Molecular Biology Reports

, Volume 38, Issue 3, pp 1869–1874 | Cite as

Over-expression of Zip-13 mRNA in kidney and lung during dietary zinc deficiency in Wistar rats

  • Lu Guo
  • Xiaoyan Hu
  • Tongfu Xu
  • Xin Qi
  • Yang Wan
  • Xiaopeng Liu
  • Yali Jiang
  • Lianying Zhang


Zinc is an essential nutrient for all organisms, which is involved in the function of numerous key enzymes in metabolism. Two gene families have been identified involved in zinc homeostasis. ZnT transporters reduce intracellular zinc while Zip transporters increase intracellular zinc. Previous studies in our laboratory have shown that Zip-1, ZnT-1, Zip-2 and LIV-1 mRNA are associated with zinc level in established human breast cancer in nude mice model. In this study, six zinc transporters: ZnT-1, ZnT-2, ZnT-4, Zip-1, Zip-8 and Zip-13 were chosen. We aim to determine the relation between zinc transporters and zinc level in kidney and lung of Wistar rats. Eighteen Wistar rats were randomly divided into three groups: normal group, zinc-deficiency group and pair-fed group. After 22 days, the rats were killed and organs samples were taken, then zinc transporters mRNA were detected by RT-PCR. Compared with the normal group, Zip-13 shows an up-regulation (P < 0.05) in zinc-deficiency group both in kidney and lung, and Zip-8 was significantly lower (P < 0.05) in zinc-deficiency group in kidney.


Zinc deficiency Zinc transporter Rats Kidney Lung 



Zrt/Irt-like proteins


Zinc transporter


Ethylene diamine tetraacetic acid


  1. 1.
    Vallee BL, Falchuk KH (1993) The biochemical basis of zinc physiology. Physiol Rev 73:79–118PubMedGoogle Scholar
  2. 2.
    Berg JM, Shi Y (1996) The galvanization of biology: a growing appreciation for the roles of zinc. Science 271:1081–1085CrossRefPubMedGoogle Scholar
  3. 3.
    Vallee BL, Auld DS (1990) Zinc coordination, function and structure of zinc enzymes and other proteins. Biochemistry 29:5647–5659CrossRefPubMedGoogle Scholar
  4. 4.
    Fraker PJ, King LE, Laakko T, Vollmer TL (2000) The dynamic link between the integrity of the immune system and zinc status. J Nutr 130(suppl):1399S–1406SPubMedGoogle Scholar
  5. 5.
    Harris ED (2002) Cellular transporters for zinc. Nutr Rev 60:121–124CrossRefPubMedGoogle Scholar
  6. 6.
    Apgar J (1985) Zinc and reproduction. Annu Rev Nutr 5:43–68CrossRefPubMedGoogle Scholar
  7. 7.
    Palmiter RD, Huang L (2004) Efflux and compartmentalization of zinc by members of the SLC30 family of solute carriers. Pflügers Arch 447:744–751CrossRefPubMedGoogle Scholar
  8. 8.
    Eide DJ (2004) The SLC39 family of metal ion transporters. Pflügers Arch 447:796–800CrossRefPubMedGoogle Scholar
  9. 9.
    Reeves PG, Nielsen FH, Fahey GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951PubMedGoogle Scholar
  10. 10.
    Beyersmann D, Haase H (2001) Functions of zinc in signaling, proliferation and differentiation of mammalian cells. Biometals 14:331–341CrossRefPubMedGoogle Scholar
  11. 11.
    Koh JY, Suh SW, Gwag BJ et al (1996) The role of zinc in selective neuronal death after transient global cerebral ischemia. Science 272:1013–1016CrossRefPubMedGoogle Scholar
  12. 12.
    Prasad AS (1996) Zinc and growth. J Am Coll Nutr 15(4):340–344Google Scholar
  13. 13.
    Atkinson SA, Whelan D, Whyte RK, Lönnerdal B (1989) Abnormal zinc content in human milk. Risk for development of nutritional zinc deficiency in infants. Am J Dis Child 143:608–611PubMedGoogle Scholar
  14. 14.
    Chowanadisai W, Lönnerdal B, Kelleher SL (2006) Identification of a mutation in SLC30A2 (ZnT-2) in women with low milk zinc concentration that results in transient neonatal zinc deficiency. J Biol Chem 281:39699–39707CrossRefPubMedGoogle Scholar
  15. 15.
    Kambea T, Yamaguchi-Iwai Y, Sasakib R et al (2004) Overview of mammalian zinc transporters. Cell Mol Life Sci 61:49–68CrossRefGoogle Scholar
  16. 16.
    Sun D, Zhang L (2007) Regulation of zinc transporters by dietary zinc supplement in breast cancer. Mol Biol Rep 34(4):241–247CrossRefPubMedGoogle Scholar
  17. 17.
    Zhang LY, Wang XL (2008) Regulation of zinc transporters by dietary flaxseed lignan in human breast cancer xenografts. Mol Biol Rep 35(4):595–600CrossRefPubMedGoogle Scholar
  18. 18.
    Wang B, Schneider SN, Dragin N, Girijashanker K, Dalton TP, He L, Miller ML, Stringer KF, Soleimani M, Richardson DD, Nebert DW (2007) Enhanced cadmium-induced testicular necrosis and renal proximal tubule damage caused by gene-dose increase in a Slc39a8-transgenic mouse line. Am J Physiol Cell Physiol 292:C1523–C1535CrossRefPubMedGoogle Scholar
  19. 19.
    Himeno S et al (2009) The role of zinc transporters in cadmium and manganese transport in mammalian cells. Biochimie 91(10):1218–1222CrossRefPubMedGoogle Scholar
  20. 20.
    Giulia R, Giuditta P, Ai TT et al (2002) Intracellular distribution of labile Zn(II) and zinc transporter expression in kidney and MDCK cells. Am J Physiol Renal Physiol 283:F1365–F1375Google Scholar
  21. 21.
    Michaelsson G, Ljunghall K, Danielson BG (1980) Zinc in epidermis and dermis in healthy subjects. Acta Derm Venereol 60:295–299PubMedGoogle Scholar
  22. 22.
    Fukada T, Civic N, Furuichi T et al (2008) The zinc transporter SLC39A13/ZIP-13 is required for connective tissue development; its involvement in BMP/TGF-β signaling pathways. PLoS One 3(11):e3642CrossRefPubMedGoogle Scholar
  23. 23.
    Zavadil J, Bottinger EP (2005) TGF-beta and epithelial-to-mesenchymal transitions. Oncogene 24:5764–5774CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Lu Guo
    • 1
  • Xiaoyan Hu
    • 1
  • Tongfu Xu
    • 1
  • Xin Qi
    • 2
  • Yang Wan
    • 2
  • Xiaopeng Liu
    • 2
  • Yali Jiang
    • 1
  • Lianying Zhang
    • 1
  1. 1.Institute of Biochemistry and Molecular BiologyShandong University School of MedicineJinanPeople’s Republic of China
  2. 2.Shandong University School of MedicineJinanPeople’s Republic of China

Personalised recommendations