Advertisement

Molecular Biology Reports

, Volume 38, Issue 3, pp 1793–1798 | Cite as

Polymorphisms in 5′-flanking regions of genes encoding adiponectin, leptin, and resistin are not associated with obesity of Polish children and adolescents

  • Jakub Cieslak
  • Anna Skorczyk
  • Monika Stachowiak
  • Maciej Szydlowski
  • Maria Grzes
  • Paulina Paczynska
  • Bogda Skowronska
  • Katarzyna Majewska
  • Witold Stankiewicz
  • Piotr Fichna
  • Marek Switonski
Article

Abstract

Genes encoding adipokines are important functional candidates for development of obesity. In this study we screened for polymorphism 5′-flanking regions of the adiponectin (ADIPOQ), leptin (LEP) and resistin (RETN) genes in a cohort of Polish obese children and adolescents (n = 243) and a control group of non-obese adults (n = 100). Altogether 13 SNPs (single nucleotide polymorphisms) and 1 InDel (insertion/deletion polymorphism) were found. Among them five polymorphisms, localized in the LEP gene, turned out to be novel, but their distribution was insufficient for association studies. We found no consistent evidence for association between obesity and the SNPs demonstrating minor allele frequency (MAF) above 0.2 (ADIPOQ: −11377C>G, LEP: −2548C>T, 19A>G, RETN: −1300G>A, −1258C>T, −420C>G). Comparison of polymorphisms distribution in patients and control group suggested association with ADIPOQ −11377C>G (Pearson test P = 2.76 × 10−11), however, we did not observe any effect of this polymorphism on BMI or relative BMI (RBMI) within obese patients (P = 0.41). We conclude that the tested SNPs are not useful markers of childhood and adolescence obesity in Polish population.

Keywords

Adiponectin Leptin Resistin Polymorphism Obesity 

Notes

Acknowledgments

Study was founded by the Polish Ministry of Science and Higher Education, grant number: N407 057 32/2522.

