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Molecular Biology Reports

, Volume 36, Issue 6, pp 1251–1256 | Cite as

Apolipoprotein E polymorphism in normal Han Chinese population: frequency and effect on lipid parameters

  • Shu Liang
  • Min Pan
  • Hai-Hua Geng
  • Hui Chen
  • Li-Qun Gu
  • Xiao-Tong Qin
  • Jin-Jun Qian
  • Jian-Hua Zhu
  • Chun-Feng Liu
Article

Abstract

Apolipoprotein E (ApoE) genotypes were studied in order to determine the prevalence and effect on lipid parameters in normal Han Chinese population. Fragments of ApoE gene forth exon containing codon 112 and 158 polymorphic locus were amplified by PCR, and then digested with Cfo I endonuclease. Genotypes and alleles frequencies of 168 healthy Han Chinese were calculated. The frequency of genotypes ε3/3, ε3/4, and ε2/3 was found to be 75.00, 10.70, and 11.90%, respectively, and 0.60, 1.20, and 0.60% for ε2/2, ε2/4, and ε4/4. The effects of ApoE genotypes and alleles on lipid parameters were analyzed. The effects of ApoE alleles on TC, LDL-C, ApoB was: along a decreasing gradient ε4 > ε3 > ε2. The effect of ε4 allele was to increase serum levels of TC, LDL-C and ApoB, and ε2 allele had an effect opposite to that of ε4 allele. Results obtained in this study indicate that ApoE polymorphism is an independent genetic factor on individual serum levels of lipids and apolipoproteins.

Keywords

Apolipoprotein E Genetic polymorphism Chinese 

Abbreviations

ApoAI

Apolipoprotein AI

ApoB

Apolipoprotein B

ApoE

Apolipoprotein E

CHD

Coronary heart disease

CM

Chylomicron

HDL-C

High density lipoprotein-cholesterol

LDL-C

Low density lipoprotein-cholesterol

Lp(a)

Lipoprotein (a)

