity Functional molecular markers
We are grateful to Dr. J.M. Wu for the technical assistance he has provided for this study. We wish to extend our gratitude to Dr. B.C.Y. Collard (International Rice Research Institute, IRRI), Dr. Y.W. Wei and Dr. W.D. Shi for their valuable discussions in the process of writing the manuscript. This research was supported by the earmarked fund for Modern Agro-Industry Technology Research System and grants from the National Natural Science Foundation of China (No. 30660094) and Guangxi Science Fund (Guikezi 0832088).
- 3.Tang RH, Zhuang WJ, Gao GQ, He LQ, Han ZQ, Shan SH, Jiang J, Li YR (2008) Phylogenetic relationships in genus Arachis based on SSR and AFLP markers. Agric Sci China 7(4):405–414Google Scholar
- 14.Raina SN, Rani V, Kojima T, Ogihara Y, Singh KP, Devarumath RM (2001) RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species. Genome 44(5):763–772PubMedGoogle Scholar
- 20.Han ZQ, Gao GQ, Wei PX, Tang RH, Zhong RC (2004) Analysis of DNA polymorphism and genetic relationships in cultivated peanut (Arachis hypogaea L.) using microsatellite markers. Acta Agronomica Sinica 30(11):1097–1101Google Scholar
- 23.Moretzsohn MC, Barbosa AVG, Alves-Freitas DMT, Teixeira C, Leal-Bertioli SCM, Guimaraes PM, Pereira RW, Lopes CR, Cavallari MM, Valls JFM, Bertioli DJ, Gimenes MA (2009) A linkage map for the B-genome of Arachis (Fabaceae) and its synteny to the A-genome. BMC Plant Biol 9:40. doi: 10.1186/1471-2229-9-40 PubMedCrossRefGoogle Scholar
- 24.Foncéka D, Hodo-Abalo T, Rivallan R, Faye I, Sall MN, Ndoye O, Fávero AP, Bertioli DJ, Glaszmann JC, Courtois B, Rami JF (2009) Genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid. BMC Plant Biol 9:103. doi: 10.1186/1471-2229-9-103 PubMedCrossRefGoogle Scholar
- 25.Hong YB, Liang XQ, Chen XP, Liu HY, Zhou GY, Li SX, Wen SJ (2008) Construction of genetic linkage map based on SSR markers in peanut (Arachis hypogaea L.). Agric Sci China 7(8):915–921Google Scholar