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Molecular Biology Reports

, Volume 38, Issue 3, pp 1935–1940 | Cite as

Molecular cloning and characterization of a phenylalanine ammonia-lyase gene (LrPAL) from Lycoris radiata

  • Yumei Jiang
  • Nan Xia
  • Xiaodan Li
  • Wenbiao Shen
  • Lijian Liang
  • Chunyan Wang
  • Ren Wang
  • Feng Peng
  • Bing Xia
Article

Abstract

LrPAL is a novel full-length cDNA isolated from Lycoris radiata by degenerate oligonucleotide primer PCR (DOP-PCR), 3′- and 5′-RACE approaches, harbours an open reading frame (ORF) encoding a 708 amino acid product. Sequence alignment showed that the deduced amino acid sequence of LrPAL shared more than 80% identity with other PAL sequences reported in Arabidopsis thaliana and other plants. RT-PCR revealed that LrPAL transcripts were higher in bud flowers and wilting flowers (5 days after blooming) than in blooming flowers. The transcript levels of LrPAL in leaves were significantly induced by methyl jasmonate (MJ) and nitric oxide (NO), and salicylic acid (SA). Similarly, HPLC analysis showed that galantamine (GAL) content was also higher in bud flowers and wilting flowers than in blooming flowers. The GAL content in leaves was significantly induced by MJ and NO, and inhibited by SA. This study enables us to further elucidate the role of LrPAL in the biosynthesis of GAL in Lycoris radiata at a molecular level.

Keywords

Phenylalanine ammonia-lyase Galantamine biosynthesis Lycoris radiata 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant no. 30700057). We wish to thank Dr. Evan Evans from Tasmanian Institute of Agricultural Research at University of Tasmania for critical reading and linguistic help in the preparation of this manuscript.

Supplementary material

11033_2010_314_MOESM1_ESM.doc (422 kb)
(DOC 423 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yumei Jiang
    • 1
  • Nan Xia
    • 1
    • 2
  • Xiaodan Li
    • 1
  • Wenbiao Shen
    • 3
  • Lijian Liang
    • 1
  • Chunyan Wang
    • 1
  • Ren Wang
    • 1
  • Feng Peng
    • 1
  • Bing Xia
    • 1
  1. 1.Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjingChina
  2. 2.College of PharmacyNanjing University of Chinese MedicineNanjingChina
  3. 3.College of Life SciencesNanjing Agricultural UniversityNanjingChina

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