Cloning and characterization of a human LYPD7, a new member of the Ly-6 superfamily
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Members of the Ly-6 (Lymphocyte Antigen 6) protein family share one or several repeat units of the LU domain that is defined by a distinct disulfide bonding pattern between 8 or 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain-containing gene, LYPD7 (LY6/PLAUR domain containing 7), isolated from human testis cDNA library, and mapped to 2q22.3–23.3 by searching the UCSC genomic database. The LYPD7 cDNA sequence consists of 1,600 nucleotides and contains an open reading frame of 624 bp, encoding a putative protein of 207 amino acid residues. RT-PCR analysis showed that LYPD7 was especially highly expressed in testis, lung, stomach, and prostate. Subcellular localization of LYPD7 demonstrated that the protein was localized in the cytoplasm when overexpressed in Hela cells. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD7 in HEK 293T cells was able to activate the transcriptional activities of AP1 (PMA).
KeywordsLYPD7 LU domain Ly-6 superfamily AP1 (PMA)
(Lymphocyte Antigen 6)
- LU domain
Ly-6 antigen/uPA receptor-like domain
LY6/PLAUR domain containing 7
Phorbol myristate acetate
This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).
- 4.Ploug M, Kjalke M, Rønne E, Weidle U, Høyer-Hansen G, Danø K (1993) Localization of the disulfide bonds in the NH2-terminal domain of the cellular receptor for human urokinase-type plasminogen activator. A domain structure belonging to a novel superfamily of glycolipid-anchored membrane proteins. J Biol Chem 268(23):17539–17546PubMedGoogle Scholar
- 8.Ruud H Brakenhoff, Martijn Gerretsen, Ellen MC, Knippels, Mirjam van Dijk, Huib van Essen, Danie1 Olde Weghuis, Richard J Sinke, Gordon B Snow, Guus A MS van Dongen (1995) The human E48 antigen, highly homologous with the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell–cell adhesion. J Cell Biol 129:1677–1689Google Scholar
- 16.Westbrook VA, Farris EM, Hao Z, Coonrod SA, Flickinger CJ, Herr JC (2003) SAMP14, a novel, acrosomal membrane-associated, glycosyl phosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm–egg interaction. J Biol Chem 278:30506–30515PubMedCrossRefGoogle Scholar
- 19.HogenEsch H, de Geus B, Tielen F, Rozing J (1993) Constitutive expression of Ly-6.A2 on murine keratinocytes and inducible expression on TCR γδ + dendritic epidermal T cells. J Dermatol Sci5:114–121Google Scholar