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Molecular Biology Reports

, Volume 38, Issue 2, pp 921–927 | Cite as

Molecular characterization and association analysis of porcine adipose triglyceride lipase (PNPLA2) gene

  • Li He Dai
  • Yuan Zhu Xiong
  • Si Wen Jiang
  • Jun Feng Chen
Article

Abstract

The adipose triglyceride lipase (PNPLA2, also known as ATGL) is a novel triacylglycerol (TG) lipase which specifically removes the first fatty acid from the triglyceride molecule generating free fatty acid and diglyceride (DG) in mammalian cells. Here we describe the molecular characterization of the porcine ATGL gene. The full-length cDNA sequence contains a 1,461 bp open reading frame encoding a protein of 486 amino acids with a calculated molecular mass of 53.2 kDa and an isoelectric point of 7.90. The porcine ATGL protein shares high identity with other mammalian ATGL. The ATGL gene contains 9 coding exons, spans approximately 6 kb. The porcine ATGL mRNA was expressed predominantly in backfat, mildly in muscle, small intestine and heart, and almost absent in liver, spleen, lung, stomach, kidney and ovary. Statistical analysis showed the ATGL gene polymorphism (G/A392) was different between Chinese indigenous and introduced commercial western pig breeds, and was highly associated with almost all the fat deposition and carcass traits, including subcutaneous fat thickness, viscera adipose tissue, lean percentage, loin eye traits and even rib numbers.

Keywords

ATGL Pig Characterization Real-time PCR Genomic walking Association analysis 

Notes

Acknowledgments

This work was supported by the grants from the National Key Foundation Research and Development Program of China Grant (2006CB102102) and Natural Science Foundation of Hubei Province (2006ABC008).

Supplementary material

11033_2010_185_MOESM1_ESM.doc (44 kb)
Supplementary material 1 (DOC 44 kb)
11033_2010_185_MOESM2_ESM.doc (140 kb)
Supplementary material 2 (DOC 139 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Li He Dai
    • 1
    • 2
  • Yuan Zhu Xiong
    • 1
  • Si Wen Jiang
    • 1
  • Jun Feng Chen
    • 3
  1. 1.Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Key Lab of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal SciencesHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.The Institute of Animal Husbandry and Veterinary ScienceZhejiang Academy of Agricultural SciencesHangzhouPeople’s Republic of China
  3. 3.Institute of Animal Husbandry and Veterinary ScienceHenan Academy of Agricultural SciencesZhengzhouPeople’s Republic of China

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