Molecular Biology Reports

, Volume 36, Issue 4, pp 751–756 | Cite as

Full-length coding sequences, polymorphism and chromosomal localizations of the porcine EDG4 and EDG7 genes

  • T. L. Shan
  • K. Li
  • Z. L. Tang
  • S. L. Yang
  • Y. H. Ma
  • W. J. Guan
  • D. Z. Guo


EDG4 and EDG7 are identified as cellular receptors for lysophosphatidic acid (LPA), belonging to the endothelial cell differentiation gene (EDG) family of G protein-coupled receptors (GPCR) which play an important role in the function of LPA. In this study, we presented the complete coding sequences of porcine EDG4 and EDG7 genes. The nucleotide sequences and the predicted protein sequences share high sequence identity with other mammals. Spatial expression analysis by reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that porcine EDG4 and EDG7 genes are mainly expressed in brain, liver, spleen, lung, kidney, large intestine, small intestine, but absent in muscle tissues. Radiation hybrid mapping data indicated that EDG4 and EDG7 map to q2.1 of pig chromosome 2 (SSC2) and q2.6–3.2 of pig chromosome6 (SSC6), respectively. A T/C single nucleotide polymorphism (SNP) in the coding sequence of porcine EDG4 was identified. A PCR-restriction fragment length polymorphism (PCR-RFLP) method was employed to genotype this locus among Guizhou Miniature, Guangxi Miniature, Laiwu, Wuzhishan, Tongcheng, Landrace and Yorkshire pigs. The association analysis suggested that the EDG4 genotype was associated with carcass length (P < 0.05) and drip loss percentage (P < 0.05) in the experimental population consisting of Tongcheng, Landrace, Yorkshire and two crossbred porcine populations (Wang et al. Biochem Genet (1–2):51–62, 2007).


Swine Physical mapping Polymorphism Association analysis EDG4 EDG7 



The authors wish to express their thanks to Dr Martine Yerle of INRA for providing the RH panel and H. Wang for his helps. This research was supported by the State Platform of Technology Infrastructure (2005DKA21101) National High Science and Technology Foundation of China (20060110Z1039), National Natural Science Foundation of China (30500300), Key Project of National Basic Research and Developmental Plan of China (G2006CB102105), The Key Project of National Natural Science of China (30330440) and Key Project of Scientific Research Foundation of Ministry of Human Resources of China for Returned Chinese Scholars.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Veterinary Internal Medicine, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanP.R. China
  2. 2.Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingP.R. China

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