Molecular Biology Reports

, Volume 36, Issue 6, pp 1491–1496 | Cite as

Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp



By differential screening, we cloned the CagCNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181–192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed.


CNBP Embryogenesis Organogenesis Spermatogenesis Gibel carp 



Cellular nucleic acid-binding protein


Carassius auratus gibelio cellular nucleic acid-binding protein


Carassius auratus gibelio Vasa


Carassius auratus gibelio Apo-14


Carassius auratus gibelio Mdkb


Carassius auratus gibelio Fetuin-B




Open reading frame


Untranslated region


Switch mechanism at the 5′ end of RNA templates


Phosphate saline buffer


Propidium iodide


Tetramethylr-hodamine isothiocyanate


Fluorescein isothiocyanate



This work was supported by grants from the National Major Basic Research Program (2004CB117401).


  1. 1.
    Klug A, Rhodes D (1987) ‘Zinc fingers’: a novel protein motif for nucleic acid recognition. Trends Biochem Sci 12:464–469. doi: 10.1016/0968-0004(87)90231-3 CrossRefGoogle Scholar
  2. 2.
    Saotome Y, Winter CG, Hirsh D (1995) A widely expressed novel C2H2 zinc-finger protein with multiple consensus phosphorylation sites is conserved in mouse and man. Gene 152:233–238. doi: 10.1016/0378-1119(94)00717-7 PubMedCrossRefGoogle Scholar
  3. 3.
    Armas P, Cabada MO, Calcaterra NB (2001) Primary structure and developmental expression of Bufo arenarum cellular nucleic acid-binding protein: changes in subcellular localization during early embryogenesis. Dev Growth Differ 43:13–23. doi: 10.1046/j.1440-169x.2001.00551.x PubMedCrossRefGoogle Scholar
  4. 4.
    Rajavashisth TB, Taylor AK, Andalibi A, Svenson KL, Lusis AJ (1989) Identification of a zinc finger protein that binds to the sterol regulatory element. Science 245:640–643. doi: 10.1126/science.2562787 PubMedCrossRefGoogle Scholar
  5. 5.
    Warden CH, Krisans SK, Purcell-Huynh D, Leete LM, Daluiski A, Diep A et al (1994) Mouse cellular nucleic acid binding proteins: a highly conserved family identified by genetic mapping and sequencing. Genomics 24:14–19. doi: 10.1006/geno.1994.1576 PubMedCrossRefGoogle Scholar
  6. 6.
    van Heumen WR, Claxton C, Pickles JO (1997) Sequence and tissue distribution of chicken cellular nucleic acid binding protein cDNA. Comp Biochem Physiol, Part B Biochem. Mol Biol 118:659–665CrossRefGoogle Scholar
  7. 7.
    Yasuda J, Mashiyama S, Makino R, Ohyama S, Sekiya T, Hayashi K (1995) Cloning and characterization of rat cellular nucleic acid binding protein (CNBP) cDNA. DNA Res 2:45–49. doi: 10.1093/dnares/2.1.45 PubMedCrossRefGoogle Scholar
  8. 8.
    Flink IL, Blitz I, Morkin E (1998) Characterization of cellular nucleic acid binding protein from Xenopus laevis: expression in all three germ layers during early development. Dev Dyn 211:123–130. doi :10.1002/(SICI)1097-0177(199802)211:2<123::AID-AJA1>3.0.CO;2-JPubMedCrossRefGoogle Scholar
  9. 9.
    Armas P, Cachero S, Lombardo VA, Weiner A, Allende ML, Calcaterra NB (2004) Zebrafish cellular nucleic acid-binding protein: gene structure and developmental behaviour. Gene 337:151–161. doi: 10.1016/j.gene.2004.04.031 PubMedCrossRefGoogle Scholar
  10. 10.
    Liu JX, Gui JF (2005) Expression pattern and developmental behaviour of cellular nucleic acid-binding protein (CNBP) during folliculogenesis and oogenesis in fish. Gene 365:181–192. doi: 10.1016/j.gene.2005.04.035 CrossRefGoogle Scholar
  11. 11.
    Chen W, Liang YQ, Deng WJ, Shimizu K, Ashique AM, Li E, Li YP (2003) The zinc-finger protein CNBP is required for forebrain formation in the mouse. Development 130:1367–1379. doi: 10.1242/dev.00349 PubMedCrossRefGoogle Scholar
  12. 12.
    Shimizua K, Chen W, Ashiquea AM, Moroia R, Li YP (2003) Molecular cloning, developmental expression, promoter analysis and functional characterization of the mouse CNBP gene. Gene 307:51–62. doi: 10.1016/S0378-1119(03)00406-2 CrossRefGoogle Scholar
  13. 13.
    Abe Y, Chen W, Huang W, Nishino M, Li YP (2006) CNBP regulates forebrain formation at organogenesis stage in chick embryos. Dev Biol 295:116–127. doi: 10.1016/j.ydbio.2006.03.012 PubMedCrossRefGoogle Scholar
  14. 14.
    Zhou L, Wang Y, Gui JF (2000) Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio Bloch) as revealed by RAPD assays. J Mol Evol 51:498–506PubMedGoogle Scholar
  15. 15.
    Dong CH, Yang ST, Yang ZA, Zhang L, Gui JF (2004) A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish. Dev Biol 265:341–354. doi: 10.1016/j.ydbio.2003.08.028 PubMedCrossRefGoogle Scholar
  16. 16.
    Yang L, Gui JF (2004) Positive selection on multiple antique allelic lineages of transferrin in the polyploid Carassius auratus. Mol Biol Evol 21(7):1264–1277. doi: 10.1093/molbev/msh121 PubMedCrossRefGoogle Scholar
  17. 17.
    Yin J, Xia JH, Du XZ, Liu J, Zhou L, Hong YH, Gui JF (2007) Developmental expression of CagMdkb during gibel carp embryogenesis. Int J Dev Biol 51:761–769. doi: 10.1387/ijdb.072346jy PubMedCrossRefGoogle Scholar
  18. 18.
    Xu HY, Hong YH, Gui JF (2005) Isolation and characterization of a vasa gene in gibel carp (Carassius auratus gibelio). Dev Dyn 233:872–882. doi: 10.1002/dvdy.20410 PubMedCrossRefGoogle Scholar
  19. 19.
    Liu JX, Zhai YH, Geng FS, Xia JH, Gui JF (2008) Molecular characterization and expression pattern of fetuin-B in gibel carp (Carassius auratus gibelio). Biochem Genet doi: 10.1007/s10528-008-9176-4
  20. 20.
    Yang ZA, Liu NA, Lin S (2001) A zebrafish forebrain-specific zinc finger gene can induce ectopic dlx2 and dlx6 expression. Dev Biol 231:138–148. doi: 10.1006/dbio.2000.0139 PubMedCrossRefGoogle Scholar
  21. 21.
    Cecconi S, Ciccarelli C, Barberi M, Macchiareli G, Canipari R (2004) Granulosa cell-oocyte interactions. Eur J Obstet Gynecol Reprod Biol 115S:S19–S22. doi: 10.1016/j.ejogrb.2004.01.010 CrossRefGoogle Scholar

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© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental BiologyInstitute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of SciencesWuhanChina

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