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Molecular Biology Reports

, Volume 37, Issue 7, pp 3319–3325 | Cite as

Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton

  • Quan Sun
  • Yingfan Cai
  • Yongfang Xie
  • Jianchuan Mo
  • Youlu Yuan
  • Yuzhen Shi
  • Shengwei Li
  • Huaizhong Jiang
  • Zheng Pan
  • Yunling Gao
  • Min Chen
  • Xiaohong He
Article

Abstract

To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation System) indicate that these genes are involved in 68 biochemical pathways. These findings suggest that the related defense response, gossypol biosynthesis pathway and other complex regulation may be associated with pigment gland morphogenesis in cotton. The results may provide a basis for further study and serve as a guide for related research.

Keywords

Cotton (GossypiumGenechip Gene expression profile Pigment gland Morphogenesis 

Notes

Acknowledgments

This work was supported by grants from the National Nature Science Foundation of China (No. 30771311, 30440032) Natural Sciences Foundation of Chongqing of China (No. Chongqing cstc 2007 BB1328 and BB1329), and the Committee of Sciences and Technology of Nan’an in Chongqing (2008), and Natural Sciences Foundation of Chongqing University of Posts and Telecommunications (No. A2008-33).

Supplementary material

11033_2009_9918_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)

References

  1. 1.
    Agrawal AA, Karban R (2000) Specificity of constitutive and induced resistance: pigment glands influence mites and caterpillars on cotton plants. Entomol Exp Appl 96:39–49CrossRefGoogle Scholar
  2. 2.
    Akingbemi BT, Rao PV, Aire TA (1996) Ethanol intake may modify gossypol toxicosis in the rat. J Appl Toxicol 16:375–380CrossRefPubMedGoogle Scholar
  3. 3.
    Alford BB, Liepa GU, Vanbeber AD (1996) Cottonseed protein: what does the future hold? Plant Foods Hum Nutr 49:1–11CrossRefPubMedGoogle Scholar
  4. 4.
    Badawy SZ, Souid AK, Cuenca V, Montalto N, Shue F (2007) Gossypol inhibits proliferation of endometrioma cells in culture. Asian J Androl 9:388–393CrossRefPubMedGoogle Scholar
  5. 5.
    Band V, Hoffer AP, Band H, Rhinehardt AE, Knapp RC, Matlin SA, Anderson DJ (1989) Antiproliferative effect of gossypol and its optical isomers on human reproductive cancer cell lines. Gynecol Oncol 32:273–277CrossRefPubMedGoogle Scholar
  6. 6.
    Benz CC, Keniry MA, Ford JM, Townsend AJ, Cox FW, Palayoor S, Matlin SA, Hait WN, Cowan KH (1990) Biochemical correlates of the antitumor and antimitochondrial properties of gossypol enantiomers. Mol Pharmacol 37:840–847PubMedGoogle Scholar
  7. 7.
    Bertrand JA, Sudduth TQ, Condon A, Jenkins TC, Calhoun MC (2005) Nutrient content of whole cottonseed. J Dairy Sci 88:1470–1477CrossRefPubMedGoogle Scholar
  8. 8.
    Bodenhausen N, Reymond P (2007) Signaling pathways controlling induced resistance to insect herbivores in Arabidopsis. Mol Plant Microbe Interact 20:1406–1420CrossRefPubMedGoogle Scholar
  9. 9.
    Borevitz JO, Xia Y, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12:2383–2393CrossRefPubMedGoogle Scholar
  10. 10.
    Cai YF, Mo JC, Zeng Y, Ren WW, Xu Y, Wang SH, Chen F (2003) Cloning of cDNAs of differentially expressed genes in the development of special pigment gland of cotton by suppression subtractive hybridization. J Beijing Univ For 25:6–10 (Chinese)Google Scholar
  11. 11.
    Cai Y, Zhang H, Zeng Y, Mo J, Bao J, Miao C, Bai J, Yan F, Chen F (2004) An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton. J Biosci 29:67–71CrossRefPubMedGoogle Scholar
  12. 12.
    Chen D, Ye H, Li G (2000) Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation. Plant Sci 155:179–185CrossRefPubMedGoogle Scholar
  13. 13.
    Coutinho EM (2002) Gossypol: a contraceptive for men. Contraception 65:259–263CrossRefPubMedGoogle Scholar
  14. 14.
    Delannoy E, Jalloul A, Assigbetsé K, Marmey P, Geiger JP, Lherminier J, Daniel JF, Martinez C, Nicolel M (2003) Activity of Class III peroxidases in the defense of cotton to bacterial blight. MPMI 16:1030–1038CrossRefPubMedGoogle Scholar
