Molecular Biology Reports

, 36:1695 | Cite as

Heterologous synthesis and assembly of functional LHII antenna complexes from Rhodovulum sulfidophilum in Rhodobacter sphaeroides mutant

  • Wanneng Wang
  • Zongli Hu
  • Xuqing Chen
  • Zhiping Zhao
  • Jinzhe Li
  • Guoping Chen


The light harvesting complexes, including LHII and LHI, are the important components of photosynthetic apparatus. Rhodovulum (Rdv.) sulfidophilum and Rhodobacter (R.) sphaeroides belong to two genera of photosynthetic bacteria, and they are very different in some physiological characteristics and light harvesting complexes structure. The LHII structural genes (pucBsAs) from Rdv. sulfidophilum and the LHI structural genes (pufBA) from R. sphaeroides were amplified, and cloned into an expression vector controlled by puc promoter from R. sphaeroides, which was then introduced into LHI and LHII-minus R. sphaeroides mutants; the transconjugant strains synthesized heterologous LHII and native LHI complexes, which played normal roles in R. sphaeroides. The Rdv. sulfidophilum LHII complex from pucBsAs had near-infrared absorption bands at ~801–853 nm in R. sphaeroides, and was able to transfer energy efficiently to the native LHI complex. The results show that the pucBsAs genes from Rdv. sulfidophilum could be expressed in R. sphaeroides, and the functional foreign LHII and native LHI were assembled into the membrane of R. sphaeroides.


Photosynthetic bacteria Light harvesting complexes Rhodovulum sulfidophilum Rhodobacter sphaeroides Heterologous expression 



Light-harvesting II


Light-harvesting I


Reaction center


Luria–Bertani medium


Polymerase chain reaction


Reverse transcription-PCR


Phenyl methyl sulfonyl fluoride


Ethylene diamine tetraacetic acid


Lauryl N, N-dimethylamine-N-oxide



This work was supported by the Program “863” of the Ministry of Science and Technology of the People’s Republic of China (NO:2006AA02Z138) and Natural Science Foundation of the Chongqing Science and Technology Commission (NO: CSTC,2006BA5006). We appreciate C. Neil Hunter for offering the strain DD13, and also acknowledge the intellectual support of my colleagues in our laboratory.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Wanneng Wang
    • 1
    • 2
  • Zongli Hu
    • 1
  • Xuqing Chen
    • 1
  • Zhiping Zhao
    • 1
  • Jinzhe Li
    • 1
  • Guoping Chen
    • 1
  1. 1.College of BioengineeringChongqing UniversityShapingba, ChongqingChina
  2. 2.Department of BioengineeringChongqing Institute of TechnologyYangjiaping, ChongqingChina

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