Molecular identification of oriental medicinal plant Anemarrhena asphodeloides Bunge (‘Jimo’) by multiplex PCR
- 212 Downloads
Anemarrhena asphodeloides Bunge (Jimo) is one of the most popular and valuable plant species in many countries, including China, Korea, and Japan. The current commercial products such as Sagan, Chog-chag-yag, and Shig-ban-pung which are most similar to Jimo roots, were used for more reliable authentication method. Polymerase chain reaction (PCR) analysis of the trnL-F region has been proved to be an appropriate method for the identification of species in the A. asphodeloides genus. A single nucleotide polymorphism (SNP) has been identified in Jimo, Sagan, Chog-chag-yag, and Shig-ban-pung. The specific PCR primers were designed from the SNP to differentiate the A. asphodeloides (Jimo) from Sagan, Chog-chag-yag, and Shig-ban-pung via multiplex PCR. The established multiplex-PCR method for the rapid detection of the Jimo in a single reaction was determined to be effective for the differentiation of Jimo (A. asphodeloides). We therefore present an effective method for the genetic identification of the A. asphodeloides.
KeywordsAnemarrhena asphodeloides trnL-F Single nucleotide polymorphism Multiplex PCR
This research was supported by a grant (07092KFDA335) from Korea Food & Drug Administration in 2007.
- 4.Zhou T, Zhu Z, Wang C, Fan G, Peng J, Chai Y, Wu Y (2007) On-line purity monitoring in high-speed counter-current chromatography: application of HSCCC-HPLC-DAD for the preparation of 5-HMF, neomangiferin and mangiferin from Anemarrhena asphodeloides bunge. J Pharm Biomed 44:96–100CrossRefGoogle Scholar
- 19.Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar