Molecular Biology Reports

, Volume 37, Issue 4, pp 1717–1723 | Cite as

Pre and post cloning characterization of a β-1,4-endoglucanase from Bacillus sp.

  • Sumra Afzal
  • Mahjabeen Saleem
  • Riffat Yasmin
  • Mamoona Naz
  • Muhammad Imran


Consistent with its precloning characterization from the cellulolytic Bacillus sp., β-1,4-endoglucanase purified from the recombinant E. coli exhibited maximum activity at 60°C and pH 7.0. It was highly specific for CMC hydrolysis, with stability up to 70°C and over a pH range of 6.0–8.0. The K m and V max values for CMCase activity of the enzyme were 4.1 mg/ml and 25 μmole/ml min−1, respectively. The purified enzyme was a monomer of 65 kDa, as determined by SDS-PAGE. The presence of sucrose and IPTG in fermentation media increased the endoglucanase activity of the recombinant enzyme to 5.2-folds as compared with that of the actual one.


Bacillus sp. β-1,4-Endoglucanase Cloning 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Sumra Afzal
    • 1
  • Mahjabeen Saleem
    • 1
  • Riffat Yasmin
    • 2
  • Mamoona Naz
    • 1
  • Muhammad Imran
    • 3
  1. 1.Institute of Biochemistry and BiotechnologyUniversity of the PunjabLahorePakistan
  2. 2.Sheikh Zayed Fedral Postgraduate Medical InstituteLahorePakistan
  3. 3.Molecular and Cell Biology Laboratory, Department of Physiology and Cell BiologyUniversity of Health Sciences (UHS)LahorePakistan

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