Molecular Biology Reports

, Volume 37, Issue 4, pp 2043–2048 | Cite as

Development of cell culture system from the giant freshwater prawn Macrobrachium rosenbergii (de Man)

  • Mukunda Goswami
  • Wazir S. Lakra
  • T. Rajaswaminathan
  • Gourav Rathore


A new cell culture system (MRH) was developed for the first time from 2 months old freshwater prawn, Macrobrachium rosenbergii. Primary cultures were developed from heart tissues by explant culture technique. Cell outgrowth was obtained from the heart explant after 14 days of explant culture. The culture medium used was Leibovitz-15 supplemented with 20% Fetal Bovine Serum along with 1% prawn hemolymph serum, 0.1% glucose, 0.5% NaCl and antibiotics (Penicillin 10,000 Units ml−1, Streptomycin 10,000 μg ml−1, Amphotericin B 500 mg ml−1) with a final osmomolality of 470–550 mmol kg−1. The pH of the growth medium found suitable for the growth of the cells was 7.20. The viability of cells was found to be 60% when revived after a month of storage in liquid nitrogen.


Macrobrachium rosenbergii Cell culture Giant freshwater prawn MRH 



The first author acknowledges the financial support from CSIR, New Delhi and the Director, National Bureau of Fish Genetic Resources, Lucknow for the research facilities.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Mukunda Goswami
    • 1
  • Wazir S. Lakra
    • 1
  • T. Rajaswaminathan
    • 1
  • Gourav Rathore
    • 1
  1. 1.National Bureau of Fish Genetic ResourcesLucknowIndia

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