TC

Serum levels of total cholesterol

TG

Triglyceride

VLDL

Very low density lipoprotein

References

  1. 1.
    Davignon J, Gregg RE, Sing CF (1988) Apolipoprotein E polymorphism and atherosclerosis. Arteriosclerosis 8:1–21PubMedGoogle Scholar
  2. 2.
    Eichner JE, Dunn ST, Perveen G, Thompson DM, Stewart KE, Stroehla BC (2002) Apolipoprotein E polymorphism and cardiovascular disease: a HuGE review. Am J Epidemiol 155:487–495. doi: 10.1093/aje/155.6.487 PubMedCrossRefGoogle Scholar
  3. 3.
    Mahfouz RA, Sabbagh AS, Zahed LF et al (2006) Apolipoprotein E gene polymorphism and allele frequencies in the Lebanese population. Mol Biol Rep 33:145–149. doi: 10.1007/s11033-006-6260-x PubMedCrossRefGoogle Scholar
  4. 4.
    Pallaud C, Gueguen R, Sass C et al (2001) Genetic influences on lipid metabolism trait variability within the Stanislas Cohort. J Lipid Res 42:1879–1890PubMedGoogle Scholar
  5. 5.
    Haddy N, De Bacquer D, Chemaly MM et al (2002) The importance of plasma apolipoprotein E concentration in addition to its common polymorphism on inter-individual variation in lipid levels: results from Apo Europe. Eur J Hum Genet 10:841–850. doi: 10.1038/sj.ejhg.5200864 PubMedCrossRefGoogle Scholar
  6. 6.
    Kao JT, Tsai KS, Chang CJ, Huang PC (1995) The effects of apolipoprotein E polymorphism on the distribution of lipids and lipoproteins in the Chinese population. Atherosclerosis 114:55–59. doi: 10.1016/0021-9150(94)05464-T PubMedCrossRefGoogle Scholar
  7. 7.
    Bradley WA, Gianturco SH (1986) ApoE is necessary and sufficient for the binding of large triglyceride-rich lipoproteins to the LDL receptor; apoB is unnecessary. J Lipid Res 27:40–48PubMedGoogle Scholar
  8. 8.
    Al-Khedhairy AA (2004) Apolipoprotein E polymorphism in Saudis. Mol Biol Rep 31:257–260. doi: 10.1007/s11033-005-2713-x PubMedCrossRefGoogle Scholar
  9. 9.
    Siest G, Pillot T, Regis-Bailly A et al (1995) Apolipoprotein E: an important gene and protein to follow in laboratory medicine. Clin Chem 41:1068–1086PubMedGoogle Scholar
  10. 10.
    Kitagawa K, Matsumoto M, Kuwabara K, Ohtsuki T, Hori M (2001) Delayed, but marked, expression of apolipoprotein E is involved in tissue clearance after cerebral infarction. J Cereb Blood Flow Metab 21:1199–1207. doi: 10.1097/00004647-200110000-00008 PubMedCrossRefGoogle Scholar
  11. 11.
    Wang XL, McCredie RM, Wilcken DE (1995) Polymorphisms of the apolipoprotein E gene and severity of coronary artery disease defined by angiography. Arterioscler Thromb Vasc Biol 15:1030–1034PubMedGoogle Scholar
  12. 12.
    Lai CL, Liu CK, Lin RT, Tai CT (2007) Association of apolipoprotein E polymorphism with ischemic stroke subtypes in Taiwan. Kaohsiung J Med Sci 23:491–497PubMedCrossRefGoogle Scholar
  13. 13.
    Pei WD, Zhang YH, Sun YH et al (2006) Apolipoprotein E polymorphism influences lipid phenotypes in Chinese families with familial combined hyperlipidemia. Circ J 70:1606–1610. doi: 10.1253/circj.70.1606 PubMedCrossRefGoogle Scholar
  14. 14.
    Yang SL, He BX, Liu HL et al (2004) Apolipoprotein E gene polymorphisms and risk for coronary artery disease in Chinese Xinjiang Uygur and Han population. Chin Med Sci J 19:150–154PubMedGoogle Scholar
  15. 15.
    Wenham PR, Sedky A, Spooner RJ (1991) Apolipoprotein E phenotyping: a word of caution. Ann Clin Biochem 28(Pt 6):599–605PubMedGoogle Scholar
  16. 16.
    Fernandez-Mestre MT, Yehirobi C, Montagnani S, Balbas O, Layrisse Z (2005) Genetic variability of apolipoprotein E in different populations from Venezuela. Dis Markers 21:15–19PubMedGoogle Scholar
  17. 17.
    Tang H, Yan X, Hua Y et al (2005) Distribution of apoE polymorphism in Chinese Yunnan Dehong Dai ethnic group. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 22:224–226PubMedGoogle Scholar
  18. 18.
    Sing CF, Davignon J (1985) Role of the apolipoprotein E polymorphism in determining normal plasma lipid and lipoprotein variation. Am J Hum Genet 37:268–285PubMedGoogle Scholar
  19. 19.
    Eggertsen G, Tegelman R, Ericsson S, Angelin B, Berglund L (1993) Apolipoprotein E polymorphism in a healthy Swedish population: variation of allele frequency with age and relation to serum lipid concentrations. Clin Chem 39:2125–2129PubMedGoogle Scholar
  20. 20.
    Margaglione M, Seripa D, Gravina C et al (1998) Prevalence of apolipoprotein E alleles in healthy subjects and survivors of ischemic stroke: an Italian case–control study. Stroke 29:399–403PubMedGoogle Scholar
