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Recombinant expression of human nerve growth factor beta in rabbit bone marrow mesenchymal stem cells

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Abstract

Nerve growth factor (NGF) is required for the differentiation and maintenance of sympathetic and sensory neurons. In the present study, the recombinant expression of human nerve growth factor beta (hNGF-β) gene in rabbit bone marrow mesenchymal stem cells (rMSCs) was undertaken. Recombinant vector containing hNGF-β was constructed and transferred into rMSCs, the expressions of the exogenous in rMSCs were determined by reverse transcriptase PCR (RT-PCR), ELISA and Western blot, whereas the biological activity of recombinant hNGF-β was confirmed using PC12 cells and cultures of dorsal root ganglion neurons from chicken embryos. The results showed that the hNGF-β gene expressed successfully in the rMSCs, a polypeptide with a molecular weight of 13.2 kDa was detected. The maximal expression level of recombinant hNGF-β in rMSCs reached 126.8012 pg/106 cells, the mean concentration was 96.4473 pg/106 cells. The recombinant hNGF-β in the rMSCs showed full biological activity when compared to commercial recombinant hNGF-β.

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Abbreviations

NGF:

Nerve growth factor

RT-PCR:

Reverse transcriptase-polymerase chain reaction

MSCs:

Bone marrow mesenchymal stem cells

RMSCs:

Rabbit mesenchymal stem cells

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  1. Department of Internal Neurology, Second Affiliated Hospital, Zhengzhou University, No. 2 JingBa Road, Zhengzhou, Henan, 450014, People’s Republic of China

    Bo-Sheng Fan & Ji-Yu Lou

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  1. Bo-Sheng Fan
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  2. Ji-Yu Lou
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Correspondence to Ji-Yu Lou.

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Fan, BS., Lou, JY. Recombinant expression of human nerve growth factor beta in rabbit bone marrow mesenchymal stem cells. Mol Biol Rep 37, 4083–4090 (2010). https://doi.org/10.1007/s11033-010-0068-4

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  • DOI: https://doi.org/10.1007/s11033-010-0068-4

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