Molecular Biology Reports

, Volume 36, Issue 4, pp 697–703 | Cite as

Cloning and characterization of a human LYPD7, a new member of the Ly-6 superfamily

  • Jun Ni
  • Qingyu Lang
  • Meirong Bai
  • Chaomin Zhong
  • Xinya Chen
  • Bo Wan
  • Long Yu


Members of the Ly-6 (Lymphocyte Antigen 6) protein family share one or several repeat units of the LU domain that is defined by a distinct disulfide bonding pattern between 8 or 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain-containing gene, LYPD7 (LY6/PLAUR domain containing 7), isolated from human testis cDNA library, and mapped to 2q22.3–23.3 by searching the UCSC genomic database. The LYPD7 cDNA sequence consists of 1,600 nucleotides and contains an open reading frame of 624 bp, encoding a putative protein of 207 amino acid residues. RT-PCR analysis showed that LYPD7 was especially highly expressed in testis, lung, stomach, and prostate. Subcellular localization of LYPD7 demonstrated that the protein was localized in the cytoplasm when overexpressed in Hela cells. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD7 in HEK 293T cells was able to activate the transcriptional activities of AP1 (PMA).


LYPD7 LU domain Ly-6 superfamily AP1 (PMA) 



(Lymphocyte Antigen 6)

LU domain

Ly-6 antigen/uPA receptor-like domain


LY6/PLAUR domain containing 7


Glycophosphatidyl inositol


Phorbol myristate acetate



This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).


  1. 1.
    LeClair KP, Rabin M, Nesbitt MN, Pravtcheva D, Ruddle FH, Palfree RG, Bothwell A (1987) Murine Ly-6 multigene family is located on chromosome 15. Proc Natl Acad Sci 84(6):1638–1642PubMedCrossRefGoogle Scholar
  2. 2.
    LeClair KP, Palfree RG, Flood PM, Hammerling U, Bothwell A (1986) Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J 5:3227–3234PubMedGoogle Scholar
  3. 3.
    Bamezai A (2004) Mouse Ly-6 proteins and their extended family: markers of cell differentiation and regulators of cell signaling. Archivum Immunologiae et Therapiae Experimentalis 52:255–266PubMedGoogle Scholar
  4. 4.
    Ploug M, Kjalke M, Rønne E, Weidle U, Høyer-Hansen G, Danø K (1993) Localization of the disulfide bonds in the NH2-terminal domain of the cellular receptor for human urokinase-type plasminogen activator. A domain structure belonging to a novel superfamily of glycolipid-anchored membrane proteins. J Biol Chem 268(23):17539–17546PubMedGoogle Scholar
  5. 5.
    Ploug M, Ellis V (1994) Structure-function relationships in the receptor for urokinase-type plasminogen activator. Comparison to other members of the Ly-6 family and snake venom alpha-neurotoxins. FEBS Lett 349:163–168PubMedCrossRefGoogle Scholar
  6. 6.
    Stroncek DF, Caruccio L, Bettinotti M (2004) CD177: a member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med 2:8PubMedCrossRefGoogle Scholar
  7. 7.
    Plesner T, Behrendt N, Ploug M (1997) Structure, function and expression on blood and bone marrow cells of the urokinase-type plasminogen activator receptor, uPAR. Stem Cells 15:398–408PubMedCrossRefGoogle Scholar
  8. 8.
    Ruud H Brakenhoff, Martijn Gerretsen, Ellen MC, Knippels, Mirjam van Dijk, Huib van Essen, Danie1 Olde Weghuis, Richard J Sinke, Gordon B Snow, Guus A MS van Dongen (1995) The human E48 antigen, highly homologous with the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell–cell adhesion. J Cell Biol 129:1677–1689Google Scholar
  9. 9.
    Shan X, Bourdeau A, Rhoton A, Wells DE, Cohen EH, Landgraf BE, Palfree RG (1998) Characterization and mapping to human chromosome 8q24.3 of Ly-6-related gene 9804 encoding an apparent homologue of mouse TSA-1. J Immunol 160:197–208PubMedGoogle Scholar
  10. 10.
    Adermann K, Wattler F, Wattler S, Heine G, Meyer M, Forssmann WG, Nehls M (1999) Structural and phylogenetic characterization of human SLURP-1, the first secreted mammalian member of the Ly-6/uPAR protein superfamily. Protein Sci 8:810–819PubMedCrossRefGoogle Scholar
  11. 11.
    Tsuji H, Okamoto K, Matsuzaka Y, Iizuka H, Tamiya G, Inoko H (2003) SLURP-2, a novel member of the human Ly-6 superfamily that is up-regulated in psoriasis vulgaris. Genomics 81:26–33PubMedCrossRefGoogle Scholar
  12. 12.
    Davies A, Simmons DL, Hale G, Harrison RA, Tighe H, Lachmann PJ, Waldmann H (1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. J Exp Med 170:637–654PubMedCrossRefGoogle Scholar
  13. 13.
    Tarui T, Mazar AP, Cines DB, Takada Y (2001) Urokinase-type plasminogen activator receptor (CD87) is a ligand for integrins and mediates cell-cell interaction. J Biol Chem 276:3983–3990PubMedCrossRefGoogle Scholar
  14. 14.
    Schrijvers A erretsen M, Fritz JM, van Walsum M, Quak JJ, Snow GB, van Dongen GA (1991) Evidence for a role of the monoclonal antibody E48 defined antigen in cell–cell adhesion in squamous epithelia and head and neck squamous cell carcinoma. Exp Cell Res 196:264–269CrossRefGoogle Scholar
  15. 15.
    Miwa JM, Ibanez-Tallon I, Crabtree GW, Sanchez R, Sali A, Role LW, Heintz N (1999) Lynx1, an endogenous toxin-like modulator of nicotinic acetylcholine receptors in the mammalian CNS. Neuron 23:105–114PubMedCrossRefGoogle Scholar
  16. 16.
    Westbrook VA, Farris EM, Hao Z, Coonrod SA, Flickinger CJ, Herr JC (2003) SAMP14, a novel, acrosomal membrane-associated, glycosyl phosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm–egg interaction. J Biol Chem 278:30506–30515PubMedCrossRefGoogle Scholar
  17. 17.
    Reiter RE, Gu Z, Watabe T, Thomas G, Szigeti K, Davis E, Wahl M, Nisitani S, Yamashiro J, Le Beau MM, Loda M, Witte ON (1998) Prostate stem cell antigen: a cell surface marker overexpressed in prostate cancer. Proc Natl Acad Sci 95:1735–1740PubMedCrossRefGoogle Scholar
  18. 18.
    Dumont FJ, Altmeyer A, Staruch MJ, Dijkmans R, Palfree RG, Fischer PA (1990) Role of protein kinase C in IFN-mediated Ly-6E antigen induction. J Immunol 144:3795–3803PubMedGoogle Scholar
  19. 19.
    HogenEsch H, de Geus B, Tielen F, Rozing J (1993) Constitutive expression of Ly-6.A2 on murine keratinocytes and inducible expression on TCR γδ + dendritic epidermal T cells. J Dermatol Sci5:114–121Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Jun Ni
    • 1
  • Qingyu Lang
    • 1
  • Meirong Bai
    • 1
  • Chaomin Zhong
    • 1
  • Xinya Chen
    • 1
  • Bo Wan
    • 1
  • Long Yu
    • 1
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life ScienceFudan UniversityShanghaiP.R. China

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