Molecular Biology Reports

, Volume 37, Issue 3, pp 1427–1433 | Cite as

Alternative splicing in exon 9 of glucocorticoid receptor pre-mRNA is regulated by SRp40

  • Xue-Bo Yan
  • Chen-Hong Tang
  • Yan Huang
  • Hui Fang
  • Zhi-Qiang Yu
  • Li-Min Wu
  • Rong-Yu Liu


Increasing evidence indicates that alternative splicing of human glucocorticoid receptor (GR) transcripts is implicated in the development of glucocorticoid resistance but the underlying mechanism was not well known. Serine/arginine-rich (SR) proteins and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 play an important role in the spliceosome assembly. In this study, we analyzed the effects of different SR proteins and hnRNP A1 on the alternative splicing of GR pre-mRNA in HeLa and 293T cells using a minigene transfection assay. Our results revealed that only SRp40 could induce a GRα to GRβ shift of pre-mRNA splicing in exon 9 in HeLa cells and this effect induced by SRp40 was further confirmed by small interfering RNA study. However, in 293T cells, SRp40 could not induce this shift. These results indicated that SRp40 may influence the alternative splicing of GR pre-mRNA to regulate the ratio of GRα to GRβ, and this effect is cell-dependent.


Glucocorticoid receptor SR proteins Minigene Alternative splicing Exon Transfection 



We showed our great thanks to Professor Francisco E. Baralle (International Centre for Genetic Engineering and Biotechnology, Trieste, Italy) for providing hnRNPA1 and SR constructs. This work was supported by the Nature Science Foundation of China (30670936).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Xue-Bo Yan
    • 1
    • 2
  • Chen-Hong Tang
    • 1
  • Yan Huang
    • 1
  • Hui Fang
    • 2
  • Zhi-Qiang Yu
    • 2
  • Li-Min Wu
    • 2
  • Rong-Yu Liu
    • 1
  1. 1.Anhui Geriatric InstituteThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  2. 2.Department of Neurobiology and Biophysics, School of Life ScienceUniversity of Science and Technology of ChinaHefeiChina

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