Molecular Biology Reports

, Volume 38, Issue 1, pp 675–681 | Cite as

Specific post-transcriptional inhibition of mRNA for ligand binding chain of IgE high affinity receptor

  • Sobia Rana
  • Ishrat Waheed
  • Muhammad Imran


IgE high affinity receptor (FcεRI) plays an important role in triggering type I allergic reactions. In this study, we have investigated the ability of four synthetic and sequence-specific RNA interfering antisense oligodeoxynucleotides (AS-ODNs) to reduce the expression of FcεRIα gene in granulocytes of allergy sufferers in vitro. Only AS1 out of four AS-ODNs specifically inhibited the FcεRIα gene expression and the dose response assay revealed that AS1 was capable of specific inhibition of target mRNA expression over a linear concentration range without affecting the expression of house keeping genes such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Together, these results indicate that sequence-specific RNA interfering ODNs can be effectively used to silence the expression of key genes like IgE high affinity receptor that are involved in chronic inflammatory diseases.


High affinity receptor of IgE (FcεRI) Alpha subunit of FcεRI (FcεRIα) Antisense oligodeoxynucleotides (AS-ODNs) 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Molecular and Cell Biology Laboratory, Department of Physiology and Cell BiologyUniversity of Health Sciences (UHS)LahorePakistan
  2. 2.Department of Pharmacology and Therapeutics, Riphah Institute of Pharmaceutical SciencesRiphah International UniversityIslamabadPakistan

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