Advertisement

Molecular Biology Reports

, Volume 38, Issue 1, pp 411–416 | Cite as

Polymorphism of bovine Y-STR UMN0929 and its correlation with carcass traits in five Chinese beef cattle populations

  • Y. P. Xin
  • L. S. Zan
  • Y. H. Wang
  • Y. F. Liu
  • W. Q. Tian
  • Y. Y. Fan
Article

Abstract

The correlations between Y chromosome polymorphisms and the carcass traits were studied in five Chinese beef cattle populations by PCR, single strand conformation polymorphism and Y-STR sequence analysis. Nine alleles and their frequencies were identified on Y-STR UMN0929 region in Qinchuan (n = 116), Luxi (n = 112), Jinnan (n = 104) pure breeds, Simmental × Qinchuan crossbred (n = 80) and Angus × Qinchuan crossbred (n = 96). The most popular A-176 and B-178 alleles were presented in all 5 cattle populations in the range of 12% (Jinnan) to 66% (Simmental × Qinchuan). The allele I-194 presented Luxi and Angus × Qinchuan. In Qinchun cattle, G-190 and E-186 alleles had bigger effect on BPI (4.23 ± 0.32 and 4.22 ± 0.48 kg/cm, P < 0.01) and CW (325.40 ± 49.42 and 316.73 ± 45.29 kg, P < 0.01), respectively. In Luxi cattle, I-194 allele affected higher BPI (4.08 ± 0.35 kg/cm, P < 0.01) and CW (302.07 ± 17.55 kg, P < 0.01), respectively. In Jinnan cattle breed, H-192 had higher BPI (4.32 ± 0.50 kg/cm, P < 0.05) and CW (327.87 ± 59.37 kg, P < 0.05), respectively. In Simmental × Qinchuan cross breed, C-180 allele affected largely on BPI (5.16 ± 0.25 kg/cm, P < 0.05) and CW (393.16 ± 25.92 kg, P < 0.05). In Angus × Qinchuan cross breed, I-194 had higher BPI (4.43 ± 0.33 kg, P < 0.05) and CW (346.63 ± 29.77 kg, P < 0.05). Correlations between alleles and other carcass traits (net meat weight, top grade weight, slaughter rate, net meat rate, loin-eye muscle area, carcass length, meet tenderness and shear force) were also analyzed using mixed-effect model. Cattle Y-STR UMN0929 loci alleles and its correlation with carcass traits in beef cattle populations could be implemented into the cattle breeding program for choosing beef cattle with better carcass traits.

Keywords

Beef cattle Y chromosome Gene polymorphism Economic traits Y-STR UMN0929 

Abbreviations

STR

Short tandem repeat

GD

Gene diversity

PIC

The polymorphic information content

Notes

Acknowledgments

The authors are grateful to Dr. Bin Wu for the critical reading and editing of the manuscript. This study was financially supported by the National “863” Program of China (No. 2006AA10Z1A1, 2008AA101010), MATS-Beef Cattle System.

