AtMYB12 gene: a novel visible marker for wheat transformation
Efficiency of plant transformation is less than optimal for many important species, especially for monocots which are traditionally recalcitrant to transformation, such as wheat. And due to limited number of selectable marker genes, identification or selection of those cells that have integrated DNA into appropriate plant genome and to regenerate fully developed plants from the transformed cells, becomes even more difficult. Some of the widely used marker genes belong to the categories of herbicide or antibiotic resistance genes and flourescent protein genes. As they become an integral part of plant genome along with promoters prokaryotic or eukaryotic origin, there are certain health and environmental concerns about the use of these reporter genes. These marker genes are also inefficient with respect to time and space. In this study we have found a novel visible selection agent AtMYB12, to screen transgenic wheat, with in days after transformation. Transformed coleoptiles as well as cells regenerating from transformed cultured scutella, phenotypically exhibit purple pigmentation, making selection possible in limited and reasonable cost, time and space.
KeywordsAtMYB12 Marker Wheat
This work was support by the national Natural Science Foundation of China (30871524), the National S&T Major Project of the People's Republic of China (2008ZX08002004), (2008ZX08010-004), and National Science Foundation of China (2009DFB30340), (2009DFB20290).
- 2.Arago FJL, Brasileiro ACM (2002) Positive, negative and marker-free strategies for transgenic plant selection. Braz J Plant Physiol 14:1–10Google Scholar
- 14.Haseloff J, Siemering KR (2005) The uses of green fluorescent protein in plants. Green fluorescent protein: properties, applications and protocols. Methods Biochem Anal 47:259Google Scholar
- 15.James C (2004) Global status of commercialized biotech/GM crops: 2004. ISAAA Briefs 32:1–12Google Scholar
- 26.Shewry PR, Tatham AS, Fido RJ (1995) Separation of plant proteins by electrophoresis. Methods in molecular biology—plant gene transfer and expression protocols, vol 49. pp 399–422Google Scholar
- 28.Stacey J, Isaac P (1994) Isolation of DNA from plants. Methods in molecular biology—protocols for nucleic acid analysis by nonradioactive probes, vol 28. pp 9–15Google Scholar