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Molecular Biology Reports

, 37:661 | Cite as

Cloning and characterization of the Rubisco activase gene from Ipomoea batatas (L.) Lam

  • Ke Xu
  • Bowen He
  • Shuang Zhou
  • Yi Li
  • Yizheng Zhang
Article

Abstract

A full-length cDNA of Rubisco activase (IBrcaI) was cloned from sweet potato (Ipomoea batatas (L.) Lam) using Rapid-Amplification of cDNA Ends (RACE). IBrcaI contains a 1,347 bp open reading frame encoding a protein of 439 amino acids. The sequence alignment of multiple Rubisco activase genes from sweet potato and other plants showed high homology at two previously described ATP-binding sites. Western blot analysis indicated that there are two Rubisco activase proteins in sweet potato. Expression of IBrcaI was only detected in leaves. In the 14 h light and 10 h dark photoperiods, maximal and minimal IBrcaI mRNA expression levels were detected at 8:00 in the morning and at midnight, respectively.

Keywords

Gene cloning Gene expression Rubisco activase Ipomoea batatas (L.) Lam 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ke Xu
    • 1
  • Bowen He
    • 1
  • Shuang Zhou
    • 1
  • Yi Li
    • 1
  • Yizheng Zhang
    • 1
  1. 1.Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life ScienceSichuan UniversityChengduPeople’s Republic of China

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