  15. 15.
    Delannoy E, Marmey P, Jalloul A, Etienne H, Nicole M (2006) Molecular analysis of class III peroxidases from cotton. J Cotton Sci 10:53–60Google Scholar
  16. 16.
    Du L, Ge F, Zhu S, Parajulee MN (2004) Effect of cotton cultivar on development and reproduction of Aphis gossypii (Homoptera: Aphididae) and its predator Propylaea japonica (Coleoptera: Coccinellidae). J Econ Entomol 97:1278–1283CrossRefPubMedGoogle Scholar
  17. 17.
    Gerasimidis K, Fillou DT, Babatzimcpoulou M, Tassou K, Katsikas H (2007) Preparation of an edible cottonseed protein concentrate and evaluation of its functional properties. Int J Food Sci Nutr 58:486–490CrossRefPubMedGoogle Scholar
  18. 18.
    Gonzalez-Garza MT, Mata-Cárdenas BD, Said-Fernández S (1989) High susceptibility of five axenic Entamoeba histolytica strains to gossypol. Trans R Soc Trop Med Hyg 83:522–524CrossRefPubMedGoogle Scholar
  19. 19.
    Hayashi M, Takahashi H, Tamura K, Huang J, Yu LH, Kawai-Yamada M (2005) Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice. PNAS 102:7020–7025CrossRefPubMedGoogle Scholar
  20. 20.
    Hedin PA, Parrot WL, Jenkins JN (1992) Relationship of glands, cotton square terpenoid aldehydes and other allelochemicals to larvae growth of Heliothis virescens (Lepidoptera: Noctui). J Econ Entomol 85:359–364Google Scholar
  21. 21.
    Heywood R (1988) The toxicology of gossypol acetic acid and (−)-gossypol. Contraception 37:185–190CrossRefPubMedGoogle Scholar
  22. 22.
    Joseph AEA, Matlin SA, Knox P (1986) Cytotoxicity of enantiomers of gossypol. Br J Cancer 54:511–513PubMedGoogle Scholar
  23. 23.
    Kneusel RE, Matern U, Nlcolay K (1989) Formation of transcaffeoyl-COA from trans-4-coumaroyl-COAb y Znz+-dependent enzymes in cultured plant cells and its activation by an elicitor-induced pH shift. Arch Biochem Biophys 269:455–462CrossRefPubMedGoogle Scholar
  24. 24.
    Kwak SS, Kamiya Y, Sakurai A, Takahishi N, Graebe JE (1988) Partial-purification and characterization of gibberellin 3beta-hydroxylase from immature seeds of Phaseolus vulgaris L. Plant Cell Physiol 29:935–943Google Scholar
  25. 25.
    Liu S, Kulp SK, Sugimoto Y, Jiang J, Chang HL, Dowd MK, Wan P, Lin YC (2002) The (−)-enantiomer of gossypol possesses higher anticancer potency than racemic gossypol in human breast cancer. Anticancer Res 22:33–38PubMedGoogle Scholar
  26. 26.
    Lopez LM, Grimes DA, Schulz KF (2005) Nonhormonal drugs for contraception in men: a systematic review. Obstet Gynecol Surv 60:746–752CrossRefPubMedGoogle Scholar
  27. 27.
    Lusas EW, Jividen GM (1987) Glandless cottonseed: a review of the first 25 years of processing and utilization research. J Am Oil Chem Soc 64:839–854CrossRefGoogle Scholar
  28. 28.
    Mao X, Cai T, Olyarchuk JG, Wei L (2005) Automated genome annotation and pathway identification using the KEGG orthology (KO) as a controlled vocabulary. Bioinformatics 21:3787–3793CrossRefPubMedGoogle Scholar
  29. 29.
    Martin GS, Liu J, Benedict CR, Stipanovic RD, Magil CW (2003) Reduced levels of cadinane sesquiterpenoids in cotton plants expressing antisense (+)-δ-cadinene synthase. Phytochemistry 62:31–38CrossRefPubMedGoogle Scholar
  30. 30.
    Matlin SA, Zhou RH, Bialy G, Blye RP, Naqvi RH, Lindberg MC (1985) (−)-Gossypol: an active male antifertility agent. Contraception 31:141–149CrossRefPubMedGoogle Scholar
  31. 31.
    McMichael SC (1959) Hopi. Cotton, a source of cotton seed free of gossypol pigment. Agron J 51:630CrossRefGoogle Scholar
  32. 32.
    McMichael SC (1960) Combined effects of glandless genes gl2 and gl3 on pigment glands in the cotton plant. Agron J 52:385–386CrossRefGoogle Scholar
  33. 33.
    Miravalle RJ, Hyer AH (1962) Identification of the Gl2 gl2 Gl3 gl3 genotype in breeding for glandless cottonseed. Crop Sci 2:395–397CrossRefGoogle Scholar
  34. 34.