References

  1. 1.
    Jobling MA, Tyler-Smith C (2003) The human Y chromosome: an evolutionary marker comes of age. Nat Rev Genet 4:598–612CrossRefPubMedGoogle Scholar
  2. 2.
    Gill P, Brenner C, Brinkmann B, Budowle B, Carracedo A, Jobing MA, de Knijff P, Kayser M, Krawczak M, Mayr R, Morling B, Olaisen B, Pascali V, Prina M, Roewer L, Schneider PM, Sajantila A, Tyler-Smith C (2001) DNA Commission of the International Society of Forensic Genetics: recommendations on forensic analysis using Y-chromosome STRs. Forensic Sci Int 124:5–10CrossRefPubMedGoogle Scholar
  3. 3.
    Liu WS, Mariani P, Beattie CW, Alexander LJ, Ponce De Leon FA (2002) A radiation hybrid map for the bovine Y chromosome. Mamm Genome 13:320–326CrossRefPubMedGoogle Scholar
  4. 4.
    Edwards CJ, Gaillard C, Bradley DG, MacHugh DE (2000) Y-specific microsatellite polymorphisms in a range of bovid species. Anim Genet 31(2):127–130CrossRefPubMedGoogle Scholar
  5. 5.
    Giovambattista G, Ripoli MV, De Luca JC, Mirol PM, Liron JP, Dulout FN (2000) Male mediated introgression of Bos indicus genes into Argentine and Bolivian Creole cattle breeds. Anim Genet 31(5):302–305CrossRefPubMedGoogle Scholar
  6. 6.
    Hanotte O, Tawah CL, Bradley DG, Ochieng JW, Verjee Y, Hill EW, Rege JE (2000) Geographic distribution and frequency of a taurine Bos taurus and an indicine Bos indicus Y specific allele amongst sub-saharan African cattle breeds. Mol Ecol 9(4):387–396CrossRefPubMedGoogle Scholar
  7. 7.
    Liu YF, Zan LS, Li K, Zhao SP, Xin YP, Lin Q, Tian WQ, Wang ZW (2010) A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Mol Biol Rep 37:429–434CrossRefPubMedGoogle Scholar
  8. 8.
    Kima NK, Chob S, Leea SH, Parke HR, Leee CS, Choa YM, Choyd YH, Yoonc D, Ima SK, Parka EW (2008) Proteins in longissimus muscle of Korean native cattle and their relationship to meat quality. Meat Sci 80:1068–1073CrossRefGoogle Scholar
  9. 9.
    Walsh PS, Metzger DA, Higuchi R (1991) Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506–513PubMedGoogle Scholar
  10. 10.
    Liu WS, Beattie CW, Ponce de León FA (2003) Bovine Y chromosome microsatellite polymorphisms. Cytogenet Genome Res 102(1–4):53–58CrossRefPubMedGoogle Scholar
  11. 11.
    Applied Biosystems, Amp Fistr (2001) Identifier PCR amplification user’s manual. Applied Biosystems, Foster CityGoogle Scholar
  12. 12.
    Stephens M, Smith NJ, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. Am J Hum Genet 68:978–989CrossRefPubMedGoogle Scholar
  13. 13.
    Hellborg L, Ellegren H (2003) Y chromosome conserved anchored tagged sequences (YCATS) for the analysis of mammalian male-specific DNA. Mol Ecol 12:283–291CrossRefPubMedGoogle Scholar
  14. 14.
    Liu HY, Tian WQ, Zan LS, Wang HB, Cui H (2010) Mutations of MC4R gene and its association with economic traits in Qinchuan cattle. Mol Biol Rep 37:535–540CrossRefPubMedGoogle Scholar
  15. 15.
    Ginja C, Gama LTD, Cecilia M, Penedo T (2009) Y Chromosome haplotype analysis in Portuguese cattle breeds using SNPs and STRs. J Hered 100(2):148–157CrossRefPubMedGoogle Scholar
  16. 16.
    Li F, Chen H, Lei CZ, Ren G, Wang J, Li ZJ, Wang JQ (2010) Novel SNPs of the bovine GAD1/gad67 gene and their association with growth traits in three native Chinese cattle breeds. Mol Biol Rep 37:501–505CrossRefPubMedGoogle Scholar
  17. 17.
    Xin YP, Zan LS, Liu YF, Liu HY, Tian WQ, Fan YY, Huang L (2009) Population genetic analysis of 6 Y-STR loci in Chinese northwestern Qinchuan yellow cattle breed. Mol Biol Rep. doi 10.1007/s11033-009-9875-x

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Y. P. Xin
    • 1
  • L. S. Zan
    • 1
  • Y. H. Wang
    • 2
  • Y. F. Liu
    • 1
  • W. Q. Tian
    • 3
  • Y. Y. Fan
    • 1
  1. 1.The Key Laboratory of National Beef Cattle Improvement Center, College of Animal Science and TechnologyNorthwest A&F UniversityYanglingChina
  2. 2.CSIRO Livestock IndustriesSt LuciaAustralia
  3. 3.Yangling Vocational Technical CollegeYanglingChina

Personalised recommendations