Gene expression profiling during gland morphogenesis of a mutant and a glandless upland cotton
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To identify genes involved in pigment gland morphogenesis in cotton, gene expression was profiled using genechip (Affymetrix) during pigment gland morphogenesis in cotton variety Xiangmian-18, which has glandless seeds but glanded plants, and a glandless line, N5. The results showed that 303 genes were differentially expressed by a factor greater than two during gland morphogenesis; 59% (180) of these genes shared similarity with known genes in GenBank. These genes play roles in defense response, response to oxidative stress, peroxidase activity, and other metabolic pathways. KOBAS (KEGG Orthology-Based Annotation System) indicate that these genes are involved in 68 biochemical pathways. These findings suggest that the related defense response, gossypol biosynthesis pathway and other complex regulation may be associated with pigment gland morphogenesis in cotton. The results may provide a basis for further study and serve as a guide for related research.
KeywordsCotton (Gossypium) Genechip Gene expression profile Pigment gland Morphogenesis
This work was supported by grants from the National Nature Science Foundation of China (No. 30771311, 30440032) Natural Sciences Foundation of Chongqing of China (No. Chongqing cstc 2007 BB1328 and BB1329), and the Committee of Sciences and Technology of Nan’an in Chongqing (2008), and Natural Sciences Foundation of Chongqing University of Posts and Telecommunications (No. A2008-33).
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