Abstract
Macroautophagy is a regulated bulk degradation process of cellular components, mainly long-lived proteins or cytoplasmic organelles. Nutrient depletion is a classic inducer of macroautophagy. In this report, we have induced heat-mediated macroautophagy in several cell lines in the absence of nutrient depletion. Heat treatment increased the autophagic markers LC3-I and LC3-II at the protein levels. Interestingly, expression of a constitutively active HSF1 mutant suppressed basal LC3-II protein level and heat-induced increase of LC3-II. Our results provide evidence that heat is a potent inducer of macroautophagy in mammalian cells, and implicate the negative role of active HSF1 in this process.
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Acknowledgments
We thank Dr. Richard Voellmy for his encouragement and for critically reading this manuscript, and Dr. Bingren Hu, Department of Neurology, University of Miami School of Medicine, for providing LC3 antibody. This work was supported by funds provided by the Chinese Academy of Sciences (Guangzhou Institute of Biomedicine and Health), the Science and Technology divisions of Guangdong and Guangzhou, National Natural Science Foundation of China (30600274) and Science and Technology Projects of Guangdong Province (2007B030701001).
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Zhao, Y., Gong, S., Shunmei, E. et al. Induction of macroautophagy by heat. Mol Biol Rep 36, 2323–2327 (2009). https://doi.org/10.1007/s11033-009-9451-4
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DOI: https://doi.org/10.1007/s11033-009-9451-4