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Cloning and characterization of a human LYPD7, a new member of the Ly-6 superfamily

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Abstract

Members of the Ly-6 (Lymphocyte Antigen 6) protein family share one or several repeat units of the LU domain that is defined by a distinct disulfide bonding pattern between 8 or 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain-containing gene, LYPD7 (LY6/PLAUR domain containing 7), isolated from human testis cDNA library, and mapped to 2q22.3–23.3 by searching the UCSC genomic database. The LYPD7 cDNA sequence consists of 1,600 nucleotides and contains an open reading frame of 624 bp, encoding a putative protein of 207 amino acid residues. RT-PCR analysis showed that LYPD7 was especially highly expressed in testis, lung, stomach, and prostate. Subcellular localization of LYPD7 demonstrated that the protein was localized in the cytoplasm when overexpressed in Hela cells. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD7 in HEK 293T cells was able to activate the transcriptional activities of AP1 (PMA).

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Abbreviations

Ly-6:

(Lymphocyte Antigen 6)

LU domain:

Ly-6 antigen/uPA receptor-like domain

LYPD7 :

LY6/PLAUR domain containing 7

GPI:

Glycophosphatidyl inositol

PMA:

Phorbol myristate acetate

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Acknowledgments

This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, P.R. China

    Jun Ni, Qingyu Lang, Meirong Bai, Chaomin Zhong, Xinya Chen, Bo Wan & Long Yu

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  1. Jun Ni
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  2. Qingyu Lang
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  3. Meirong Bai
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  4. Chaomin Zhong
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  5. Xinya Chen
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  6. Bo Wan
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  7. Long Yu
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Corresponding author

Correspondence to Long Yu.

Additional information

Sequence date from this article has been deposited with the GenBank Data Library under Accession No.NM177964.

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Ni, J., Lang, Q., Bai, M. et al. Cloning and characterization of a human LYPD7, a new member of the Ly-6 superfamily. Mol Biol Rep 36, 697–703 (2009). https://doi.org/10.1007/s11033-008-9231-6

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  • DOI: https://doi.org/10.1007/s11033-008-9231-6

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