References

  1. 1.
    Azuma K, Katsukawa F, Oguchi S, Murata M, Yamazaki H, Shimada A, Saruta T (2003) Correlation between serum resistin level and adiposity in obese individuals. Obes Res 11(8):997–1001CrossRefPubMedGoogle Scholar
  2. 2.
    Bouatia-Naji N, Meyre D, Lobbens S, Séron K, Fumeron F, Balkau B, Heude B, Jouret B, Scherer PE, Dina C, Weill J, Froguel P (2006) ACDC/adiponectin polymorphisms are associated with severe childhood and adult obesity. Diabetes 55(2):545–550CrossRefPubMedGoogle Scholar
  3. 3.
    Chung SS, Choi HH, Kim KW, Cho YM, Lee HK, Park KS (2005) Regulation of human resistin gene expression in cell systems: an important role of stimulatory protein 1 interaction with a common promoter polymorphic site. Diabetologia 48(6):1150–1158CrossRefPubMedGoogle Scholar
  4. 4.
    Cole TJ, Faith MS, Pietrobelli A, Heo M (2005) What is the best measure of adiposity change in growing children: BMI, BMI %, BMI z-score or BMI centile? Eur J Clin Nutr 59(3):419–425CrossRefPubMedGoogle Scholar
  5. 5.
    Degawa-Yamauchi M, Bovenkerk JE, Juliar BE, Watson W, Kerr K, Jones R, Zhu Q, Considine RV (2003) Serum resistin (FIZZ3) protein is increased in obese humans. J Clin Endocrinol Metab 88(11):5452–5455CrossRefPubMedGoogle Scholar
  6. 6.
    Engert JC, Vohl MC, Williams SM, Lepage P, Loredo-Osti JC, Faith J, Doré C, Renaud Y, Burtt NP, Villeneuve A, Hirschhorn JN, Altshuler D, Groop LC, Després JP, Gaudet D, Hudson TJ (2002) 5′ Flanking variants of resistin are associated with obesity. Diabetes 51(5):1629–1634CrossRefPubMedGoogle Scholar
  7. 7.
    Eyzaguirre F, Mericq V (2009) Insulin resistance markers in children. Horm Res 71(2):65–74CrossRefPubMedGoogle Scholar
  8. 8.
    Hillebrand JJ, Geary N (2010) Do leptin and insulin signal adiposity? Forum Nutr 63:111–122CrossRefPubMedGoogle Scholar
  9. 9.
    Hinney A, Vogel CI, Hebebrand J (2010) From monogenic to polygenic obesity: recent advances. Eur Child Adolesc Psychiatry 19(3):297–310CrossRefPubMedGoogle Scholar
  10. 10.
    Hinuy HM, Hirata MH, Forti N, Diament J, Sampaio MF, Armaganijan D, Salazar LA, Hirata RD (2008) Leptin G-2548A promoter polymorphism is associated with increased plasma leptin and BMI in Brazilian women. Arq Bras Endocrinol Metab 52(4):611–616CrossRefGoogle Scholar
  11. 11.
    Hoffstedt J, Eriksson P, Mottagui-Tabar S, Arner P (2002) A polymorphism in the leptin promoter region (−2548 G/A) influences gene expression and adipose tissue secretion of leptin. Horm Metab Res 34(7):355–359CrossRefPubMedGoogle Scholar
  12. 12.
    Jia JJ, Tian YB, Cao ZH, Tao LL, Zhang X, Gao SZ, Ge CR, Lin QY, Jois M (2010) The polymorphisms of UCP1 genes associated with fat metabolism, obesity and diabetes. Mol Biol Rep 37(3):1513–1522CrossRefPubMedGoogle Scholar
  13. 13.
    Kadowaki T, Yamauchi T (2005) Adiponectin and adiponectin receptors. Endocr Rev 26(3):439–451CrossRefPubMedGoogle Scholar
  14. 14.
    Laumen H, Saningong AD, Heid IM, Hess J, Herder C, Claussnitzer M, Baumert J, Lamina C, Rathmann W, Sedlmeier EM, Klopp N, Thorand B, Wichmann HE, Illig T, Hauner H (2009) Functional characterization of promoter variants of the adiponectin gene complemented by epidemiological data. Diabetes 58(4):984–991CrossRefPubMedGoogle Scholar
  15. 15.
    Lebensztejn DM, Wojtkowska M, Skiba E, Werpachowska I, Tobolczyk J, Kaczmarski M (2009) Serum concentration of adiponectin, leptin and resistin in obese children with non-alcoholic fatty liver disease. Adv Med Sci 54(2):177–182CrossRefPubMedGoogle Scholar
  16. 16.
    Lobstein T, Frelut ML (2003) Prevalence of overweight among children in Europe. Obes Rev 4(4):195–200CrossRefPubMedGoogle Scholar
  17. 17.
    Mattevi VS, Zembrzuski VM, Hutz MH (2004) A resistin gene polymorphism is associated with body mass index in women. Hum Genet 115(3):208–212CrossRefPubMedGoogle Scholar
  18. 18.
    Portolés O, Sorlí JV, Francés F, Coltell O, González JI, Sáiz C, Corella D (2006) Effect of genetic variation in the leptin gene promoter and the leptin receptor gene on obesity risk in a population-based case-control study in Spain. Eur J Epidemiol 21(8):605–612CrossRefPubMedGoogle Scholar
  19. 19.
    Silha JV, Krsek M, Skrha JV, Sucharda P, Nyomba BL, Murphy LJ (2003) Plasma resistin, adiponectin and leptin levels in lean and obese subjects: correlations with insulin resistance. Eur J Endocrinol 149(4):331–335CrossRefPubMedGoogle Scholar
  20. 20.
    Stefan N, Bunt JC, Salbe AD, Funahashi T, Matsuzawa Y, Tataranni PA (2002) Plasma adiponectin concentrations in children: relationships with obesity and insulinemia. J Clin Endocrinol Metab 87(10):4652–4656CrossRefPubMedGoogle Scholar
  21. 21.
    Stokić E, Djan M, Vapa Lj, Djan I, Plećas A, Srdić B (2010) Polymorphism Val103Ile of the melanocortin-4 receptor gene in the Serbian population. Mol Biol Rep 37(1):33–37CrossRefPubMedGoogle Scholar
  22. 22.
    Urbanek M, Du Y, Silander K, Collins FS, Steppan CM, Strauss JF III, Dunaif A, Spielman RS, Legro RS (2003) Variation in resistin gene promoter not associated with polycystic ovary syndrome. Diabetes 52(1):214–217CrossRefPubMedGoogle Scholar
  23. 23.
    Wang TN, Huang MC, Chang WT, Ko AM, Tsai EM, Liu CS, Lee CH, Ko YC (2006) G-2548A polymorphism of the leptin gene is correlated with extreme obesity in Taiwanese aborigines. Obes Silver Spring 14(2):183–187CrossRefGoogle Scholar
  24. 24.
    Warodomwichit D, Shen J, Arnett DK, Tsai MY, Kabagambe EK, Peacock JM, Hixson JE, Straka RJ, Province MA, An P, Lai CQ, Parnell LD, Borecki IB, Ordovas JM (2009) ADIPOQ polymorphisms, monounsaturated fatty acids, and obesity risk: the GOLDN study. Obes Silver Spring 17(3):510–517CrossRefGoogle Scholar
  25. 25.
    World Health Organization (2010) Childhood overweight and obesity. http://www.who.int/dietphysicalactivity/childhood/en/. Accessed 5 Mar 2010
  26. 26.
    Computational Biology and Informatics Laboratory at University of Pennsylvania (2010) Transcription Elements Search System. http://www.cbil.upenn.edu/cgi-bin/tess/tess. Accessed 25 Feb 2010

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jakub Cieslak
    • 1
  • Anna Skorczyk
    • 1
  • Monika Stachowiak
    • 1
  • Maciej Szydlowski
    • 1
  • Maria Grzes
    • 1
  • Paulina Paczynska
    • 1
  • Bogda Skowronska
    • 2
  • Katarzyna Majewska
    • 2
  • Witold Stankiewicz
    • 2
  • Piotr Fichna
    • 2
  • Marek Switonski
    • 1
  1. 1.Department of Genetics and Animal BreedingPoznan University of Life SciencesPoznanPoland
  2. 2.Department of Pediatric Diabetes and ObesityPoznan University of Medical SciencesPoznanPoland

Personalised recommendations