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Molecular Biology Reports

, Volume 38, Issue 4, pp 2257–2262 | Cite as

Binding Citrus flavanones to human serum albumin: effect of structure on affinity

  • Hui Cao
  • Longsheng Chen
  • Jianbo Xiao
Article

Abstract

Much of the bioactivities of Citrus flavanones significantly appear to impact blood and microvascular endothelial cells. It is essential to investigate the interaction between Citrus flavanones and serum albumin to verify the effect of flavanone structures on the distribution and transportation in blood. The interactions between flavonoids and proteins have attracted great interest among researchers. The work in here mainly concerns about the binding interaction between Citrus flavanones and human serum albumin (HSA) in vitro. The methoxylation of tangeretin improved the affinity for HSA by 100 times. The 2,3-double bond in conjugation with a 4-oxo group plays an important role for the affinity for HSA. The affinity of apigenin for HSA is about 10,000-times higher than that of naringenin. It was found that the hydroxylation on position 3′ of flavonol significantly improves the binding affinity for HSA. The affinity of quercetin (3′, 4′) for HSA is about 100-times higher than that of kaempferol (4′). The hydroxylation on position 3′ of flavone slightly improves the binding affinity for HSA. The affinity of luteolin for HSA is about 1.38-times higher than that of apigenin. The values of log10(Ka) are proportional to the number of binding sites (n), which confirms the method used here is suitable to study the interaction between Citrus flavanones and HSA.

Keywords

Citrus flavanones Affinity HSA Naringenin Naringin Narirutin Nobiletin Tangeretin 

Notes

Acknowledgments

The authors are grateful for the financial support sponsored by Natural Science Foundation of Shanghai (10ZR1421700), “Chen Guang” project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (09CG46), Leading Academic Discipline Project of Shanghai Municipal Education Commission (J50401) and Innovation Program of Shanghai Municipal Education Commission (10YZ68).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.School of Chemistry and Chemical EngineeringNantong UniversityNantongPeople’s Republic of China
  2. 2.Application Research LaboratoryAnhui Academy of Science & TechnologyHefeiPeople’s Republic of China
  3. 3.College of Life & Environment ScienceShanghai Normal UniversityShanghaiPeople’s Republic of China

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