The X-STRs are important tools in forensic application, particularly in complex cases of kinship testing. In deficiency paternity testing when alleged father cannot be typed, investigation of X-STR markers yields the desired information. Blood samples were collected from unrelated individual (118 females and 94 males) and 84 trios families (father, mother and daughter). DNA extraction from whole blood was performed with Phenol chloroform method. Five X-linked STR markers DXS6800, DXS7133, DXS6797, DXS981 and GATA165B12 were selected. The amplicons were analyzed through ABI 3100 Genetic Analyzer. Pentaplex PCR system was developed for multilocus amplification at the same time. For each locus 4–9 alleles were noted. Altogether, 32 alleles were observed from five markers. Eighty-four trios families were analysed to check the mutation rate and no mutation was observed. Stutter peaks were observed maximum at locus DXS6797 (12.44%) while the minimum at locus DXS7133 (4.5%). For sensitivity study, amplification of X chromosomal short tandem repeats loci was successfully performed using 0.15 ng quantity of DNA as template. In conclusion; this pentaplex represents a convenient method to study X chromosome markers. It works with reasonable amounts of DNA and is suitable for paternity cases.
X-Chromosome Short tandem repeats (STRs) Multiplex PCR Punjabi population data Pakistan
This is a preview of subscription content, log in to check access.
Szibor R, Krawczak M, Hering S et al (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74PubMedGoogle Scholar
Moretti TR, Baumstark AL, Defenbaugh DA et al (2001) Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J Forensic Sci 46:647–660PubMedGoogle Scholar
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
Bar W, Brinkmann B, Budowle B et al (1997) DNA recommendations. Further report of the DNA commission of the ISFH regarding the use of short tandem repeat systems. International society for forensic haemogenetics. Int J Legal Med 110(4):175–176. doi:10.1007/s004140050061PubMedCrossRefGoogle Scholar
Turrina S, De Leo (2003) Population data of three X-chromosomal STRs: DXS7132, DXS7133 and GATA172D05 in North Italy. J Forensic Sci 48:1428–1429PubMedGoogle Scholar
Poetsch M, Petersmann A, Woenckhaus C et al (2005) Evaluation of allelic alterations in short tandem repeats an different kinds of solid tumors possible pitfalls in forensic casework. Forensic Sci Int 101:198–201Google Scholar
Wiegand P, Berger B, Edelmann J et al (2003) Population genetic comparisons of three X-chromosomal STRs. Int J Legal Med 117:62–65PubMedGoogle Scholar
Toni C, Presciuttini S, Spinetti I et al (2003) Population data of four X-chromosome markers in Tuscany, and their use in a deficiency paternity case. Forensic Sci Int 137:215–216PubMedGoogle Scholar
John BM (2005) Forensic DNA typing. Elsevier Academic, UKGoogle Scholar
Kishida T, Wang W, Fukuda M et al (1997) Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus (Japanese). J Leg Med 51:67–69Google Scholar
Desmarais D, Zhong Y, Chakraborty R et al (1998) Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA). J Forensic Sci 43:1046–1049PubMedGoogle Scholar