Molecular Biology Reports

, Volume 37, Issue 7, pp 3165–3170 | Cite as

PriA participates in nascent DNA synthesis in Escherichia coli

  • Yen-Yu Chen
  • Helen Huang
  • Tzu-Chien V. Wang


The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig + polA + strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA.


Discontinuous DNA replication PriA, B, C Rep Ligase Pol I 



We thank Dr. Kendric C. Smith for his helpful suggestions. This work was supported by Chang Gung Medical Research Grant CMRPD140013, and National Science Council Research Grant NSC95-2311-B182-008-MY3 of Taiwan.


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© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Molecular and Cellular BiologyChang Gung UniversityKwei-San, Tao-YuanTaiwan

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