Molecular Biology Reports

, Volume 38, Issue 3, pp 1807–1812 | Cite as

Effect of polymorphism in the peroxisome proliferator-activated receptor gamma gene on litter size of pigs

  • Guiying Wang
  • Lujun Kong
  • Peng Hu
  • Jinlian Fu
  • Aiguo Wang


The association of polymorphisms in peroxisome proliferator-activated receptor γ (PPARγ) gene with litter size was studied in Large White and Landrace pig. Three SNP loci (P1, P2 and P7) on PPARγ2 gene were determined by PCR–SSCP and the results showed that there were A → G mutations at 220 and 324 bp in 5′-regulator region and at 147 bp in exon 6, respectively. Allele frequencies were analysed in two breeds. Information on 2341 litter records from 564 sows was used to analyse the trait total number born (TNB) and number born alive (NBA). In Large White, TNB and NBA of genotype BB for P2 locus were the lowest, and the TNB and NBA of third and following parities and all parities were 0.74 and 0.51 piglets per litter less (P < 0.001) than those of the highest genotype AB, respectively, but for P1 and P7 locus the beneficial genotype AA were more 0.4–0.8 piglets per litter (P < 0.05) than the inferior genotype AB. In landrace, TNB and NBA of the first parity of genotype BB for P1 locus were 2.0 piglets per litter higher than AA (P < 0.05), but for all parities the TNB and NBA of genotype BB were 0.66 and 0.97 piglets per litter (P < 0.05) higher than AA, respectively. At P2 locus, the TNB and NBA of the second parity of genotype AA were obviously higher than those of AB (P < 0.05). And at P7 locus, the TNB and NBA of each parity of genotype AA were both about 2 piglets per litter more than those of BB (P < 0.05). The results indicated that PPARγ gene was significantly associated with litter size in pigs.


Peroxisome proliferator-activated receptor γ Polymorphism Litter size Pig 



The authors greatly appreciate Beijing Huadu Swine Breeding Company Limited and Beijing Breeding Swine Center for providing samples. This study was funded by National Natural Science Foundation of China (No. 30771540) and National High-Technology Research Development Program of China (No. 2007AA10Z166).

Supplementary material

11033_2010_296_MOESM1_ESM.doc (149 kb)
Supplementary material 1 (DOC 149 kb)


