Molecular Biology Reports

, Volume 37, Issue 7, pp 3081–3087 | Cite as

Prediction and validation of conservative microRNAs of Solanum tuberosum L.

  • Wenzheng Yang
  • Xin Liu
  • Jianguang Zhang
  • Junli Feng
  • Chao Li
  • Jishuang Chen


Potato (Solanum tuberosum) is an important crop around the world, and accounts for a significant amount of the food consumed by humans. However, little information is available about potato miRNAs which play important regulatory roles in plant growth and development. In the present study, computational prediction of potential miRNAs from potato revealed 71 miRNAs belonging to 48 families. Amongst these 71 mRNAs, 65 were predicted for the first time. Most potato miRNA families have one to three members, and sequence analysis showed that the candidate pre-miRNA sequences varied from 48 to 224 bp in length. To verify the predicted miRNAs, specific stem-loop RT primers were designed and real-time PCR assays were used to profile the expression levels of seven miRNAs from different tissues of potato. The results showed that all the selected miRNAs were successfully amplified. Most of them had their highest expression levels in leaves, and the lowest levels in the stem, while miR159 and miR164 presented a different expression pattern. The specific expression levels of each miRNAs in the tested tissues may be related to their particular functions in regulating potato vegetative growth and organ development.


Solanum tuberosum miRNA Prediction Stem-loop real-time RT–PCR 



Shoot apical meristem


Expressed sequence tags


Minimal free energies


Minimal free energy index


RNA-induced silencing complex




Gibberellins Myeloblastosis



This work was supported by the National High-Tech Development Project (863: 2008AA 10Z129).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Wenzheng Yang
    • 1
  • Xin Liu
    • 1
  • Jianguang Zhang
    • 1
  • Junli Feng
    • 1
  • Chao Li
    • 1
  • Jishuang Chen
    • 1
  1. 1.Institute of BioengineeringZhejiang Sci-Tech UniversityHangzhouPeople’s Republic of China

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