Molecular Biology Reports

, Volume 38, Issue 3, pp 1813–1819 | Cite as

Molecular analysis of a homogentisate phytyltransferase gene from Lactuca sativa L.

  • Weiwei Ren
  • Lingxia Zhao
  • Lida Zhang
  • Yuliang Wang
  • Lijie Cui
  • Yueli Tang
  • Xiaofen Sun
  • Kexuan Tang


Tocochromanols, usually known as vitamin E, play a crucial role in human and animal nutrition. The enzyme homogentisate phytyltransferase (HPT) performs the first committed step of the vitamin E biosynthetic pathway. The full-length cDNA encoding HPT was isolated from Lactuca sativa L. by rapid amplification of cDNA ends (RACE). The cDNA, designated as LsHPT, was 1,670 bp long containing an open reading frame (ORF) of 1,185 bp which encoded a protein of 395 amino acids. Sequence analysis indicated that the deduced protein, named as LsHPT, shared high identity with other dicotyledonous HPTs. Real-time fluorescent quantitative PCR (qPCR) analysis revealed that LsHPT was preferentially expressed in mature leaves compared with other tissues. When lettuce plants were subjected to drought and high-light stress treatments, LsHPT expression was markedly increased. Expression of LsHPT in Arabidopsis showed that LsHPT could enhance the α-tocopherol biosynthesis in Arabidopsis. Transient expression of LsHPT via agroinfiltration resulted in 9-fold increase in LsHPT mRNA level and nearly 18-fold enhancement in α-tocopherol content compared with the negative controls.


Homogentisate phytyltransferase Lactuca sativa Real-time fluorescent quantitative PCR The vitamin E biosynthetic pathway 



Homogentisate phytyltransferase


Rapid amplification of cDNA ends


Open reading frame


Fresh weight


Photon flux density


High performance liquid chromatography


5-Bromo-4-chloro-3-indolyl-β-d-glucuronic acid





This work was funded by China “973” Program (grant number 2007CB108805) and Shanghai Leading Academic Discipline Project (project number B209).

Supplementary material

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Supplementary material 1 (TIFF 1986 kb)
11033_2010_297_MOESM2_ESM.tif (1.7 mb)
Supplementary material 2 (TIFF 1786 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Weiwei Ren
    • 1
  • Lingxia Zhao
    • 1
  • Lida Zhang
    • 1
  • Yuliang Wang
    • 1
  • Lijie Cui
    • 1
  • Yueli Tang
    • 1
  • Xiaofen Sun
    • 2
  • Kexuan Tang
    • 1
  1. 1.Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D CenterShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  2. 2.State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life SciencesFudan UniversityShanghaiPeople’s Republic of China

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