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Molecular Biology Reports

, Volume 37, Issue 1, pp 289–295 | Cite as

Plasma homocysteine and DNA damage profiles in normal and obese subjects in the Pakistani population

  • Shazia A. Bukhari
  • Muhammad I. Rajoka
  • Saeed A. Nagra
  • Zia Ur Rehman
Article

Abstract

Dependence of plasma total homocysteine (tHcy) and DNA damage profiles on melanodialdehyde (MDA), oxidative stress, liver function tests (LFT), and lipids was studied in non-obese and obese subjects in the Pakistani population. Development of obesity is influenced by both genetic, biochemical and environmental factors. Plasma homocysteine (Hcy) and DNA damage profiles play a pivotal role in its progression. We studied 160 obesity patients and 160 lean subjects. Leukocytes were evaluated for DNA damage by comet assay and blood plasma for biochemical properties using commercial kits. Plasma Hcy level and DNA damage were strongly correlated with triglycerides (P < 0.000), LDL-cholesterol (P < 0.001), systolic blood pressure (P < 0.001), cholesterol (P < 0.004), MDA (P < 0.004) and total oxygen stress (P < 0.004) in obese individuals. Both Hyc and DNA damage were negatively associated with total anti-oxidant response and globulin. Both Hcy profile and DNA damage may have a role in the endothelium damage even in the normal range and are related to triglycerides, ALT, MDA, TOS, HDL- and LDL-cholesterol in the Pakistani population.

Keywords

DNA damage Homocysteine MDA Total oxygen stress 

Notes

Acknowledgments

These studies were supported by Higher Education Commission, Punjab Government and University of Agriculture, Faisalabad. Ethical Committee is thanked for permission to carry out this work. Lab staff and Departmental statistician are thanked for their assistance.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Shazia A. Bukhari
    • 1
  • Muhammad I. Rajoka
    • 2
  • Saeed A. Nagra
    • 3
  • Zia Ur Rehman
    • 4
  1. 1.Center for Agricultural Biochemistry and BiotechnologyUniversity of AgricultureFaisalabadPakistan
  2. 2.National Institute for Biotechnology & Genetic EngineeringFaisalabadPakistan
  3. 3.Institute of ChemistryUniversity of the PunjabLahorePakistan
  4. 4.Department of Physiology and PharmacologyUniversity of AgricultureFaisalabadPakistan

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