    Naqvi RH, Lindberg MC, Bialy G, Blye RP, Matlin SA, Zhou RH (1987) Comparative antifertility effects of gossypol enantiomers in male hamsters. Int J Androl 10:619–623CrossRefPubMedGoogle Scholar
  35. 35.
    Oliver CL, Miranda MB, Shangary S, Land S, Wang S, Johnson DE (2005) (−)-Gossypol acts directly on the mitochondria to overcome Bcl-2- and Bcl-X(L)-mediated apoptosis resistance. Mol Cancer Ther 4:23–31CrossRefPubMedGoogle Scholar
  36. 36.
    Patil MA, Pierce ML, Phillips AL, Venters BJ, Essenberg M (2005) Identification of genes up-regulated in bacterial-blight-resistant upland cotton in response to inoculation with Xanthomonas campestris pv malvacearum. Physiol Mol Plant Pathol 67:319–335CrossRefGoogle Scholar
  37. 37.
    Sang GW, Zhang Y, Qi S, Shen K, Lu F, Zhao X, Wang M, Liu X, Yuan Y (1980) Chronic toxicity of gossypol and the relationship to its metabolic fate in dogs and monkeys. Acta Pharmacol Sin 1:39–43Google Scholar
  38. 38.
    Shandilya LN, Clarkson TB, Adams MR, Lewis JC (1982) Effects of gossypol on reproductive and endocrine functions of male cynomolgus monkeys. Biol Reprod 27:241–252CrossRefPubMedGoogle Scholar
  39. 39.
    Stipanovic RD, Lopez JD Jr, Dowd MK, Puckhaber LS, Duke SE (2006) Effect of racemic and (+)- and (−)-gossypol on the survival and development of Helicoverpa zea larvae. J Chem Ecol 32:959–968CrossRefPubMedGoogle Scholar
  40. 40.
    Sunilkumar G, Campbell LM, Puckhaber L, Stipanovic RD, Rathore KS (2006) Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. PNAS 103:18054–18059CrossRefPubMedGoogle Scholar
  41. 41.
    Tegos G, Stermitz FR, Lomovskaya O, Lewis K (2002) Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrob Agents Chemother 46:3133–3141CrossRefPubMedGoogle Scholar
  42. 42.
    Townsend BJ, Poole A, Blake CJ, Llewellyn DJ (2005) Antisense suppression of a (+)-delta-cadinene synthase gene in cotton prevents the induction of this defense response gene during bacterial blight infection but not its constitutive expression. Plant Physiol 138:516–528CrossRefPubMedGoogle Scholar
  43. 43.
    Vaissayre M, Hau B (1985) New results on the susceptibility of glandless cotton varieties to phyllophagous insects. Cotton Fibres Trop 40:159–168Google Scholar
  44. 44.
    Wang JY, Cai Y, Gou JY, Mao YB, Xu YH, Jiang WH, Chen XY (2004) VdNEP, an elicitor from Verticillium dahliae, induces cotton plant wilting. Appl Environ Microb 70:4989–4995CrossRefGoogle Scholar
  45. 45.
    Weinbauer GF, Rovan E, Frick J (1983) Toxicity of gossypol at antifertility dosages in male rats. Andrologia 15:213–221CrossRefPubMedGoogle Scholar
  46. 46.
    Wu J, Mao X, Cai T, Luo J, Wei L (2006) KOBAS server: a web-based platform for automated annotation and pathway identification. Nucleic Acids Res 34:W720–W724CrossRefPubMedGoogle Scholar
  47. 47.
    Ye W, Chang HL, Wang LS, Huang YW, Shu S, Dowd MK, Wan PJ, Sugimoto Y, Lin YC (2007) Modulation of multidrug resistance gene expression in human breast cancer cells by (−)-gossypol-enriched cottonseed oil. Anticancer Res 27:107–116PubMedGoogle Scholar
  48. 48.
    Zhang XL, Jin L, Zhang TZ (2001) A new upland cotton cultivar with glanded plant and low gossypol content seed. Sci Agric Sin 34:564–567Google Scholar
  49. 49.
    Zhu SY, Chen JX (2005) Advances in research on genetic analysis of gossypol g land and molecular breeding of low-gossypol cotton. Chin Agric Sci Bull 21:57–60Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Quan Sun
    • 1
  • Yingfan Cai
    • 1
  • Yongfang Xie
    • 1
  • Jianchuan Mo
    • 1
  • Youlu Yuan
    • 2
  • Yuzhen Shi
    • 2
  • Shengwei Li
    • 1
  • Huaizhong Jiang
    • 1
  • Zheng Pan
    • 1
  • Yunling Gao
    • 1
  • Min Chen
    • 1
  • Xiaohong He
    • 1
  1. 1.College of BioinformationChongqing University of Posts and TelecommunicationsChongqingChina
  2. 2.Cotton Research Institute of Chinese Academy of Agricultural SciencesAnyangChina

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