  21. 21.
    Smit M, de Knijff P, Rosseneu M et al (1988) Apolipoprotein E polymorphism in The Netherlands and its effect on plasma lipid and apolipoprotein levels. Hum Genet 80:287–292. doi: 10.1007/BF01790099 PubMedCrossRefGoogle Scholar
  22. 22.
    Pablos-Mendez A, Mayeux R, Ngai C, Shea S, Berglund L (1997) Association of apo E polymorphism with plasma lipid levels in a multiethnic elderly population. Arterioscler Thromb Vasc Biol 17:3534–3541PubMedGoogle Scholar
  23. 23.
    Shin MH, Kim HN, Cui LH et al (2005) The effect of apolipoprotein E polymorphism on lipid levels in Korean adults. J Korean Med Sci 20:361–366PubMedCrossRefGoogle Scholar
  24. 24.
    Zaman MM, Ikemoto S, Yoshiike N, Date C, Yokoyama T, Tanaka H (1997) Association of apolipoprotein genetic polymorphisms with plasma cholesterol in a Japanese rural population. The Shibata study. Arterioscler Thromb Vasc Biol 17:3495–3504PubMedGoogle Scholar
  25. 25.
    Muros M, Rodriguez-Ferrer C (1996) Apolipoprotein E polymorphism influence on lipids, apolipoproteins and Lp(a) in a Spanish population underexpressing apo E4. Atherosclerosis 121:13–21. doi: 10.1016/0021-9150(95)06643-8 PubMedCrossRefGoogle Scholar
  26. 26.
    Tai DY, Su FH, Chang KH, Lee-Chen GJ (1999) Human apolipoprotein E: correlation of polymorphisms and serum lipid concentrations in Chinese. Zhonghua Yi Xue Za Zhi (Taipei) 62:133–139Google Scholar
  27. 27.
    Liu HC, Hong CJ, Wang SJ et al (1999) ApoE genotype in relation to AD and cholesterol: a study of 2, 326 Chinese adults. Neurology 53:962–966PubMedGoogle Scholar
  28. 28.
    Wong SY, Lau EM, Li M, Chung T, Sham A, Woo J (2005) The prevalence of Apo E4 genotype and its relationship to bone mineral density in Hong Kong Chinese. J Bone Miner Metab 23:261–265. doi: 10.1007/s00774-004-0593-0 PubMedCrossRefGoogle Scholar
  29. 29.
    Katzman R, Zhang MY, Chen PJ et al (1997) Effects of apolipoprotein E on dementia and aging in the Shanghai Survey of Dementia. Neurology 49:779–785PubMedGoogle Scholar
  30. 30.
    Tan CE, Tai ES, Tan CS et al (2003) APOE polymorphism and lipid profile in three ethnic groups in the Singapore population. Atherosclerosis 170:253–260. doi: 10.1016/S0021-9150(03)00232-6 PubMedCrossRefGoogle Scholar
  31. 31.
    Xia Y, Sass C, Shen X, Siest G, Visvikis S (2000) Associations of apolipoprotein E concentration and polymorphism with lipids and apolipoprotein levels in Chinese from Beijing and Shanghai. Clin Chem Lab Med 38:655–659. doi: 10.1515/CCLM.2000.094 PubMedCrossRefGoogle Scholar
  32. 32.
    Utermann G, Steinmetz A, Weber W (1982) Genetic control of human apolipoprotein E polymorphism: comparison of one- and two-dimensional techniques of isoprotein analysis. Hum Genet 60:344–351. doi: 10.1007/BF00569216 PubMedCrossRefGoogle Scholar
  33. 33.
    Inamdar PA, Kelkar SM, Devasagayam TP, Bapat MM (2000) Apolipoprotein E polymorphism in non-insulin-dependent diabetics of Mumbai, India and its effect on plasma lipids and lipoproteins. Diabetes Res Clin Pract 47:217–223. doi: 10.1016/S0168-8227(99)00126-6 PubMedCrossRefGoogle Scholar
  34. 34.
    Hsueh WC, Mitchell BD, Hixson JE, Rainwater DL (2000) Effects of the ApoE polymorphism on plasma lipoproteins in Mexican Americans. Ann Epidemiol 10:524–531. doi: 10.1016/S1047-2797(00)00074-0 PubMedCrossRefGoogle Scholar
  35. 35.
    Jemaa R, Elasmi M, Naouali C et al (2006) Apolipoprotein E polymorphism in the Tunisian population: frequency and effect on lipid parameters. Clin Biochem 39:816–820. doi: 10.1016/j.clinbiochem.2006.04.018 PubMedCrossRefGoogle Scholar
  36. 36.
    Peng DQ, Zhao SP, Wang JL (1999) Lipoprotein (a) and apolipoprotein E epsilon 4 as independent risk factors for ischemic stroke. J Cardiovasc Risk 6:1–6PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Shu Liang
    • 1
    • 2
  • Min Pan
    • 3
  • Hai-Hua Geng
    • 3
  • Hui Chen
    • 2
  • Li-Qun Gu
    • 2
  • Xiao-Tong Qin
    • 3
  • Jin-Jun Qian
    • 1
  • Jian-Hua Zhu
    • 3
  • Chun-Feng Liu
    • 1
  1. 1.Department of NeurologySecond Affiliated Hospital of Soochow UniversitySuzhouPeople’s Republic of China
  2. 2.Department of OphthalmologyAffiliated Hospital of Nantong UniversityNantongPeople’s Republic of China
  3. 3.Department of CardiologyAffiliated Hospital of Nantong UniversityNantongPeople’s Republic of China

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