First report on the molecular prevalence of Mycoplasma capricolum subspecies capripneumoniae (Mccp) in goats the cause of contagious caprine pleuropneumonia (CCPP) in Balochistan province of Pakistan
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Contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp) is a disease of goats which causes high morbidity and mortality and is reported in many countries of the world. There are probably no reports on the molecular prevalence of Mccp, Mycoplasma capricolum subsp. capricolum (Mcc) and Mycoplasma putrefaciens (Mp) in Balochistan and any other part of Pakistan. Thirty goats (n = 30) with marked respiratory symptoms were selected and procured from forty goat flocks in Pishin district of Balochistan in 2008. The genomic deoxyribonucleic acid (DNA) from the lung samples (n = 30) of the slaughtered goats was purified and subjected to polymerase chain reaction (PCR) assays for the presence of Mycoplasma mycoides cluster members and Mp. The PCR-RFLP (restriction fragment length polymorphism) was also used to further confirm the Mccp. Of the thirty lung samples 17 (56.67%) were positive for the molecular prevalence of Mcc, Mccp and Mp. In total the molecular prevalence was observed as 17.65% for Mccp (n = 3), 70.59% for Mcc (n = 12) and 11.76% for Mp (n = 2). The RFLP profile has also validated the PCR results of Mccp by yielding two bands of 190 and 126 bp. The results of PCR-RFLP coupled with the presence of fibrinous pleuropneumonia and pleurisy during postmortem of goats (n = 3) strongly indicated the prevalence of CCPP in this part of world. Moreover the prevalence of Mcc and Mp is also alarming in the study area. We report for the very first time the molecular prevalence of Mcc, Mccp, and Mp in the lung tissues of goats in the Pishin district of Balochistan, Pakistan.
KeywordsGoats Mycoplasma CCPP PCR Balochistan Pakistan
The authors are highly thankful to Pakistan Agriculture Research Council (PARC) Islamabad for granting the project on Caprine Mycoplasmosis in Balochistan under the Agriculture Linkage Programme (ALP), the Center for Advanced Studies in Vaccinology & Biotechnology (CASVAB), University of Balochistan in providing all the paraphernalia for the molecular studies, Dr Jamil and Naseeb ullah, Faculty of Biotechnology and Bioinformatics, Balochistan University of Information Technology and Management Sciences (MUITMS) for their technical cooperation and inspiration and above all the excellent guidance and extra ordinary help of the members of Mycoplasma Group at Veterinary Laboratories Agency (Weybridge) UK is highly appreciated.
- 3.OIE (2008) Contagious caprine pleuropneumonia. In: Manual of standards for diagnostic tests and vaccines. Office International des Epizooties, Paris, pp 1000–1012Google Scholar
- 4.Manso-Silván L, Vilei EM, Sachse K, Djordjevic SP, Thiaucourt F, Frey J (2009) Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. Bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri. Int J Syst Evol Microbiol 59:1353–1358CrossRefPubMedGoogle Scholar
- 8.Rubina A, Sarwat NM (2006) Science et changements planétaires. Sécheresse 17(1):203–209Google Scholar
- 9.Anon (2006) Economic Survey, Government of Pakistan. Fin. Div. Eco. Adv. Wing. Islamabad, PakistanGoogle Scholar
- 10.Tariq MA (1980) Studies on the incidence, epizootology and development of effective vaccines for the control of contagious caprine pleuropneumonia, contagious agalactia in sheep and goats in Balochistan (Annual report), Livestock Department Government of Balochistan (Pakistan)Google Scholar
- 11.Awan MA, Siddique M, Abbas F, Babar S, Mahmood I, Samad A (2004) Isolation and identification of Mycoplasmas from pneumonic lungs of goats. J App Em Sc 1(1):45–50Google Scholar
- 12.Shankster S, Ayling RD, Lenfant D, Mercier P, Nicholas RAJ (2002) Development and evaluation of a PCR to detect Mycoplasma putrefaciens directly from goats. Res Vet Sci 72(Suppl A):26 (Abstracts)Google Scholar
- 13.Al-M Waleed, Halablab MA, Abo-Shehada MN, Miles K, McAuliffe L, Nicholas RAJ (2006) Isolation and molecular identification of small ruminant mycoplasmas in Jordan. J Small Rum Res 5:106–112Google Scholar
- 16.Mercier P, Lenfant D, Poumarat F, Perrin G (2001) Prevalence of mycoplasma infection within French milking caprine herds. In: Poveda JB, Fernandéz A, Frey J, Johansson KE (eds) Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics, vol 5. European Commission, Brussels, Belgium, pp 130–133Google Scholar
- 19.Nicholas RAJ (2002) Contagious caprine pleuropneumonia. In: Recent advances in goat diseases. International Veterinary Information Services. Ithaca, NY. www.ivis.org