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Molecular Biology Reports

, Volume 36, Issue 7, pp 1749–1756 | Cite as

Molecular cloning and characterization of a 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase gene from Cephalotaxus harringtonia

  • Junfeng Chen
  • Ying Xiao
  • Peng Di
  • Xiaojing Yu
  • Wansheng Chen
  • Lei Zhang
Article

Abstract

The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250 amino acids with a calculated mass of 26.67 kDa and an isoelectric point of 9.35. Comparative and bioinformatics analyses revealed that ChMECPS showed extensive homology with MECPSs from other plant species. Phylogenetic analysis indicated ChMECPS was more ancient than other plant MECPSs. Southern hybridization analysis of the genomic DNA showed that Chmecps was a single copy gene. Tissue expression pattern analysis revealed that ChMECPS expressed strongly in root and leaf, weakly in stem.

Keywords

2C-methyl-d-erythritol 2, 4-Cyclodiphosphate synthase (MECPS) Cephalotaxus harringtonia Non-mevalonate pathway (MEP) Rapid amplification of cDNA ends (RACE) 

Notes

Acknowledgments

This research was financial supported by National Natural Science Foundation of China (20572130, 30600807), Shanghai Natural Science Foundation (04ZR14051), Shanghai Science and Technology Committee and Shanghai Rising-Star Program (06QB14010).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Pharmacognosy, School of PharmacySecond Military Medical UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Pharmacy, Changzheng HospitalSecond Military Medical UniversityShanghaiPeople’s Republic of China

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