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Molecular Biology Reports

, Volume 37, Issue 1, pp 39–45 | Cite as

Molecular cloning, sequence characterization and tissue transcription profile analyses of two novel genes: LCK and CDK2 from the Black-boned sheep (Ovis aries)

  • Hongman Yu
  • Shanna Chen
  • Dongmei Xi
  • Yiduo He
  • Qin Liu
  • Huaming Mao
  • Weidong Deng
Article

Abstract

The complete coding sequences of two sheep genes—LCK and CDK2 were amplified using the rapid amplification of cDNA ends method based on three sheep EST sequences whose translated amino acids contain the domain PTKc_Lck_BIk and S_TKc domain, respectively. The sequence analyses of these two genes revealed that the sheep LCK gene encodes a protein of 509 amino acids which has high homology with the lymphocyte-specific protein tyrosine kinase (LCK) of eight species: bovine (99%), human (96%), dog (96%), Aotus nancymaae (95%), mouse (94%), rat (91%), horse (91%) and chicken (81%). The sheep CDK2 gene encodes a protein of 298 amino acids which has high homology with the cyclin-dependent kinase 2 (CDK2) of ten species: bovine (100%), goat (100%), rat (99%), mouse (99%), Chinese hamster (99%), dog (98%), golden hamster (98%), human (98%), horse (98%) and rhesus monkey (98%). The tissue transcription profile analyses indicated that that the Black-boned sheep LCK and CDK2 genes are generally but differentially expressed in the detected tissues including in tissues including spleen, muscle, skin, kidney, lung, liver and heart. These data serve as a foundation for further insight into these two genes.

Keywords

Black-boned sheep LCK CDK2 Tissue transcription profile 

Notes

Acknowledgments

This work was supported by grants from the Natural Science Foundation Key Project of Yunnan Province (No. 20006C005Z), 863 Key Program (2008AA101001), National Nature Science Foundation of China (Project Nos. 30760177 and 30860042) and the Candidates of the Young and Middle Aged Academic and Technical Leaders of Yunnan Province (2008PY039, to Dr. W. D. Deng).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Hongman Yu
    • 1
    • 2
  • Shanna Chen
    • 1
  • Dongmei Xi
    • 3
  • Yiduo He
    • 3
  • Qin Liu
    • 3
  • Huaming Mao
    • 3
  • Weidong Deng
    • 3
  1. 1.School of Life ScienceYunnan UniversityKunmingChina
  2. 2.Faculty of Agriculture and BiotechnologyYunnan Agricultural UniversityKunmingChina
  3. 3.Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and TechnologyYunnan Agricultural UniversityKunmingChina

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