  1. 1.
    Johnson RK, Nielsen MK, Casey DS (1999) Responses in ovulation rate, embryonal survival, and litter traits in swine to 14 generations of selection to increase litter size. J Anim Sci 77:541–557PubMedGoogle Scholar
  2. 2.
    Rothschild MF, Facobson C, Vaske DA, Tuggle C, Wang L, Short T, Eckardt G, Sasaki S, Vincent A, McLaren DG, Southwood O, Van der Steen H, Vileham A, Plastow G (1996) The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proc Natl Acad Sci USA 93:201–205. doi: 10.1073/pnas.93.1.201 CrossRefPubMedGoogle Scholar
  3. 3.
    Gupta A, Dekaney CM, Bazer FW, Madrigal MM, Jaeger LA (1998) Beta transforming growth factors (TGFβ) at the porcine conceptus-maternal interface. Part II: uterine TGFβ bioactivity and expression of immunoreactive TGFβs (TGFβ1, TGFβ2, and TGFβ3) and their receptors (type I and Type II). Biol Reprod 59:911–917. doi: 10.1095/biolreprod59.4.911 CrossRefPubMedGoogle Scholar
  4. 4.
    Wu YP, Wang AG, Li N, Fu JL, Zhao XB (2009) Association with TGF-β1 gene polymorphisms and reproductive performance of Large White pig. Reprod Dom Anim. doi:  10.1111/j.1439-0531.2009.01480.x
  5. 5.
    Vincent AL, Evans G, Short TH, Southwood O, Plastow G, Tuggle C, Rothschild MF (1998) The prolactin receptor gene is associated with increased litter size in pig. In: Proceedings of 6th World Congr Genet Appl Anim Prod Armidale, vol 27. p 15–18Google Scholar
  6. 6.
    Spotter A, Drogemuller C, Hamann H, Distl O (2005) Evidence of a new leukemia inhibitory factor-associated genetic marker for litter size in a synthetic pig line. J Anim Sci 83:2264–2270PubMedGoogle Scholar
  7. 7.
    Lin HC, Liu GF, Wang AG, Kong LJ, Wang XF, Fu LJ (2009) Effect of polymorphism in the leukemia inhibitory factor gene on litter size in Large White pigs. Mol Biol Rep 36:1833–1838. doi: 10.1007/s11033-008-9387-0 CrossRefPubMedGoogle Scholar
  8. 8.
    Schoonjans K, Staels B, Auwerx J (1996) The peroxisome proliferator activated receptors (PPARs) and their effects on lipid metabolism and adipocyte differentiation. Biochim Biophys Acta 1302:93–109. doi: 10.1016/0005-2760(96)00066-5 PubMedGoogle Scholar
  9. 9.
    Elbrecht A, Chen Y, Cullinan CA, Hayes N, Leibowitz M, Moller DE, Berger J (1996) Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors gamma 1 and gamma 2. Biochem Biophys Res Commun 224:431–437. doi: 10.1006/bbrc.1996.1044 CrossRefPubMedGoogle Scholar
  10. 10.
    Toth B, Hornung D, Scholz C, Djalali S, Friese K, Jeschke U (2007) Peroxisome proliferator-activated receptors: new players in the field of reproduction. Am J Reprod Immunol 58:289–310. doi: 10.1111/j.1600-0897.2007.00514.x CrossRefPubMedGoogle Scholar
  11. 11.
    Tontonoz P, Hu E, Spiegelman BM (1994) Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Cell 79:1147–1156. doi: 10.1016/0092-8674(94)90006-X CrossRefPubMedGoogle Scholar
  12. 12.
    Barak Y, Nelson MC, Ong ES, Jones YZ, Ruiz-Lozano P, Chien KR, Koder A, Evans RM (1999) PPARγ is required for placental, cardiac, and adipose tissue development. Mol Cell 4:585–595. doi: 10.1016/S1097-2765(00)80209-9 CrossRefPubMedGoogle Scholar
  13. 13.
    Lappas M, Permezel M, Georgiou HM, Rice GE (2002) Regulation of proinflammatory cytokines in human gestational tissues by peroxisome proliferator-activated receptor-gamma: effect of 15-deoxy-Delta(12, 14)-PGJ(2) and troglitazone. J Clin Endocrinol Metab 87:4667–4672. doi: 10.1210/jc.2002-020613 CrossRefPubMedGoogle Scholar
  14. 14.
    Asami-Miyagishi R, Iseki S, Usui M, Uchida K, Kubo H, Morita I (2004) Expression and function of PPARγ in rat placental development. Biochem Biophys Res Commun 315:497–501. doi: 10.1016/j.bbrc.2004.01.074 CrossRefPubMedGoogle Scholar
  15. 15.
    Kong LJ, Wang AG, Fu JL, Zhang YL, Wang XF (2006) Study on the characteristics of tissue expression of peroxisome proliferators- activated receptor s in pigs. Chin J Anim Sci 42(19):1–4Google Scholar
  16. 16.
    Schoppee PD, Garmey JC, Veldhuis JD (2002) Putative activation of the peroxisome proliferator-activated receptor γ impairs androgen and enhances progesterone biosynthesis in primary cultures of porcine theca cells. Biol Reprod 66:190–198. doi: 10.1095/biolreprod66.1.190 CrossRefPubMedGoogle Scholar
  17. 17.
    Kong LJ, Wang AG, Fu JL, Lai CH, Wang XF, Lin HC (2007) Peroxisome proliferator-activated receptor γ is involved in weaning to estrus of primiparous sows by regulating the expression of hormone genes in hypothalamus-pituitary-ovary axis. Asian Austral J Anim 20(3):340–350Google Scholar
  18. 18.
    Tarrade A, Schoonjans K, Guibourdenche J, Bidart JM, Vidaud M, Auwerx J, Rochette-Egly C, Evain-Brion D (2001) PPARγ/RXRα heterodimers are involved in human CGβ synthesis and human trophoblast differentiation. Endocrinology 142:4504–4514. doi: 10.1210/en.142.10.4504 CrossRefPubMedGoogle Scholar
  19. 19.
    Lehrke M, Lazar MA (2005) The many faces of PPARγ. Cell 123:993–999. doi: 10.1016/j.cell.2005.11.026 CrossRefPubMedGoogle Scholar
  20. 20.
    Keller H, Givel F, Perroud M, Wahli W (1995) Signaling cross-talk between peroxisome proliferator-activated receptor/retinoid X receptor and estrogen receptor through estrogen response elements. Mol Endocrinol 9(7):794–804CrossRefPubMedGoogle Scholar
  21. 21.
    Susan BN, Jeffrey AM, Olivier B, Lawrence K, Walter W, Keiko O, James HS (1997) Retinoid X receptor and peroxisome proliferator-activated receptor activate an estrogen responsive gene independent of the estrogen receptor. Mol Cel Endocrinol 127:27–40. doi: 10.1016/S0303-7207(96)03980-9 CrossRefGoogle Scholar
  22. 22.
    Ulrich K, Shu W, Dennis B, Stephan G, Kristof G, Willa AH, Ronald EL (2002) TGF-β1 induces peroxisome proliferators-activated receptor γ1 and γ2 expression in human THP-1 monocytes. Biochem Biophy Res Comm 297:794–799. doi: 10.1016/S0006-291X(02)02264-7 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyChina Agricultural UniversityBeijingPeople’s Republic of China

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