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Molecular cloning, sequence characterization and tissue transcription profile analyses of two novel genes: LCK and CDK2 from the Black-boned sheep (Ovis aries)

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Abstract

The complete coding sequences of two sheep genes—LCK and CDK2 were amplified using the rapid amplification of cDNA ends method based on three sheep EST sequences whose translated amino acids contain the domain PTKc_Lck_BIk and S_TKc domain, respectively. The sequence analyses of these two genes revealed that the sheep LCK gene encodes a protein of 509 amino acids which has high homology with the lymphocyte-specific protein tyrosine kinase (LCK) of eight species: bovine (99%), human (96%), dog (96%), Aotus nancymaae (95%), mouse (94%), rat (91%), horse (91%) and chicken (81%). The sheep CDK2 gene encodes a protein of 298 amino acids which has high homology with the cyclin-dependent kinase 2 (CDK2) of ten species: bovine (100%), goat (100%), rat (99%), mouse (99%), Chinese hamster (99%), dog (98%), golden hamster (98%), human (98%), horse (98%) and rhesus monkey (98%). The tissue transcription profile analyses indicated that that the Black-boned sheep LCK and CDK2 genes are generally but differentially expressed in the detected tissues including in tissues including spleen, muscle, skin, kidney, lung, liver and heart. These data serve as a foundation for further insight into these two genes.

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Acknowledgments

This work was supported by grants from the Natural Science Foundation Key Project of Yunnan Province (No. 20006C005Z), 863 Key Program (2008AA101001), National Nature Science Foundation of China (Project Nos. 30760177 and 30860042) and the Candidates of the Young and Middle Aged Academic and Technical Leaders of Yunnan Province (2008PY039, to Dr. W. D. Deng).

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Authors and Affiliations

  1. School of Life Science, Yunnan University, 650091, Kunming, China

    Hongman Yu & Shanna Chen

  2. Faculty of Agriculture and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China

    Hongman Yu

  3. Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, 650201, Kunming, China

    Dongmei Xi, Yiduo He, Qin Liu, Huaming Mao & Weidong Deng

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  1. Hongman Yu
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  2. Shanna Chen
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  7. Weidong Deng
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Correspondence to Weidong Deng.

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Yu, H., Chen, S., Xi, D. et al. Molecular cloning, sequence characterization and tissue transcription profile analyses of two novel genes: LCK and CDK2 from the Black-boned sheep (Ovis aries). Mol Biol Rep 37, 39–45 (2010). https://doi.org/10.1007/s11033-009-9532-4

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  • DOI: https://doi.org/10.1007/s11033-009-9532-4

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